Novel Stevia rebaudiana plant cultivars, a process for preparing superior compositions comprising steviol glycosides, and the advantageous use of the compositions comprising steviol glycosides in consumables, including food and beverage products, are disclosed.

A process for recovery and purification of HMOs comprising: (a) providing an HMO-containing fermentation broth comprising biomass; (b) separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream; (c) purifying the separated HMO-containing stream; (d) concentrating the separated HMO-containing stream; and (e) drying the product of steps (a)-(d) by an indirect drying method thereby forming a purified HMO, wherein steps (c)-(d) can be performed in any order.

A process for recovery and purification of HMOs comprising: (a) providing an HMO-containing fermentation broth comprising biomass; (b) separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream; (c) purifying the separated HMO-containing stream; (d) concentrating the separated HMO-containing stream; and (e) drying the product of steps (a)-(d) by an indirect drying method thereby forming a purified HMO, wherein steps (c)-(d) can be performed in any order.

The present invention relates to a method for preparation of soybean leaves having a high content of an isoflavone derivative in a dark condition and soybean leaves having a high content of an isoflavone derivative prepared thereby. Specifically, treatment of a soybean plant 20 days to 60 days after seeding with a predetermined concentration of ethylene in a dark condition was found to accumulate higher concentrations of isoflavone derivatives in soybean leaves than treatment with ethephon in a light condition, which requires a high level of energy. Therefore, when used, the method of the present invention can economically and quickly prepare soybean leaves having a very high content of isoflavones, and the soybean leaves having a high content of isoflavone derivatives, an extract of the soybean leaves, and a fraction of the extract can be advantageously used as a food and medicine material against diseases caused by estrogen unbalance and deficient antioxidant activity.

The present disclosure provides a method for extracting and separating flavonoids from Lindera aggregata leaves. The method includes: mixing Lindera aggregata leaves with an adsorbent, conducting elution with a matrix solid-phase dispersion (MSPD) extraction method, followed by concentration to obtain a Lindera aggregata leaf extract; conducting primary separation and secondary separation on the Lindera aggregata leaf extract by high-speed counter-current liquid chromatography (HSCCC), to separate quercetin-3-O-β-D-arabinofuranoside, a mixture of quercetin-3-O-β-D-glucoside and quercetin-5-O-β-D-glucoside, quercetin-3-O-rhamnopyranoside, and kaempferol-7-O-α-L-rhamnopyranoside; where a second solvent system used in the secondary separation includes ethyl acetate, n-butanol, an addictive and water, and the addictive includes cyclodextrin. The method has a short separation period, high separation efficiency, and less impurities during purification and separation of the flavonoids from the Lindera aggregata leaves.

The present disclosure provides a method for extracting and separating flavonoids from Lindera aggregata leaves. The method includes: mixing Lindera aggregata leaves with an adsorbent, conducting elution with a matrix solid-phase dispersion (MSPD) extraction method, followed by concentration to obtain a Lindera aggregata leaf extract; conducting primary separation and secondary separation on the Lindera aggregata leaf extract by high-speed counter-current liquid chromatography (HSCCC), to separate quercetin-3-O-β-D-arabinofuranoside, a mixture of quercetin-3-O-β-D-glucoside and quercetin-5-O-β-D-glucoside, quercetin-3-O-rhamnopyranoside, and kaempferol-7-O-α-L-rhamnopyranoside; where a second solvent system used in the secondary separation includes ethyl acetate, n-butanol, an addictive and water, and the addictive includes cyclodextrin. The method has a short separation period, high separation efficiency, and less impurities during purification and separation of the flavonoids from the Lindera aggregata leaves.

The invention primarily relates to the use of phosphorus containing herbicidal active ingredients (herbicides) as desiccant for (parts of) plants of the genus Saccharum, and to corresponding methods for desiccation, i.e. for the desiccating treatment. In another aspect the present invention relates to a method for increasing the amount of saccharose (sucrose) obtainable from (aerial parts of) plants of the genus Saccharum officinarum, and to a method for producing saccharose from (aerial parts of) plants of the genus Saccharum officinarum.

The invention primarily relates to the use of phosphorus containing herbicidal active ingredients (herbicides) as desiccant for (parts of) plants of the genus Saccharum, and to corresponding methods for desiccation, i.e. for the desiccating treatment. In another aspect the present invention relates to a method for increasing the amount of saccharose (sucrose) obtainable from (aerial parts of) plants of the genus Saccharum officinarum, and to a method for producing saccharose from (aerial parts of) plants of the genus Saccharum officinarum.

The present invention relates to a novel liquid-liquid extraction process of a bio-oil obtained by an improved thermochemical process. This extraction process gives rise to two distinct phases: an organic phase, with bio-oil of energetic added value, and an aqueous phase, where the following can be obtained by chemical compounds with added value: lactic acid, formic acid, hydroxymethylfurfural, furfural, levulinic acid, monosaccharides, disaccharides and compounds with antioxidant properties, among others.

The present invention relates to a novel liquid-liquid extraction process of a bio-oil obtained by an improved thermochemical process. This extraction process gives rise to two distinct phases: an organic phase, with bio-oil of energetic added value, and an aqueous phase, where the following can be obtained by chemical compounds with added value: lactic acid, formic acid, hydroxymethylfurfural, furfural, levulinic acid, monosaccharides, disaccharides and compounds with antioxidant properties, among others.