A method for preparing troxerutin ester using whole-cell catalysis belongs to the fields of biological catalysis and pharmaceutical chemistry. The method specifically includes the following steps: evenly mixing troxerutin and a mixed organic solvent containing pyridine, then adding an acyl donor and a whole-cell catalyst, and performing a reaction under oscillation at a reaction temperature of 25 C. to 55 C.; and after the reaction is finished, separating and purifying a product by column chromatography or thin-layer chromatography, so as to obtain the troxerutin ester. The invention has the advantages of mild reaction conditions, environmental friendliness, simple process, fewer side reactions and high selectivity.

A method for preparing troxerutin ester using whole-cell catalysis belongs to the fields of biological catalysis and pharmaceutical chemistry. The method specifically includes the following steps: evenly mixing troxerutin and a mixed organic solvent containing pyridine, then adding an acyl donor and a whole-cell catalyst, and performing a reaction under oscillation at a reaction temperature of 25 C. to 55 C.; and after the reaction is finished, separating and purifying a product by column chromatography or thin-layer chromatography, so as to obtain the troxerutin ester. The invention has the advantages of mild reaction conditions, environmental friendliness, simple process, fewer side reactions and high selectivity.

A method for producing a flavonoid inclusion compound, including a cleaving step including treating a sparingly soluble flavonoid having a rhamnoside structure with an enzyme having a rhamnosidase activity in the presence of a cyclodextrin to cleave a rhamnose. According to the production method of the present invention, a flavonoid inclusion compound and a composition of flavonoid glycosides having excellent solubility in water can be efficiently produced, so that the compound and composition can be suitably utilized in the fields of medicaments, foodstuff, health foods, foods for specified health use, cosmetics, and the like.

The present invention provides a novel method of manufacturing germinated plant seeds suitable for producing a large amount of phytoalexin, a method of manufacturing raw material seeds for germination induction for use in manufacture of the above germinated plant seeds, an extract composition of the germinated plant seeds and a screening method for a plant seed candidate for use in producing a target substance. The method of manufacturing raw material seeds for germination induction comprises a pre-treatment step of maintaining plant seeds under atmosphere conditions of a carbon dioxide concentration of 400 ppm or more and/or an oxygen concentration of 19 vol % or less continuously for 5 hours or more.

The invention relates to a method for producing derivatives of O--glucosylated flavonoid, comprising at least one step of incubating a glucansucrase with a flavonoid and at least one sucrose, the flavonoid being a flavonoid which is monohydroxylated or hydroxylated in a non-vicinal manner on the B cycle. The invention also relates to novel O--glucosylated flavonoid derivatives, and to the use thereof. ##STR00001##

The invention relates to a method for producing derivatives of O--glucosylated flavonoid, comprising at least one step of incubating a glucansucrase with a flavonoid and at least one sucrose, the flavonoid being a flavonoid which is monohydroxylated or hydroxylated in a non-vicinal manner on the B cycle. The invention also relates to novel O--glucosylated flavonoid derivatives, and to the use thereof. ##STR00001##

A method for producing a flavonoid inclusion compound, including a cleaving step including treating a sparingly soluble flavonoid having a rhamnoside structure with an enzyme having a rhamnosidase activity in the presence of a cyclodextrin to cleave a rhamnose. According to the production method of the present invention, a flavonoid inclusion compound and a composition of flavonoid glycosides having excellent solubility in water can be efficiently produced, so that the compound and composition can be suitably utilized in the fields of medicaments, foodstuff, health foods, foods for specified health use, cosmetics, and the like.

The present specification relates to a novel quercetin-based compound separated from post fermented tea, an isomer thereof, a pharmaceutically acceptable salt thereof, a hydrate thereof or a solvate thereof, the compound being capable of being widely used in post fermented tea-related industries and various fields in which the compound may be used.

The present specification relates to a novel quercetin-based compound separated from post fermented tea, an isomer thereof, a pharmaceutically acceptable salt thereof, a hydrate thereof or a solvate thereof, the compound being capable of being widely used in post fermented tea-related industries and various fields in which the compound may be used.

Provided are mucolytic compounds that are more effective, and/or absorbed less rapidly from mucosal surfaces, and/or are better tolerated as compared to N-acetylcysteine (NAC) and DTT. The compounds are represented by compounds of Formula I which embrace structures (Ia)-(Ib): ##STR00001##
where the structural variables are as defined herein.