The present disclosure generally relates to the expression of T1R1+3 taste receptors in eukaryotic cells, such as U2OS cells. The disclosure also relates to the expression of a chimeric T1R3 taste receptor subunit to enable the T1R1 taste receptor subunit to achieve a higher level of sensitivity to umami compounds. Also disclosed are methods of expressing T1R1+3 taste receptors in such cells, and methods of screening for substances that modulate T1R1+3 receptor sensing, and which, therefore, may be useful as modulators of umami taste.

An object of the present invention is to provide cells that express a membrane protein as an exogenous protein but can be stably transported without a complicated culture apparatus. Cells from Polypedilum vanderplanki expressing an exogenous membrane protein are able to stably transport the membrane protein without a complex culture apparatus.

The present disclosure provides insect cell lines for expression of viral vectors, and methods of making and using the same. Provided methods employ use of identifiers that are capable of being packaged into a viral vector to select and/or identify insect cell lines with engineered sequences associated with beneficial characteristics for viral vectors production. Exemplary viral vectors include AAV vectors.

Described herein are methods for making edible fat product using insect cells. The methods include isolating a population of insect cells, which may be precursor cells, seeding the population of insect cells onto a scaffold, and contacting the population of insect cells with a free fatty acid composition or a lipid composition. The cells seeded on the scaffold accumulate lipids, resulting in an edible fat tissue. Further disclosed are a composition of matter made from the method for making edible fat cells, a food product that includes the composition of matter, and an in vitro edible fat tissue comprising a population of insect precursor cells seeded on the scaffold.

The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically-defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.

The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.

An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5 end to a 3 end: a first promoter at least used to drive gene transcription of the first ORF; a 5 part of a gene of the first ORF; an intron; and a 3 part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

The present invention relates to the technical field of genetic transformation of insect eggs. Specifically, the present invention refers to an efficient genetic editing system to obtain recombinant or genetically modified insect eggs, by incorporating genetic material directly into oocytes of female insects, which will then generate a large number of eggs with the incorporated or recombinant genetic material.

The present disclosure provides a polypeptide fragment comprising a polypeptide of a modified hemagglutinin protein, wherein said modified hemagglutinin protein comprises a linker peptide replacing the polypeptide in the cytoplasmic domain of hemagglutinin protein, wherein the hemagglutinin protein is obtained from at least one seasonal strain of influenza virus, wherein said polypeptide of the modified hemagglutinin protein is having at least 80%, or at least 90%, or at least 95%, or at least 97% sequence identity to at least one sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, and SEQ ID NO: 30.

Provided is a Spodoptera frugiperda pupa ovary cell line with high Baculovirus production. The cell line has the name of IOZCAS-Sf-1 and the deposit number of CGMCC No. 21014. Further disclosed are a method for preparing the cell line and the use of the cell line in the large-scale growth of the Baculovirus. The cell line can be used for replicating the Baculovirus, for the large-scale production of Baculovirus insecticides and for constructing a Baculovirus expression vector system.