The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on an Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

Engineered polypeptides or engineered microbial cells useful in synthesizing acyl amino acids are provided. In some embodiments, engineered polypeptides or engineered microbial cells are useful in synthesizing acyl amino acids with one or more hydroxyl and/or methyl groups at one or more positions of the fatty acid portion of the acyl amino acid (e.g., at ω-1, ω-2, and/or ω-3 positions of the fatty acid portion of the acyl amino acid). Also provided are methods of making acyl amino acids using engineered polypeptides and/or engineered microbial cells.

Engineered polypeptides or engineered microbial cells useful in synthesizing acyl amino acids are provided. In some embodiments, engineered polypeptides or engineered microbial cells are useful in synthesizing acyl amino acids with one or more hydroxyl and/or methyl groups at one or more positions of the fatty acid portion of the acyl amino acid (e.g., at ω-1, ω-2, and/or ω-3 positions of the fatty acid portion of the acyl amino acid). Also provided are methods of making acyl amino acids using engineered polypeptides and/or engineered microbial cells.

Provided are a novel separated polypeptide having transaminase activity, a polynucleotide encoding the polypeptide, a microorganism including the polynucleotide, and a method of deaminating an amino compound by using the polypeptide or the microorganism.

Provided are a novel separated polypeptide having transaminase activity, a polynucleotide encoding the polypeptide, a microorganism including the polynucleotide, and a method of deaminating an amino compound by using the polypeptide or the microorganism.

Described are methods for increasing the production of small molecules in a submerged Corynebacterium culture by supplementing Corynebacterium growth medium with the non-enzymatic fraction of spent Trichoderma fermentation broth.

Described are methods for increasing the production of small molecules in a submerged Corynebacterium culture by supplementing Corynebacterium growth medium with the non-enzymatic fraction of spent Trichoderma fermentation broth.

Process for the production of an organic acid from a lignocellulosic feedstock. The process is integrated with a pulp mill and comprises: a) pre-treating a lignocellulosic feedstock with an alkaline liquor from the pulp, thereby obtaining a pretreated cellulosic feed and a black liquor; b) subjecting the pretreated cellulosic feed from step a) to enzymatic hydrolysis, thereby obtaining a saccharide feed; c) subjecting the saccharide feed from step b) to microbial fermentation using calcium oxide from the pulp mill as a neutralising agent, thereby obtaining an organic acid calcium salt; d) treating the organic acid calcium salt with sulfuric acid, thereby obtaining the organic acid; e) optionally isolating lignin from the black liquor obtained in step a), thereby obtaining lignin and weak black liquor; and f) returning the black liquor from step a) and/or the weak black liquor from step e) to the pulp mill chemical recovery process.

Process for the production of an organic acid from a lignocellulosic feedstock. The process is integrated with a pulp mill and comprises: a) pre-treating a lignocellulosic feedstock with an alkaline liquor from the pulp, thereby obtaining a pretreated cellulosic feed and a black liquor; b) subjecting the pretreated cellulosic feed from step a) to enzymatic hydrolysis, thereby obtaining a saccharide feed; c) subjecting the saccharide feed from step b) to microbial fermentation using calcium oxide from the pulp mill as a neutralising agent, thereby obtaining an organic acid calcium salt; d) treating the organic acid calcium salt with sulfuric acid, thereby obtaining the organic acid; e) optionally isolating lignin from the black liquor obtained in step a), thereby obtaining lignin and weak black liquor; and f) returning the black liquor from step a) and/or the weak black liquor from step e) to the pulp mill chemical recovery process.

A production system for a product selected from a nitrogen-containing product and a fermented and cultured product that does not involve (or can minimize) the transport of liquid ammonia can include: an ammonia synthesis apparatus in which an ammonia-containing gas is synthesized by reaction of a source gas containing hydrogen and nitrogen in the presence of a supported metal catalyst containing as a support one or more selected from the group consisting of: i) a conductive mayenite compound; ii) a two-dimensional electride compound or a precursor thereof; and iii) a complex formed of a support base containing at least one metal oxide selected from ZrO.sub.2, TiO.sub.2, CeO.sub.2, and MgO and a metal amide represented by a formula M(NH.sub.2).sub.x (where M represents one or more selected from Li, Na, K, Be, Mg, Ca, Sr, Ba, and Eu; and x represents a valence number of M) supported by the support base.