The present disclosure discloses a method for targeted regulation of soil microbes to synchronize heavy metal/metalloid transformation and greenhouse gas emission reduction and use. The present disclosure discovers that by compounding methionine with an organic acid salt, the obtained formulation can significantly increase the ability of the soil microbes to promote arsenic methylation in a targeted mode, and meanwhile, and the methane production is effectively reduced in the presence of the methionine. In the present disclosure, the methionine/organic acid salt formulation is further loaded into a modified biochar material of a porous structure, so as to achieve the effect of slow release. The method of the present disclosure may effectively reduce absorption of inorganic arsenic by rice, achieve a significant reduction in the proportion of inorganic arsenic in the total arsenic in the rice and inhibit methane emission of the soils and reduce methane emission at the same time.

The present disclosure discloses a method for targeted regulation of soil microbes to synchronize heavy metal/metalloid transformation and greenhouse gas emission reduction and use. The present disclosure discovers that by compounding methionine with an organic acid salt, the obtained formulation can significantly increase the ability of the soil microbes to promote arsenic methylation in a targeted mode, and meanwhile, and the methane production is effectively reduced in the presence of the methionine. In the present disclosure, the methionine/organic acid salt formulation is further loaded into a modified biochar material of a porous structure, so as to achieve the effect of slow release. The method of the present disclosure may effectively reduce absorption of inorganic arsenic by rice, achieve a significant reduction in the proportion of inorganic arsenic in the total arsenic in the rice and inhibit methane emission of the soils and reduce methane emission at the same time.

The present invention relates to a genetic tool comprising at least two different nucleic acids allowing the transformation, by homologous recombination, of a bacterium of the genus Clostridium, typically of a solventogenic bacterium.

Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins. The glycosylated proteins may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. The glycosylated proteins may be synthesized in cell-free glycoprotein synthesis (CFGpS) systems using prokaryote cell lysates that are enriched in components for glycoprotein synthesis such as oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs) including OSTs and LLOs associated with synthesis of bacterial O antigens.

Provided are a whole bacteria capsule, a preparation method therefor and a use thereof. The preparation method comprises the steps of: (1) screening fecal bacteria donors using high-throughput sequencing; (2) collecting feces of the fecal bacteria donors screened in step (1), and preparing a fecal bacterial liquid; and (3) mixing the fecal bacterial liquid prepared in step (2) with a freeze-drying protective agent, cooling, freezing and vacuum-drying the mixture, and loading the freeze-dried feces bacteria powder obtained into a capsule shell to obtain the whole bacteria capsule. By screening the fecal bacteria donors using high-throughput sequencing, fecal bacteria donors can be quickly screened for different fecal bacteria receptors, and the whole bacteria capsule prepared can accurately match with the fecal bacteria receptors.

Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise CO.sub.2 and H.sub.2 to at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries.

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

The present invention relates to a microorganism genetically modified for improved production of alanine, wherein the microorganism expresses a heterologous alaD gene coding an alanine dehydrogenase and has reduced Lrp transcription factor activity and/or expression. The present invention also relates to a method for the production of alanine using said microorganism.