Various embodiments disclosed herein include systems, methods, compositions, and products by process for Rapid-breeding of plants. In certain embodiments, the systems and/or methods are at least partially automated.

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

Described is a flower bouquet conferring extended freshness to an aqueous nutrient medium for keeping cut flowers, as well as a method to confer a prolonged longevity to one or more cut flowers, comprising the step of allowing one or more cut flowers, with the cut surface of the stem thereof, to be in contact with the said aqueous nutrient medium.

Provided is a method of improving plant embryo development and/or germination. The method comprises developing plant embryos in the presence of nitric oxide (NO). The method entails the step of incubating plant embryogenic suspensor mass (ESM) in, or on, a development medium containing a nitric oxide donor for a period of time to develop mature somatic embryos. The method disclosed herein improves the yield and/or the germination frequency of plant embryos as compared to plant embryos developed by conventional methods without a nitric oxide donor in the medium.

Various embodiments disclosed herein include systems, methods, compositions, and products by process for Rapid-breeding of plants. In certain embodiments, the systems and/or methods are at least partially automated.

The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.

Disclosed is a wet granulated cell culture medium and a preparation method therefor and, more specifically, to a wet granulated cell culture medium which supports the growth of mammalian cells and/or insect cells and/or plant cells and bacteria, and a preparation method therefor.

The subject invention pertains to a method of rapidly transforming a plant with a gene of interest. The method of the current invention comprises the steps of a) preparing a bacterial culture, wherein the bacterial culture comprises a vector containing the gene of interest, b) producing a plant cell suspension culture from the plant, c) contacting the plant cell suspension culture with the bacterial culture to produce a plant cell transformed with the gene of interest from the plant cell suspension culture, and e) producing a plant from the plant cell transformed with the gene of interest. The method of the current invention can be designed for a high throughput transformation and screening of a plant with a plurality of genes of interest and screening the plants to identify and obtain plants having desirable characteristics.

This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof:

##STR00001##

wherein Ar.sup.1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar.sup.2 represents phenyl substituted with halogen; R.sup.1 and R.sup.2 each represent hydrogen, alkyl, cyano, or carboxyl; R.sup.1 and R.sup.2 may together form oxo; R.sup.3 to R.sup.10 each represent hydrogen or methyl; and R.sup.3 and R.sup.4, R.sup.5 and R.sup.6, R.sup.7 and R.sup.8, and/or R.sup.9 and R.sup.10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.

The invention relates to the use of a peptide comprising a six-member ring created by the disulfide bond between two cysteines in the increase of biomass of a photosynthetic organism, for its application in the wood industry, in obtaining energy coming from renewable sources and in agriculture.