The present disclosure provides a culture device and a preparation method for a cell membrane, and relates to the field of cell culture technology. The culture device for a cell membrane includes a semiconductor refrigerator, and one or more culture vessels configured to culture a cell membrane. The semiconductor refrigerator includes a first insulating substrate, a second insulating substrate, and at least one semiconductor thermocouple disposed between the first insulating substrate and the second insulating substrate. The one or more culture vessels are disposed on a side of the first insulating substrate away from the second insulating substrate.

The present disclosure provides a culture device and a preparation method for a cell membrane, and relates to the field of cell culture technology. The culture device for a cell membrane includes a semiconductor refrigerator, and one or more culture vessels configured to culture a cell membrane. The semiconductor refrigerator includes a first insulating substrate, a second insulating substrate, and at least one semiconductor thermocouple disposed between the first insulating substrate and the second insulating substrate. The one or more culture vessels are disposed on a side of the first insulating substrate away from the second insulating substrate.

The present disclosure provides a PCR substrate, a PCR chip, a PCR system, and a liquid droplets pull-out method. The PCR substrate includes a first base; a driving structure disposed on the first base and configured to drive liquid droplets to move; where the first base includes an injection region, a stretching region and an amplification region, and the driving structure is configured to enable liquid in the injection region to form liquid droplets in the stretching region and enable the liquid droplets to move in the amplification region according to a predetermined track.

A thermal cycling method and associated device is described. The method is for carrying out a polymerase chain reaction (PCR) process to amplify deoxyribonucleic acid (DNA), and the method includes: pre-heating a series of blocks to respective temperatures that correspond to different respective heating stages in a PCR process, in which each block of the series of blocks defines a respective heat transfer surface, in which the series of blocks define a sequence of positions along a path, with each position defined by a respective heat transfer surface of a respective block; and moving a PCR reaction vessel, which contains deoxyribonucleic acid (DNA) and PCR reagents, along the path into and out of each respective position in the sequence of positions according to a schedule, in which, at each respective position the PCR reaction vessel is in thermal contact with the respective heat transfer surface to equilibrate a temperature of the PCR reaction vessel to a target temperature that corresponds to a respective heating stage in the PCR process.

A thermal cycling method and associated device is described. The method is for carrying out a polymerase chain reaction (PCR) process to amplify deoxyribonucleic acid (DNA), and the method includes: pre-heating a series of blocks to respective temperatures that correspond to different respective heating stages in a PCR process, in which each block of the series of blocks defines a respective heat transfer surface, in which the series of blocks define a sequence of positions along a path, with each position defined by a respective heat transfer surface of a respective block; and moving a PCR reaction vessel, which contains deoxyribonucleic acid (DNA) and PCR reagents, along the path into and out of each respective position in the sequence of positions according to a schedule, in which, at each respective position the PCR reaction vessel is in thermal contact with the respective heat transfer surface to equilibrate a temperature of the PCR reaction vessel to a target temperature that corresponds to a respective heating stage in the PCR process.

A culture apparatus including: a heat-insulated casing including an inner case surrounding a culture space, and an outer case, the heat-insulated casing having an opening; a box-shaped heat-insulated door to open and close the opening; and, one or more heaters provided to one or more inner surfaces of the inner case and an inner surface of an inner wall surface of the heat-insulated door, the inner wall surface facing the culture space when the door is closed; the heaters including one or more first heaters turned on when the interior of the culture space is at a first temperature to incubate a culture in the culture space and when the interior of the culture space is controlled at a second temperature to make the interior of the culture space, and second heaters to be energized when the interior of the culture space is controlled at the second temperature to sterilize the interior of the culture space.

A culture apparatus including: a heat-insulated casing including an inner case surrounding a culture space, and an outer case, the heat-insulated casing having an opening; a box-shaped heat-insulated door to open and close the opening; and, one or more heaters provided to one or more inner surfaces of the inner case and an inner surface of an inner wall surface of the heat-insulated door, the inner wall surface facing the culture space when the door is closed; the heaters including one or more first heaters turned on when the interior of the culture space is at a first temperature to incubate a culture in the culture space and when the interior of the culture space is controlled at a second temperature to make the interior of the culture space, and second heaters to be energized when the interior of the culture space is controlled at the second temperature to sterilize the interior of the culture space.

A digital PCR system includes a droplet formation assembly and a droplet ejection hole assembly, the droplet ejection hole assembly being connected below the droplet formation assembly. The droplet formation assembly includes a cover plate and at least one annular step connected to a lower surface of the cover plate, and the droplet ejection hole assembly has a plurality of droplet ejection holes. The upper surface of the droplet ejection hole assembly, the lower surface of the cover plate and the annular step jointly enclose to form a droplet formation chamber. A vaporization component is provided within the droplet ejection holes, being used for vaporizing a liquid layer of a digital PCR solution in the droplet ejection holes and quickly pushing same into droplet formation oil in the droplet formation chamber so as to form digital PCR droplets.

A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.

A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.