The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.

A yeast extract containing 0.2% or more of -Glu-Abu based on dry weight of the yeast extract is produced by culturing a yeast, such as Saccharomyces cervisiae or Candida utilis, in a medium containing a compound selected from Abu (L-2-aminobutyric acid) and -Glu-Abu (L--glutamyl-L-2-aminobutyric acid), and preparing a yeast extract from the obtained cells.

A yeast extract containing 0.2% or more of -Glu-Abu based on dry weight of the yeast extract is produced by culturing a yeast, such as Saccharomyces cervisiae or Candida utilis, in a medium containing a compound selected from Abu (L-2-aminobutyric acid) and -Glu-Abu (L--glutamyl-L-2-aminobutyric acid), and preparing a yeast extract from the obtained cells.

Provided are a novel separated polypeptide having transaminase activity, a polynucleotide encoding the polypeptide, a microorganism including the polynucleotide, and a method of deaminating an amino compound by using the polypeptide or the microorganism.

Provided are a novel separated polypeptide having transaminase activity, a polynucleotide encoding the polypeptide, a microorganism including the polynucleotide, and a method of deaminating an amino compound by using the polypeptide or the microorganism.

Methods, systems, and devices are disclosed for in vivo production or biosynthesis of metabolites in foreign cells using the combination of (i) one or more ferredoxin dependent enzyme(s) and (ii) a ferredoxin (Fd)/ferredoxin-NADP.sup.+ reductase (FNR) system. The ferredoxin dependent enzymes and the Fd/FNR system are from the same species or from a different but matching species.

Methods, systems, and devices are disclosed for in vivo production or biosynthesis of metabolites in foreign cells using the combination of (i) one or more ferredoxin dependent enzyme(s) and (ii) a ferredoxin (Fd)/ferredoxin-NADP.sup.+ reductase (FNR) system. The ferredoxin dependent enzymes and the Fd/FNR system are from the same species or from a different but matching species.

Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.

Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.

Provided is an application of trehalase in fermentative production. The trehalase has amino acid sequences shown in SEQ ID NO.6, SEQ ID NO.7, and SEQ ID NO.8. Provided are methods for producing and applying trehalase, particularly being applied in the production and fermentation of alcohol and an amino acid.