A cell culture method includes culturing a cell in a culture solution that satisfies at least one of condition (I) or condition (II) below.

Condition (I): lactic acid concentration in the culture solution being lower than 7.5 mM.

Condition (II): ammonia concentration in the culture solution being lower than 2.5 mM.

Provided is a sensor cover and a sensor apparatus including the same. The present invention is a cover (10) for a sensor for measuring properties of a solution in which gas bubbles are mixed, and has a cover main body (14) disposed to surround a sensor (12), wherein a lower transmitting portion for passing solution (14a) is disposed on at least a part of the surface of the bottom side of this cover main body (14), an upper transmitting portion for passing solution (14b) is disposed on at least a part of the upper surface of the cover main body (14), many fine holes for passing solution are formed on the lower and upper transmitting portions, and the diameter of fine holes placed on the lower transmitting portion (14a) is formed to be smaller than the diameter of the fine holes placed on the upper transmitting portion (14b).

The ammonia adsorbent contains at least one substance selected from the group consisting of L-type zeolite, ferrierite, ZSM-5 type zeolite, strongly acidic cation exchange resin and Prussian blue type complex.

This culture device comprises: a duct having an inner wall surface which faces a culture space, and a mounting surface which faces the portion to be mounted to of the inner surface, the mounting surface being mounted to the portion to be mounted to, and an air passage being formed with the inner wall surface; and a microbial light source provided within the passage. A protrusion which comes into close contact with the mounting surface, and which protrudes towards the mounting surface, is formed on the portion to be mounted to, or a protrusion which comes into close contact with the portion to be mounted to and which protrudes towards the portion to be mounted to is formed on the mounting surface.

A system and method for auto-inoculating a bioreactor in a seed train process includes an expansion chamber for expanding an initial cell stock to a viable cell density, a bioreactor for inoculation with the expanded cell stock; a fluid communication path between the expansion chamber and the bioreactor; a pump for controlling fluid flow through the fluid communication path; a Raman spectrometer for generating Raman spectral data; a multivariate model providing predictions of processing variables in the expansion chamber; and a computer system for controlling the pump to effect auto-inoculation of the bioreactor from the expansion chamber, through the fluid communication path, when the computer system determines from the Raman spectral data that one or more predefined trigger events have occurred.

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO.sub.2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

The present invention provides a method for culturing cells in a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the container is composed of a flexible material, comprising culturing cells in a culture medium in the cell culture container. Also provided is a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the wall element of the container is composed of a flexible material.

The present invention relates to bioreactors for root organ cultures that enable large parts of the inoculation or biomass transfer, cultivation and harvest of the cultures to be performed outside of an aseptic environment. The present invention further relates to methods of producing and aseptically harvesting roots, and methods of producing root cultures using the aforementioned bioreactors alone or as part of an apparatus further comprising an inoculation vessel or at least one further bioreactor.

A microperifusion system includes at least one perifusate reservoir module having N receptacles and at least one port to communicate a pressurizing gas into or out from the respective perifusate reservoir module. A corresponding perifusion chamber module having M microperifusion channels is mechanically coupleable to at least one perifusate reservoir module to thereby form, when coupled, a sealed fluid communication between a selected first number of the N receptacles and a selected second number of the M microperifusion channels. A control system module is coupleable to each perifusate reservoir and each perifusion chamber to control a flow of pressuring gas communicated into or out from the perifusate reservoir sense an effect of a microperifusion test operation occurring in each microperifusion channel.

On a device state confirmation screen (100), a first sample arrangement image (111) showing a top-view image of a sample placement section in a preprocessing device for performing preprocessing of removal of proteins or the like and a second sample arrangement image (121) showing a top-view image of a sample placement section of an auto-sampler of an LC-MS are simultaneously displayed. In the respective sample arrangement images (111, 121), a placement position of a vial is indicated by a circular region (113, 122), and the same vial number A1, A2, . . . , is assigned to a vial in which one culture medium sample is accommodated and a vial in which a preprocessed sample is accommodated. Further, circular regions 113 and 122 are displayed in different display colors depending on a state of progress of the preprocessing operation and the analysis operation. As a result, an operator can visually recognize easily and assuredly the correspondence relationship between positions of a large number of vials placed in the sample placement section and positions of a large number of vials accommodating preprocessed samples placed in the auto-sampler, which prevents erroneous selection of samples.