Methods and materials for genetically engineering methylotrophic yeast are provided.

The present disclosure provides genetically engineered microorganisms for the simultaneous fermentation of pentose and hexose sugars, for example, glucose and xylose. The microorganisms can be modified to express AtSWEET polypeptides, LST1 polypeptides, mutants thereof, homologs thereof or combinations thereof. Also provided are methods of co-fermenting hexose and pentose sugars, methods of increasing the conversion of lignocellulosic biomass via microbial fermentation, and methods of generating biofuel.

The present disclosure provides genetically engineered microorganisms for the simultaneous fermentation of pentose and hexose sugars, for example, glucose and xylose. The microorganisms can be modified to express AtSWEET polypeptides, LST1 polypeptides, mutants thereof, homologs thereof or combinations thereof. Also provided are methods of co-fermenting hexose and pentose sugars, methods of increasing the conversion of lignocellulosic biomass via microbial fermentation, and methods of generating biofuel.

Genetically engineered oleaginous fungi (e.g., engineered Yarrowia lipolytica) are provided for use in itaconic acid production. In some aspects, the engineered fungi comprise a transgene for expression of a cis-aconitic acid decarboxylase (CAD) enzyme and, optionally, one or more further genetic modifications. Methods and culture systems for production of itaconic acid using such fungi are also provided.

Genetically engineered oleaginous fungi (e.g., engineered Yarrowia lipolytica) are provided for use in itaconic acid production. In some aspects, the engineered fungi comprise a transgene for expression of a cis-aconitic acid decarboxylase (CAD) enzyme and, optionally, one or more further genetic modifications. Methods and culture systems for production of itaconic acid using such fungi are also provided.

A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.

Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.

Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.

The present disclosure concerns the recombinant expression of thermostable alpha-amylases in a yeast host cell, compositions and yeast products made from the recombinant yeast host cells as well as the use of the thermostable alpha-amylase for hydrolyzing starch and ultimately making a fermentation product.

The present disclosure concerns the recombinant expression of thermostable alpha-amylases in a yeast host cell, compositions and yeast products made from the recombinant yeast host cells as well as the use of the thermostable alpha-amylase for hydrolyzing starch and ultimately making a fermentation product.