The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

The invention relates to compounds that interact with heparanase, uses in heparanase screening, uses in in vitro and in vivo imaging (e g , positron emission tomography (PET) and magnetic resonance imaging (MRI)), methods of synthesis, methods of modulating heparanase activity, and methods of treating disease and disorders associated with heparanase. The compounds of the invention are also useful in treating one or more diseases or disorders associated with the function of heparanase.

The invention relates to compounds that interact with heparanase, uses in heparanase screening, uses in in vitro and in vivo imaging (e g , positron emission tomography (PET) and magnetic resonance imaging (MRI)), methods of synthesis, methods of modulating heparanase activity, and methods of treating disease and disorders associated with heparanase. The compounds of the invention are also useful in treating one or more diseases or disorders associated with the function of heparanase.

A fluorescent probe for sialidase activity detection, is a compound represented by the following general formula or a salt thereof.

##STR00001##

R.sub.1, if present, represents the same or different monovalent substituent present on a benzene ring. R.sub.2 and R.sub.3 each independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom. R.sub.4 and R.sub.5 each independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom. R.sub.6 represents a hydrogen atom, an alkyl group having 1 to 5 carbon atoms, or a fluorinated alkyl group having 1 to 5 carbon atoms. R.sub.6′ represents a hydrogen atom or an alkyl group having 1 to 6 carbon atoms, and R.sub.6′ may form, together with R.sub.3 or R.sub.5 a five to seven-membered heterocyclyl or heteroaryl containing a nitrogen atom to which R.sub.6′ is bonded.

A fluorescent probe for sialidase activity detection, is a compound represented by the following general formula or a salt thereof.

##STR00001##

R.sub.1, if present, represents the same or different monovalent substituent present on a benzene ring. R.sub.2 and R.sub.3 each independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom. R.sub.4 and R.sub.5 each independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom. R.sub.6 represents a hydrogen atom, an alkyl group having 1 to 5 carbon atoms, or a fluorinated alkyl group having 1 to 5 carbon atoms. R.sub.6′ represents a hydrogen atom or an alkyl group having 1 to 6 carbon atoms, and R.sub.6′ may form, together with R.sub.3 or R.sub.5 a five to seven-membered heterocyclyl or heteroaryl containing a nitrogen atom to which R.sub.6′ is bonded.

This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.

This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.

A method of determining activity of a target enzyme of a grain material is described. The method comprises providing a fluid extract sample of the grain material using a preselected extraction procedure, providing a dyed and/or chromogenic substrate for the target enzyme, subjecting the fluid extract sample to the substrate for a preselected incubating time, and determining the target enzyme activity of the grain material. The extraction time is relatively short while still obtaining high accuracy.

A method of determining activity of a target enzyme of a grain material is described. The method comprises providing a fluid extract sample of the grain material using a preselected extraction procedure, providing a dyed and/or chromogenic substrate for the target enzyme, subjecting the fluid extract sample to the substrate for a preselected incubating time, and determining the target enzyme activity of the grain material. The extraction time is relatively short while still obtaining high accuracy.