A cylindrical or annular polymeric bioreactor is disclosed which provides enhanced mixing and aeration of the growth medium while simultaneously offering reduced mechanical shear force.

An apparatus for containing a three-dimensional cellular material surrounded by a medium that facilitates and maintains the centering of the three-dimensional cellular material throughout an assay is provided. The apparatus includes a well having an open proximal end and a closed distal end. Further, the well defines a compartment having an interior surface and a sample nesting site for containing the three-dimensional cellular material surrounded by the medium. A central indentation is located at the closed distal end of the well, a first concentric lip is located above the central indentation in a y-direction towards the open proximal end of the well, and a second concentric lip is located above the first concentric lip in the y-direction towards the open proximal end of the well. Additionally, the first concentric lip and the second concentric lip define a groove therebetween. A method of forming a three-dimensional cellular material is also provided.

An inlay for a flask comprising a bottom portion, a side portion, an intermediate portion, and a baffle element. The bottom portion comprises a downward-facing or downward-and-outwardfacing first surface for abutting a bottom inner surface of the flask. The side portion comprises an outer surface for abutting a side inner surface of the flask, wherein the outer surface faces outwards with respect to a vertical reference line intersecting the inlay. The intermediate portion mechanically connects the bottom portion and the side portion. The baffle element extends away from the bottom portion, from the side portion, and/or from the intermediate portion for extending into an inner volume of the flask. An inner angle between the first surface and the outer surface is at least 50, wherein the inner angle refers to an angle on the side of the vertical reference line in a vertical cross section through the inlay. The inlay comprises a first flexible material adapted to allow for bending said side portion inwards. The inlay is adapted to allow for folding the inlay with respect to a horizontal crease line to insert the inlay into the flask. The inlay comprises, along a vertical plane comprising the horizontal crease line, a second flexible material to allow for said folding.

A bioreactor for cell culturing comprising a container having a base portion and a side wall defining an internal volume. The bioreactor also has a lid that closes the internal volume of the container. The base is moveable toward and away from the lid. The bioreactor also includes a baffle member mounted to the lid and positioned in the internal volume of the container such that the baffle member is arranged to mix contents of the bioreactor as the base is moved relative to the lid.

Provided herein are a culture apparatus, culture systems and an alternating ionic magnetic resonance electromagnetic chamber for culturing cells, tissues or organoid bodies or for delivering a pulsating alternating ionic magnetic resonance field to an object of interest including the above or to an animal, human or plant. The culture apparatus comprises a culture unit having growth and nutrient modules and a randomizing adapter to continually randomize the gravity vector in the growth module. The culture systems further comprise the alternating ionic magnetic resonance electromagnetic chamber.

The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.

The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.

A waste processing system includes: an input module for converting input organic waste into processed input waste; a digester module, coupled to the input module, for generating biogas and digester effluent from the processed input waste, including: a digester tank; baffles affixed within the digester tank; a digester process solution in the digester tank including, hydrolytic bacteria, acidogenic bacteria, acetogenic bacteria, methanogenic bacteria, or a combination thereof, for converting the processed input waste into the biogas and the digester effluent; and an output module, coupled to the digester module, for collecting the biogas and digester effluent after a residence period.

The present disclosure relates to methods and systems for separating particulates from a liquid medium. Certain embodiments provide a method of continuously separating particulates from a liquid medium, the method comprising exposing a liquid medium comprising particulates to electrolysis so as to cause flocculation of the particulates, and continuously separating the flocculated particulates so produced from the liquid medium, thereby continuously separating the particulates from the liquid medium.

In an embodiment of the invention, aqueous growth medium in a pond can be used to grow algae which can be pumped to a primary dewatering device where the algae can be separated from the harvested growth media based on the flow of the harvested growth media and gravity. The flow through the primary de-watering device can be optimized to maintain log phase growth in the pond, while minimizing the pumping cost and maximizing the concentration of total solids in the primary de-watered algae.