The present invention provides a D-glucaric acid-producing bacterium and a method for producing D-glucaric acid. The present invention is characterized in that D-glucaric acid or a salt thereof is produced from one or more saccharides selected from the group consisting of D-glucose, D-gluconic acid and D-glucuronic acid with catalytic action of a specific alcohol dehydrogenase PQQ-ADH (1) and a specific aldehyde dehydrogenase PQQ-ALDH (2), and that D-glucaric acid or a salt thereof is produced by using a microorganism having the PQQ-ADH (1) and the PQQ-ALDH (2) or a processed product thereof in the presence of the one or more saccharides. The present invention can provide a microorganism having improved productivity of D-glucaric acid to be used for production of D-glucaric acid and a method for efficiently producing D-glucaric acid.

The present invention provides a D-glucaric acid-producing bacterium and a method for producing D-glucaric acid. The present invention is characterized in that D-glucaric acid or a salt thereof is produced from one or more saccharides selected from the group consisting of D-glucose, D-gluconic acid and D-glucuronic acid with catalytic action of a specific alcohol dehydrogenase PQQ-ADH (1) and a specific aldehyde dehydrogenase PQQ-ALDH (2), and that D-glucaric acid or a salt thereof is produced by using a microorganism having the PQQ-ADH (1) and the PQQ-ALDH (2) or a processed product thereof in the presence of the one or more saccharides. The present invention can provide a microorganism having improved productivity of D-glucaric acid to be used for production of D-glucaric acid and a method for efficiently producing D-glucaric acid.

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

The present invention provides methods of method of synthesizing 2,5-furan dicarboxylic acid (FDCA) and FDCA precursor molecules. The methods involve performing a chemical dehydration reaction on a gluconic acid derivative in the presence of a dehydration catalyst. In some embodiments the gluconic acid derivative can be 2-dehydro-3-deoxy gluconic acid (DHG) or an ester thereof, 2-ketogluconic acid (2KGA) or an ester thereof, and 5-ketogluconic acid (5KGA) or an ester thereof. The 2,5-furan dicarboxylic acid precursor molecule is thereby synthesized, which can be converted into FDCA. The chemical dehydration can be performed by a variety of acid basic catalysts.

This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. This disclosure describes a cell metabolically engineered for production of a mixture of at least three different oligosaccharides. Furthermore, this disclosure provides a method for the production of a mixture of at least three different oligosaccharides by a cell as well as the purification of at least one of the oligosaccharides from the cultivation.

The present invention discloses the conversion of non-edible grade organic maize or wheat into monosaccharides by enzyme hydrolysis. The generated glucose at 14-16% is used to produce natural, organic gluconic acid by microbial fermentation of three strains Aspergillus niger NCIM 545, Penicillium notatum NCIM 745 and Penicillium chrysogenum NCIM 709. These strains are improved by unique media constituents and parameters for product yield enhancement along with the reduced time of gluconic acid production by 15-20 h. Further, gluconic acid is fortified with calcium or sodium or magnesium or ferrous to produce respective gluconate salts which were processed by a set of downstream processes including spray drying to obtain in powder form. These organic gluconates have immense applications in food, pharma, feed, and construction sectors for supplying organic source as well as minerals. This route of gluconic acid and its salts production is robust simple, cost-effective and less time taking by using eco-friendly biotechnological processes.

The present invention discloses the conversion of non-edible grade organic maize or wheat into monosaccharides by enzyme hydrolysis. The generated glucose at 14-16% is used to produce natural, organic gluconic acid by microbial fermentation of three strains Aspergillus niger NCIM 545, Penicillium notatum NCIM 745 and Penicillium chrysogenum NCIM 709. These strains are improved by unique media constituents and parameters for product yield enhancement along with the reduced time of gluconic acid production by 15-20 h. Further, gluconic acid is fortified with calcium or sodium or magnesium or ferrous to produce respective gluconate salts which were processed by a set of downstream processes including spray drying to obtain in powder form. These organic gluconates have immense applications in food, pharma, feed, and construction sectors for supplying organic source as well as minerals. This route of gluconic acid and its salts production is robust simple, cost-effective and less time taking by using eco-friendly biotechnological processes.

Production of a mixture of neutral fucosylated oligosaccharides by a cell. The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. The disclosure describes a cell metabolically engineered for production of a neutral mixture of at least four different neutral fucosylated oligosaccharides. Furthermore, the disclosure provides a method for the production of a neutral mixture of at least four different neutral fucosylated oligosaccharides by a cell as well as the purification of at least one of the neutral oligosaccharides from the cultivation.

Methods and engineered hosts are disclosed that convert a lignocellulosic xylose-containing biomass source into xylonic acid and/or xylonate, which can be further processed into other useful derivatives. In particular, an exemplary engineered Bacidiomycetes, e.g., R. toruloides, host produces/expresses one or more fungal enzymes that convert xylose into xylonic acid/xylonate. Methods of using such hosts to consume pretreated lignocellulosic biomass in combination with certain native promoters and heterologous genes are also described herein.

Methods and engineered hosts are disclosed that convert a lignocellulosic xylose-containing biomass source into xylonic acid and/or xylonate, which can be further processed into other useful derivatives. In particular, an exemplary engineered Bacidiomycetes, e.g., R. toruloides, host produces/expresses one or more fungal enzymes that convert xylose into xylonic acid/xylonate. Methods of using such hosts to consume pretreated lignocellulosic biomass in combination with certain native promoters and heterologous genes are also described herein.