A method for organ imaging, comprising: administering to a subject a diagnostic effective amount of 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-phenoxycyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate or 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate. In one embodiment, the organ includes one or more of kidney, bladder, liver, gall bladder, spleen, intestine, heart, lungs and muscle.

A composition comprising: a polymorphic form of 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-phenoxycyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-ulfonatobutyl)-3H-indol- 1- ium-5-sulfonate or 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate and an acceptable excipient.

A method for organ imaging, comprising: administering to a subject a diagnostic effective amount of 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-phenoxycyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate or 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate. In one embodiment, the organ includes one or more of kidney, bladder, liver, gall bladder, spleen, intestine, heart, lungs and muscle.

An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, angsd usage of them.

The present disclosure relates to chemical dyes useful for staining and imaging of cells. In particular, the disclosure relates to compound of Formula I, method of preparation thereof, and it's use as a fluorescent probe for staining and/or imaging mitochondria in cells, tissues or animals, resulting in a range of applications including, but not limiting, to sensing local ordering or viscosity of mitochondria, tracking mitochondrial mobility, comparing & evaluating mitochondrial function, local ordering, microviscosity and dynamics. Said dyes have additional advantages including, but not limiting, to low toxicity, longer shelf-life, generate little or no reactive species upon long term light irradiation and do not perturb the functionality of the mitochondria in cells compared to prior art dyes.

##STR00001##

There are provided a compound of Formula (1) and a labeled biological substance having the compound.

##STR00001##

A ring Z.sup.1 and a ring Z.sup.2 represent a 6-membered ring formed of a ring-constituting atom selected from a carbon atom and a nitrogen atom.

R.sup.1 to R.sup.4, R.sup.11 to R.sup.13, L.sup.1, and L.sup.2 represents specific groups, and n, ?1, and ?2 represent specific numbers.

At least one of R.sup.11, R.sup.12, or R.sup.13 contains a carboxy group or a substituent capable of being bonded to a biological substance.

the compound represented by Formula (1) has at least one structure represented by (CH.sub.2CH.sub.2O).sub.mR.sup.21, where R.sup.21 represents a specific group, and m represents a specific number.

The compound represented by Formula (1) is a neutral compound.

A color filter substrate is disclosed, including a substrate, a black matrix disposed on the substrate and multiple color resist blocks separated by the black matrix and disposed on the substrate. Each color resist block is filled with a color resist material, the color resist material includes pigment molecules, including at least one photosensitive substituent. A liquid crystal display device having the same is also disclosed. Utilizing an UV irradiation step in the CF process, the pigment molecules are aligned directionally, which can increase the transmittance of the color filter substrate, the utilization rate of the backlight, and decrease the power consumption. Under the same display brightness, the thickness of the color filter substrate can be decreased; the pigment molecules aligned directionally can make an emitting light to be along a same direction to reduce the light interference between adjacent sub-pixels having different colors to greatly decrease the color shift.

Provided are a thermal fusion-type lithographic printing plate precursor in which the visibility is excellent, ablation during laser exposure is suppressed, and the contamination of dampening water and printing ink during on-machine development is suppressed by a lithographic printing plate precursor having a support and an image-recording layer, in which the image-recording layer is capable of forming an image by infrared laser exposure, a non-exposed portion of the image-recording layer is removable by at least one of dampening water and printing ink on a printer, and the image-recording layer contains (1) a color developing system in which a hue change generated by infrared laser exposure is a/b0.6 and (2) 70% by mass or more of a thermoplastic polymer particle with respect to a solid content of the image-recording layer and a plate-making method in which the thermal fusion-type lithographic printing plate precursor is used.

An imaging device, comprising: an infrared light absorption layer including a cyanine dye represented by Chemical Formula (A) below: wherein R.sub.1 and R.sub.2 are selected from the group consisting of: a chain alkyl group, a cyclic alkyl group, a phenyl group, and a benzyl group; wherein the chain alkyl group and the cyclic alkyl group including at least one group member selected from the group consisting of: 1) a first group having one or more hydrogen atoms in a first alkyl group substituted with at least one functional group selected from the group consisting of: a halogen atom, an alkoxy group, an alkanoloxy group, an amino group, a thiol group, and a mercapto group; 2) a second group having at least one reactive group selected from the group consisting of: a vinyl group, an acrylic group, a carbonyl group, a carboxyl group, an alkenyl group, an alkenyloxy group, an alkoxycarbonyl group, a nitrile group, a carboxyl group, a carbonyl group, a sulfonyl group, a sulfamoyl group, a carbamoyl group, a benzoyloxy group, and a cyano group, wherein the reactive group is any one of introduced at a terminal alkyl group of at least one of the chain alkyl group and the cyclic alkyl group and positioned two or more carbon atoms away from an indoline ring; 3) an unsubstituted chain alkyl group; and 4) an unsubstituted cyclic alkyl group; and wherein X.sup. represents an anion.

Provided are a protective sheet and a spectacle lens including at least one dye selected from the group consisting of a dye represented by Formula (1) and a dye represented by Formula (2), and application thereof. In Formula (1), R.sup.71 to R.sup.74 each independently represent a hydrogen atom, an alkyl group, an alkoxy group, or the like. In Formula (2), R.sup.21 represents an alkyl group, an aryl group, a heterocyclic group, or the like, R.sup.22 represents a hydrogen atom, a halogen atom, an alkyl group, or the like, and at least one of R.sup.21 or R.sup.22 is an alkyl group having 4 or more carbon atoms. n represents 0 or 1, and m represents an integer of 1 to 5.

##STR00001##