Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

Provided herein are methods of producing (+)-manool comprising: contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO:2; and contacting the CPP with a sclareol synthase enzyme to form (+)-manool.

Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes or that overexpress one or more heterologous transporters, leading to increased excretions of steviol glucosides of interest.

The invention is for an increased isoprenoid production by carotenoid optimization in an expression system and the carotegenic gene for optimization may be geranylgeranyl diphosphate synthase (GGPPS), phytoene synthase (PSY1), conserved CRTI or mutated CRT1.sub.A393T, BT1 of S. cerevisae. The carotogenic gene from red yeast which includes Rhodosporidium spp. Rhodotorula spp, Sporidiobolus spp., Leucosporidium spp., Sporobolomyes spp. is selected.

The present invention relates to methods for preparing patchoulol, -santalene, and sclareol in transgenic moss cells that include heterologous nucleic acid molecules encoding a polypeptide or synthase capable of using FPP or GGPP as a substrate. Methods for producing the transgenic moss cell, as well as the transgenic moss cell itself are also disclosed.

The present invention relates to a method of producing drimenol and/or drimenol derivatives by contacting at least one polypeptide with farnesyl diphosphate. The method may be performed in vitro or in vivo. The present invention also provides amino acid sequences of polypeptides useful in the method of the invention and nucleic acid encoding the polypeptides of the invention. The method further provides host cells or organisms genetically modified to express the polypeptides of the invention and useful to produce drimenol and/or drimenol derivatives.

The present invention relates to the use of alpha-ionylideneethane as an aroma compound, and to the use of an alpha-ionylideneethane synthase in the production of one or more aroma compounds. The inventive method for preparing one or more aroma compounds comprises a) providing farnesyl diphosphate and an alpha-ionylideneethane synthase as defined herein, under conditions suitable for the alpha-ionylideneethane synthase to produce alpha-ionylideneethane, b) converting farnesyl diphosphate to alpha-ionylideneethane, in vitro or in a host cell, c) optionally, converting alpha-ionylideneethane to one or more further aroma compounds, d) isolating alpha-ionylideneethane and the optionally one or more further aroma compounds and, e) optionally, purifying alpha-ionylideneethane and the optionally one or more further aroma compounds. The invention pertains also to method for scenting a product, particularly for imparting and/or enhancing an odor or flavor, in which at least one alpha-ionylideneethane synthase is used. In addition, the invention provides an aroma compound or composition and/or fragrance composition and/or perfumed or fragranced product, comprising i) at least an alpha-ionylideneethane. Further encompassed by the invention is a perfumed or fragranced product comprising at least an alpha-ionylideneethane. The invention further relates to a method for producing alpha-ionone (4-(2,6,6-trimethylcyclohex-2-en-1-yl)but-3-en-2-one), comprising the steps in the following order: a) contacting farnesyl diphosphate with at least one alpha-ionylideneethane synthase, under conditions suitable to produce at least one alpha-ionylideneethane; b) producing the at least alpha-ionylideneethane; c) exposing the at least one alpha-ionylideneethane produced in step b) to conditions suitable for oxidative cleavage of alpha-ionylideneethane to produce alpha-ionone; and d) optionally, isolating the alpha-ionone produced in step c). The invention also relates to a host cell for producing alpha-ionone (4-(2,6,6-trimethylcyclohex-2-en-1-yl)but-3-en-2-one), wherein the host cell comprises farnesyl diphosphate and a heterologous nucleic acid encoding an alpha-ionylideneethane synthase, wherein the host cell is capable of oxidatively cleaving alpha-ionylideneethane to produce alpha-ionone. Finally, the invention relates to the use of a host cell comprising farnesyl diphosphate and a heterologous nucleic acid encoding an alpha-ionylideneethane synthase, for (i) producing alpha-ionylideneethane; (ii) producing alpha-ionone; (iii) producing vitamin A; (iv) converting alpha-ionylideneethane to alpha-ionone; (v) converting alpha-ionylideneethane to vitamin A; (vi) for heterologous reco

The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.

Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.

Provided in this disclosure are engineered sesquiterpene synthases, host cells expressing engineered sesquiterpene synthases and methods for making alpha-guaiene.