Provided are a novel tricyclic dilactone compound, a strain producing the same, a method of producing the tricyclic dilactone compound, and use of the tricyclic dilactone compound. The tricyclic dilactone compound has activity of inhibiting aggregation of amyloid-beta and tau proteins, activity of inhibiting apoptosis, and anti-inflammation activity, and thus may be used to prevent, treat, or improve various neurodegenerative brain diseases including Alzheimer's disease and cognitive impairment.

The disclosure relates to bacillus having an effect of inducing corn to resist Northern leaf blight and applications of the bacillus. The bacillus having an effect of inducing corn to resist Northern corn leaf blight is Bacillus megaterium which was deposited on Apr. 16, 2019 with a deposit number of CGMCC No. 17573. The Bacillus megaterium of the disclosure can induce corn to generate resistance to Northern corn leaf blight during seed germination and corn growth to activate the immune response of corns and stimulate immune control of Northern leaf blight of corn. The method described herein is long in coming does not pollute environment and soil, is a green, simplified and new control method of Northern leaf blight of corn, and has wide application and popularization prospect.

The present invention relates to a protein variant, a microorganism with enhanced carbon monoxide (CO) availability comprising the variant, and a use thereof.

The present disclosure provides methods, compositions, and kits for in vitro cultivation of catalase-negative bacteria. The present disclosure further provides catalase-negative bacteria cultivated according to the methods described herein and bacterial stocks thereof.

The present invention provides a recombinant cell for producing para-nitro-L-phenylalanine (pN-Phe). The recombinant cell comprises heterologous genes encoding heterologous enzymes. The recombinant cell expresses the heterologous enzymes and contains a native metabolite. The native metabolite is converted to the pN-Phe in the recombinant cell. The biosynthesized pN-Phe may be incorporated into a target polypeptide in the recombinant cell without requiring exposure of the recombinant cell to exogenous pN-Phe. A cell culture comprising the recombinant cell is also provided. Further provided is a method of producing pN-Phe by a recombinant cell comprising heterologous genes encoding heterologous enzymes. The method comprises expressing a native metabolite by the recombinant cell, expressing the heterologous enzymes, and converting the native metabolite to the pN-Phe in the recombinant cell. The method may further comprise incorporating the pN-Phe into the target polypeptide in the recombinant cell.

The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial ensembles, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial compositions, and compositions comprising the same, in methods for treating or preventing gastrointestinal enteropathy or dysbiosis in canines. In particular aspects, the disclosure provides methods of treating or preventing morbidity and mortality caused by GI pathogenesis or autoimmunity.

Disclosed are a novel Lactobacillus fermentum) E4 strain isolated from Kimchi which is a traditional Korean fermented food and a mass production method for the strain. The Lactobacillus fermentum E4 strain is verified to be a safe and useful probiotic strain through acid tolerance, bile acid tolerance, gelatin liquefaction, and urease tests. In addition, high DPPH free radical scavenging (%) associated with antioxidant activity was observed, and reduction in cytokines Iinterleukin-1β (IL-1β), Iinterleukin-8 (IL-8), Iinterleukin-6 (IL-6), and Toll-like receptor (TLR4), which are associated with an inflammatory response, was observed.

A strain of Pseudomonas aeruginosa 9 # and its applications are disclosed, which relates to the technical field of microorganisms. The deposit number of the Pseudomonas aeruginosa 9 # of the disclosure is CCTCC NO: M 2021178. The strain of Pseudomonas aeruginosa 9 # can inhibit the growth of the Ustilaginodea vixens strain YY7850, which is the first instance that the Pseudomonas aeruginosa strain has been found to antagonize the pathogen of rice false smut. In view of the ability of strain 9 # to inhibit the pathogen of rice false smut, the strain has the potential to be developed into a biocontrol bacterium of rice false smut.

The present disclosure relates to Salmonella Gallinarum mutant strains and uses thereof. A vaccine composition according to an aspect has no risk of recovering pathogenicity, has no residual pathogenicity due to detoxification of an endotoxin, and does not cause lesions and bacterial re-isolation, thereby exhibiting significantly improved safety compared to the existing fowl typhoid vaccines. In addition, since the vaccine composition induces a high-level immune response even when administered to young chicks, it may be used regardless of age, and as the vaccine strain may be used as a live vaccine having an excellent protective capability by itself, the vaccine composition may be useful for preventing and alleviating fowl typhoid.

The invention discloses a recombinant Corynebacterium glutamicum for efficient synthesis of highly pure hyaluronic acid and oligosaccharides thereof, belonging to the technical field of bioengineering. The recombinant Corynebacterium glutamicum constructed in the present invention can produce hyaluronic acid with a yield up to 40g/L, and a crude product purity of 95%. Addition of exogenous hyaluronic acid hydrolase and optimization of the fermentation conditions results in hyaluronic acid oligosaccharides with specific molecular weight, and can further improve the yield of hyaluronic acid to 72 g/L. The invention lays a solid foundation for the efficient synthesis of highly pure hyaluronic acid by microorganisms, and the constructed recombinant Corynebacterium glutamicum is suitable for industrial production and application.