The disclosure generally relates to a test device that detects microorganism growth by detecting a gas metabolite (e.g., carbon dioxide) produced during the growth of bacteria or other microorganism in a tested sample. The test device can contain a culture growth media separated from a detection area by a gas-permeable membrane. The gas-permeable membrane permits carbon dioxide to permeate into the detection area. The detection area includes a solidified mixture of pH indicators and a gelling agent in the form of a semi-permeable matrix. The optical properties, including the absorbance of light at various wavelengths, of the detection solution change with alterations in carbon dioxide concentration. This test device can then be placed in an incubation and optical detection instrument to monitor changes in optical properties of the detection are induced during microorganism growth in the culture medium.

A full-function artificial organ fitting body comprises a cortex layer and an organ body tissue area. The organ body tissue area comprises a growth area, a differentiation area, a docking area, a branch arterial system, a branch nervous system and a branch venous system. The branch arterial system, the branch nervous system and the branch venous system are distributed in the differentiation area and form a main body three-dimensional skeleton structure with the outer growth area and the middle docking area.

The present disclosure features methods and compositions for increasing the amount of products of cellular metabolism, e.g., proteins, by lowering the temperature of cells expressing the product at one or more steps while culturing the cells, expressing the product, and/or recovering the product.

The present invention relates to a CO2 incubator device and a cloud system connecting the CO2 incubator device with a cloud server. The device includes a plurality of removable incubation unit trays wherein each tray acts as a separate incubator and individual bioreactor. The device includes an inlet valve and an outlet valve for each tray for inflow and outflow of CO2 gas. A microprocessor is coupled to a microcontroller for controlling temperature and CO2 level in the trays based on excess CO2 level present in the trays. A cloud server is connected to the device using a communication protocol for enabling the microprocessor to transmit incubator data to the server and to modify the incubation conditions. The data is stored in a cloud database for enabling users to remotely access the incubator data. The multiple trays are accessed independently enabling multiple users to interact with the incubator.

Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.

The invention relates to a method for extracting oxygen form a reaction chamber, as well as a use of microorganisms in such a method and a photobioreactor for carrying out the method.

According to the invention, the method comprises the steps—irradiating at least one phototropic microorganism with light under anaerobic conditions in a reaction chamber, and—in situ bonding of produced oxygen by means of the microorganism by oxidation of an oxygen-utilizing substrates.

The invention provides a process for producing a fermentable gas stream from a gas source that contains one or more constituent which may be harmful to the fermentation process. To produce the fermentable gas stream, the gas stream is passed through a specifically ordered series of removal modules. The removal modules remove and/or convert various constituents found in the gas stream which may have harmful effects on downstream removal modules and/or inhibitory effects on downstream gas fermenting microorganisms. At least a portion of the fermentable gas stream is preferably capable of being passed to a bioreactor, which contains gas fermenting microorganisms, without inhibiting the fermentation process.

Provided is a cell culture vessel which allows resolution to be high in a Z-axis direction. A cell culture vessel (1) for accommodating a cell or cell tissue therein includes: a frame part (11) which is provided so as to be located at positions that correspond to respective sides of a polyhedral shape; a window part (12) which is light-transmissive and which is provided so as to be located at positions that correspond to a respective plurality of faces out of faces of the polyhedral shape; and a shaft part (17) which extends, from any of vertices of the polyhedral shape, outward in a direction that is not parallel to any of lines normal to the faces of the polyhedral shape.

A system for digesting biodigestible feed that preferably includes the steps of comminuting the feed, introducing feed, an oxygen-containing gas, an accelerant, and bacteria into a digestion zone, the bacteria being suitable for digesting the feed under aerobic, anaerobic, and anoxic conditions. The contents of the digestion zone can be changed from aerobic operation to either anoxic or anaerobic operation, or vice versa, without changing the bacteria in the digestion zone.

Provided is a method for the selective differentiation into M1 macrophages under a pressurized environment, and more particularly, a method for the selective differentiation of undifferentiated macrophages into M1 macrophages, the method including incubating the undifferentiated macrophages in an incubator under the pressurized environment. In addition, provided is a method for producing osteoclasts, the method including: incubating undifferentiated macrophages in an incubator under a pressurized environment to differentiate into M1 macrophages; and differentiating the differentiated M1 macrophages into osteoclasts.