Methods to produce oils with modified profiles of fatty acid, carotenoids and/or terpenoids in microalgal mutants are provided. Microalgal mutants produce the oil containing fatty acids, carotenoids and/or terpenoids of a modified profile with a disruption or ablation of one or more alleles of an endogenous polynucleotide or comprising an exogeneous gene are also provided.

The present application discloses the use of diatom in rice planting and a cultivation method of rice in symbiosis with diatom and relates to the technical field of rice planting. The use of diatom in rice planting is achieved by adding diatom to an irrigated paddy field and performing co-cultivation during rice planting. Co-cultivation of diatom and rice can effectively achieve the beneficial effects of increased yield/silicon, thicker straw, and increased lodging resistance in rice, and can reduce soil degradation, reduce the risk of heavy metal pollution and increase soil fertility.

A method for preparing a solid medium platform for cultivating algae, comprising obtaining an algae culture solution (“ACS”) at approximately room temperature of a specific volumetric quantity, denoted “x”; creating raw agarose solution (“AS”) by adding low melting point agarose powder (“AP”) to water of a volume equal to “x” in a container that is different from the one used for said ACS; heating and stirring AS; cooling or waiting for the AS to cool down to approximately 15-35% above the gelling temperature of the AS; adding micronutrients supportive of algae growth after AS has cooled to approximately 15-35% above the gelling temperature of the AS to create an agarose micronutrient solution (“AMS”); after the AMS has cooled, combining the AMS with ACS and pouring the mixture into an open container and stirring; apportioning the AMS/ACS mixture into cultivating container(s); and waiting for the mixture to congeal.

The present invention provides a method for improving crop yield and/or the quality of a harvested crop comprising the step of applying a composition comprising a culture of microalgae to a plant, a plant propagation material, or a locus where said plant or plant propagation material is intended to be grown.

The invention is directed to a culture medium for Haematococcus that contains combustion gases like carbon dioxide, carbon monoxide, and oxides of nitrogen or sulfur and which fixes the carbon, nitrogen or sulfur in these combustion gases into biomass and to methods providing superior biomass yields using this culture medium to culture select species of Haematococcus such as Haematococcus sp. KAU-01.

Embodiments of the present disclosure describe bioreactor systems that integrate phototrophic organism cultivation with energy harvesting, methods of using said bioreactor systems, and the like. The bioreactor systems can comprise a bioreactor, wherein the bioreactor is configured to cultivate a phototrophic organism in a liquid growth medium, and at least one transparent photovoltaic panel positioned between the bioreactor and a light source, where the transparent photovoltaic panel transmits select wavelengths of light and absorbs select wavelengths of light.

The invention is directed to a culture medium for Haematococcus that contains combustion gases like carbon dioxide, carbon monoxide, and oxides of nitrogen or sulfur and which fixes the carbon, nitrogen or sulfur in these combustion gases into biomass and to methods providing superior biomass yields using this culture medium to culture select species of Haematococcus such as Haematococcus sp. KAU-01.

A method and modified fermentation intermediate are disclosed for the production of polyunsaturated fatty acid (PUFA). The method comprises heat sterilizing a fermentation medium comprising dextrose to produce a heat sterilized fermentation medium, wherein the heat sterilizing converts at least a portion of the dextrose to DP2+ sugars. The method comprises combining the heat sterilized fermentation medium with an enzyme capable of converting DP2+ sugars to dextrose, thereby producing a modified heat sterilized fermentation medium comprising more dextrose and less DP+2 sugars than without combining the medium with the enzyme. The modified heat sterilized fermentation intermediate may be placed in contact with a microorganism to produce PUFA, wherein the microorganism is capable of utilizing dextrose to produce PUFA.

The present disclosure provides culture or fermentation media including a biomass or derivative thereof of a hemoprotein-producing C.sub.1 metabolizing non-photosynthetic bacterium, methods of culturing cells or tissue with the culture or fermentation medium, and products produced by the culturing methods including food products, food ingredients, phytoprotective bacterial cell products, and other products of interest such as vitamins, fatty acids, amino acid, carotenoids, etc.

A method of producing a transformant, which contains using an alga belonging to the genus Nannochloropsis as a host, wherein function of the following protein (A) or (B) of the alga is suppressed, inhibited or deleted: (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 50; and (B) a DNA binding protein consisting of an amino acid sequence having 70% or more identity with the amino acid sequence of the protein (A).