Methods for multiplexed target detection are disclosed. Signal amplification in multiplexed solid-phase phase target detection methods are disclosed.

Methods for multiplexed target detection are disclosed. Signal amplification in multiplexed solid-phase phase target detection methods are disclosed.

A biosensor comprising an electrode and inverted molecular pendulums (iMPs) is described. Each IMP includes a linker bound to the electrode, and an analyte receptor and a redox reporter both bound to the linker. The redox reporter is reactive at positive potential when the linker presents a net negative charge and reactive at negative potential when the linker presents a net positive charge. Upon application of an electric field, the biosensor is characterized by an iMPs unbound state, where no analyte is bound to the receptor, at which the iMPs are displaced towards the electrode and electron transfer from the iMPs towards the electrode occurs at an unbound electron transfer rate, and an iMPs bound state, where the analyte is bound to the receptor, at which the iMPs are displaced towards the electrode and electron transfer from the iMPs towards the electrode occurs at a bound electron transfer rate.

A biosensor comprising an electrode and inverted molecular pendulums (iMPs) is described. Each IMP includes a linker bound to the electrode, and an analyte receptor and a redox reporter both bound to the linker. The redox reporter is reactive at positive potential when the linker presents a net negative charge and reactive at negative potential when the linker presents a net positive charge. Upon application of an electric field, the biosensor is characterized by an iMPs unbound state, where no analyte is bound to the receptor, at which the iMPs are displaced towards the electrode and electron transfer from the iMPs towards the electrode occurs at an unbound electron transfer rate, and an iMPs bound state, where the analyte is bound to the receptor, at which the iMPs are displaced towards the electrode and electron transfer from the iMPs towards the electrode occurs at a bound electron transfer rate.

Described herein is a method for detecting an oligonucleotide in a sample, and in particular, to a method for detecting sense and antisense strands of an oligonucleotide duplex in a sample.

Described herein is a method for detecting an oligonucleotide in a sample, and in particular, to a method for detecting sense and antisense strands of an oligonucleotide duplex in a sample.

Provided herein are compositions and methods for electronically sequencing polynucleotides using partially double-stranded polymer bridges. The bridges may span the space between first and second electrodes. A plurality of nucleotides may be coupled to corresponding labels. A polymerase may add nucleotides to a first polynucleotide using at least a sequence of a second polynucleotide. The labels corresponding to those nucleotides respectively may hybridize to a portion of the bridge that is not double-stranded. Detection circuitry may detect a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least changes in an electrical signal through the bridge, the changes being responsive to the respective hybridizations between the non-double stranded portion of the bridge and the labels corresponding to those nucleotides.

Provided herein are compositions and methods for electronically sequencing polynucleotides using partially double-stranded polymer bridges. The bridges may span the space between first and second electrodes. A plurality of nucleotides may be coupled to corresponding labels. A polymerase may add nucleotides to a first polynucleotide using at least a sequence of a second polynucleotide. The labels corresponding to those nucleotides respectively may hybridize to a portion of the bridge that is not double-stranded. Detection circuitry may detect a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least changes in an electrical signal through the bridge, the changes being responsive to the respective hybridizations between the non-double stranded portion of the bridge and the labels corresponding to those nucleotides.

The invention relates to a mediator probe comprising a probe region and a mediator region. Furthermore, the invention relates to a system comprising a mediator probe and a detection molecule, use of that system and a method for detection of at least one target molecule.

The invention relates to a mediator probe comprising a probe region and a mediator region. Furthermore, the invention relates to a system comprising a mediator probe and a detection molecule, use of that system and a method for detection of at least one target molecule.