It is an object of the present invention to provide a fluorescence imaging probe capable of selectively visualizing target cells such as cells expressing β-galactosidase (lacZ expressing cells) at a single-cell level in a red fluorescence region, and of performing co-staining together with GFP. An intracellularly-retainable red fluorescent probe comprising a compound represented by the following formula (I) or a salt thereof: ##STR00001## wherein: A represents a monovalent group cleaved by an enzyme; R.sup.1 represents a hydrogen atom, or one to four of the same or different substituents bonded to a benzene ring; R.sup.3, R.sup.4, R.sup.5, and R.sup.6 each independently represent —CFR.sup.10R.sup.11, —CF.sub.2R.sup.12, a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom, wherein at least one of R.sup.3, R.sup.4, R.sup.5, and R.sup.6 is —CFR.sup.10R.sup.11 or —CF.sub.2R.sup.12; R.sup.2 and R.sup.7 each independently represent a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom; R.sup.8 and R.sup.9 each independently represent a hydrogen atom or an alkyl group; R.sup.10, R.sup.11, and R.sup.12 each independently represent a hydrogen atom, an alkyl group, or an alkenyl group; X represents Si(R.sup.a) (R.sup.b), wherein R.sup.a and R.sup.b each independently represent a hydrogen atom or an alkyl group; and Y is —C(═O)— or —R.sup.cC(═O)—, wherein R.sup.c is an alkylene group having 1-3 carbon atoms.

It is an object of the present invention to provide a fluorescence imaging probe capable of selectively visualizing target cells such as cells expressing β-galactosidase (lacZ expressing cells) at a single-cell level in a red fluorescence region, and of performing co-staining together with GFP. An intracellularly-retainable red fluorescent probe comprising a compound represented by the following formula (I) or a salt thereof: ##STR00001## wherein: A represents a monovalent group cleaved by an enzyme; R.sup.1 represents a hydrogen atom, or one to four of the same or different substituents bonded to a benzene ring; R.sup.3, R.sup.4, R.sup.5, and R.sup.6 each independently represent —CFR.sup.10R.sup.11, —CF.sub.2R.sup.12, a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom, wherein at least one of R.sup.3, R.sup.4, R.sup.5, and R.sup.6 is —CFR.sup.10R.sup.11 or —CF.sub.2R.sup.12; R.sup.2 and R.sup.7 each independently represent a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom; R.sup.8 and R.sup.9 each independently represent a hydrogen atom or an alkyl group; R.sup.10, R.sup.11, and R.sup.12 each independently represent a hydrogen atom, an alkyl group, or an alkenyl group; X represents Si(R.sup.a) (R.sup.b), wherein R.sup.a and R.sup.b each independently represent a hydrogen atom or an alkyl group; and Y is —C(═O)— or —R.sup.cC(═O)—, wherein R.sup.c is an alkylene group having 1-3 carbon atoms.

The purpose of the present invention is to provide a detection means whereby Staphylococcus aureus can be identified at a high accuracy. An aspect of the present invention relates to a medium for detecting S. aureus which comprises one or more kinds of nutrient components, a color developing agent capable of developing a color in the presence of α-glucosidase, a color developing agent capable of developing a color in the presence of phosphatase and 0.5 mg/cm.sup.3 or more of sodium colistin methanesulfonate. Another aspect of the present invention relates to a sheet for detecting S. aureus, said sheet comprising the aforesaid medium, and a method for detecting S. aureus with the use of the medium and sheet as described above.

The purpose of the present invention is to provide a detection means whereby Staphylococcus aureus can be identified at a high accuracy. An aspect of the present invention relates to a medium for detecting S. aureus which comprises one or more kinds of nutrient components, a color developing agent capable of developing a color in the presence of α-glucosidase, a color developing agent capable of developing a color in the presence of phosphatase and 0.5 mg/cm.sup.3 or more of sodium colistin methanesulfonate. Another aspect of the present invention relates to a sheet for detecting S. aureus, said sheet comprising the aforesaid medium, and a method for detecting S. aureus with the use of the medium and sheet as described above.

This invention comprises compositions and methods to detect and treat gastrointestinal diseases.

This invention comprises compositions and methods to detect and treat gastrointestinal diseases.

This invention describes a de novo in vitro device to measure STAP. Measurement of stool alkaline phosphatase (STAP) will be pivotal in determining the physiological as well as pharmacological effects of intestinal alkaline phosphatase (IAP), the major component of STAP. The device is described for measuring phosphatase concentration in stool. The device (chromogenic STAP Test) allows persistent contact of a stool sample for a specific period of time (e.g., 30 min) with a piece of chromatography paper (strip) impregnated with a STAP substrate (p-nitrophenyl phosphate, p-NPP), and then the developed color (yellow) is compared with standards thus providing the STAP concentration. For a permanent record, the developed color along with standards is photographed.

This invention describes a de novo in vitro device to measure STAP. Measurement of stool alkaline phosphatase (STAP) will be pivotal in determining the physiological as well as pharmacological effects of intestinal alkaline phosphatase (IAP), the major component of STAP. The device is described for measuring phosphatase concentration in stool. The device (chromogenic STAP Test) allows persistent contact of a stool sample for a specific period of time (e.g., 30 min) with a piece of chromatography paper (strip) impregnated with a STAP substrate (p-nitrophenyl phosphate, p-NPP), and then the developed color (yellow) is compared with standards thus providing the STAP concentration. For a permanent record, the developed color along with standards is photographed.

This invention is directed to compositions and methods to detect and treat gastrointestinal diseases.

Provided by the present disclosure are fluorescent proteins that can detect kinase activity. The fluorescent proteins of the present disclosure have us in, for example, detecting kinase activity in any eukaryotic cell.