Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

The present invention relates to novel microalgae with high productivity of loliolide, in which the Scenedesmus sp. HS4 of the present invention can be used as a biological resource to produce loliolide due to its high biomass productivity and high loliolide content, and can be used as a pharmaceutical composition or cosmetic composition which requires loliolide derived from Scenedesmus sp. HS4.

The present disclosure relates to Rhodosorus marinus and an application thereof in preparing facial masks. Rhodosorus marinus ZS2001 has the following collection information: collection institution: China Center for Type Culture Collection (CCTCC); address of the collection institution: Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province; collection date: Oct. 30, 2023; collection number: CCTCC NO: M20232086; and collection name: Rhodosorus marinus.

The invention provides recombinant algal mutants that have a genetic modification to a gene or nucleic acid sequence encoding a WD40 repeat containing protein or domain. The genetic modification of one or more nucleic acid sequences encoding a WD40 repeat containing protein or domain results in a mutant organism with increased lipid productivity and/or higher biomass productivity (as measured by total organic carbon). The genetic modification can be a gene attenuation or functional deletion. The lipid products of these mutants can be utilized as biofuels or for other specialty chemical products. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.

An assembly, methods, and network for farming and culturing green microalgae (Haematococcus pluvialis) are disclosed, comprising: a mechanical support framework; a plurality of cultivation pouches, mechanically coupled to the mechanical support framework, having arrays of cultivation pouches where green microalgae (H. pluvialis) are cultured and harvested; a plurality of light emitting diodes (LEDs), mechanically coupled and supported by the mechanical support framework and arranged in between two of any adjacent arrays of cultivation pouches, operable to provide a predetermined light intensities to each of the cultivation pouches; and an air and gas supply and distribution unit, mechanically coupled and supported by the mechanical support framework, operable to supply predetermined amounts of air and carbon dioxide (CO.sub.2) to each of the green microalgae pouches.

Docosahexaenoic acid-containing oil containing docosahexaenoic acid in a concentration of 40 wt. % or more of the total weight of fatty acids in the oil, and having an endothermic peak temperature determined by differential scanning calorimetry (DSC) of 15 C. or lower; a biomass including the same; and a method for producing docosahexaenoic acid-containing oil including obtaining a biomass by culturing microorganisms of the genus Aurantiochytrium capable of producing this docosahexaenoic acid-containing oil, recovering the biomass after culture, and extracting the oil from the biomass after recovery.

Provided herein are triglyceride oil compositions enriched in 1,3-dioleoyl-2-palmitoylglycerol (OPO). Further provided herein are methods of producing non-naturally occurring triglyceride oil compositions enriched in OPO from non-naturally occurring microorganisms and applications thereof in a variety of end products, including, for example, polyols and nutritional supplements.

According to the present disclosure, there is provided a system for culturing animal cells using a component derived from an organism such as algae as a nutrient source, and reusing the culture waste liquid. The present disclosure provides an organism or a cultured cell which undergoes a modification, in which the modification includes imparting or enhancing L-lactate utilization ability in the organism or the cultured cell. In addition, the present disclosure provides a method for culturing at least two types of cells including a cell X and a cell Y in a circulation manner, the method including: a step (a) of providing a component excreted from the cell X to the cell Y; a step (b) of providing a component derived from the cell Y to the cell X; a step (c) of culturing the cell X and the cell Y; and a step (d) of repeating the steps (a) to (c) as necessary, in which at least one of the components excreted from the cell X is an assimilable component of the cell Y, and at least one of the components derived from the cell Y is a nutritional component of the cell X.

A method for enhancing growth of a microalgal species and its biomass production yield by co-cultivating the microalgal species with a bacterial isolate under the genus of Pseudoalteromonas in separate compartments. The microalgal species is selected from Dunaliella species. The genome sequence of the Pseudoalteromonas isolate can have a nucleotide similarity of lower than 95% to that of other Pseudoalteromonas species.

The present invention provides a method for producing recombinant growth factors using an algal expression system, which offers advantages over traditional platforms, and the algae-derived growth factors can be used to formulate cell culture media for mammalian cells without the risk of pathogen contamination or endotoxins.