The present invention relates to DNA-barcoded recombinant nucleosomes and polynucleosomes that have been engineered for use as spike-in controls for chromatin accessibility assays, chromatin mapping assays, e.g., using tethered enzymes, as well as other chromatin assays. The invention further relates to methods of using the engineered DNA-barcoded recombinant nucleosomes in chromatin accessibility assays, chromatin mapping assays, as well as other chromatin assays.

The present invention relates to DNA-barcoded recombinant nucleosomes and polynucleosomes that have been engineered for use as spike-in controls for chromatin accessibility assays, chromatin mapping assays, e.g., using tethered enzymes, as well as other chromatin assays. The invention further relates to methods of using the engineered DNA-barcoded recombinant nucleosomes in chromatin accessibility assays, chromatin mapping assays, as well as other chromatin assays.

A polypeptide probe, including: from two to four G4-binding domains, and one or more linkers disposed between every two G4-binding domains. Each G4-binding domain includes a specific motif including a sequence of amino acids

TABLE-US-00001 (SEQ ID NO: 1) PGHLKGREIGMWY.

Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. 1). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.

Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. 1). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.

The present document describes nucleic acid structures comprising a plurality of annealed motifs that are made from complementary oligonucleotides having domains with sequences complementary to other nucleotides of the motif. The annealed motifs may be anchored to surfaces, and functional elements may be attached to the annealed motifs. The nucleic acid structures may used to make sensors therefrom. The present document also describes methods to generate said nucleic acid structures.

The present document describes nucleic acid structures comprising a plurality of annealed motifs that are made from complementary oligonucleotides having domains with sequences complementary to other nucleotides of the motif. The annealed motifs may be anchored to surfaces, and functional elements may be attached to the annealed motifs. The nucleic acid structures may used to make sensors therefrom. The present document also describes methods to generate said nucleic acid structures.

The present invention provides RNA aptamer probes for detection of target genetic material and methods for using the probes. In some embodiments, the invention provides devices for the detection of the target genetic material using the probes of the preset invention. In some embodiments, the invention provides methods for designing RNA aptamer probes for detection of target genetic material. In some embodiments, the target genetic material is genetic material from a pathogen. In some embodiments the pathogen is influenza virus. In some embodiments, the devices of the present invention may be used outside of laboratory setting and do not require any specialized skills. In some embodiments, the devices of the present invention are used in conjunction with a mobile phone camera.

The present invention provides RNA aptamer probes for detection of target genetic material and methods for using the probes. In some embodiments, the invention provides devices for the detection of the target genetic material using the probes of the preset invention. In some embodiments, the invention provides methods for designing RNA aptamer probes for detection of target genetic material. In some embodiments, the target genetic material is genetic material from a pathogen. In some embodiments the pathogen is influenza virus. In some embodiments, the devices of the present invention may be used outside of laboratory setting and do not require any specialized skills. In some embodiments, the devices of the present invention are used in conjunction with a mobile phone camera.

Provided are methods and systems for detecting formation of nucleotide-specific ternary complexes comprising a DNA polymerase, a nucleic acid, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide without requiring chemical incorporation of the nucleotide into the primer. These results can even be achieved in procedures employing unlabeled, native nucleotides.