The present invention relates to a method for preparing 3-hydroxypropionic acid (3-HP) and/or a method for improving the productivity of 3-HP, the methods comprising the steps of: performing high-concentration cell culturing of a 3-HP-producing strain; and (2) isolating high-concentration-cultured cells to inoculate a medium for 3-HP production with same, thereby producing 3-HP, and thus the present invention can improve the productivity and yield of 3-HP.

Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in a gene regulating nitrogen fixation or assimilation, wherein the modification in the gene regulating nitrogen fixation or assimilation results in one or more of: constitutive expression of a nifA gene in nitrogen limiting and non-nitrogen limiting conditions, activity of nifA in non-nitrogen limiting conditions, decreased uridylyl-transferase activity of GlnD, decreased adenylyl-removing activity of GlnE, and increased ammonium excretion.

Disclosed are an enhanced efficient nitrogen-fixing composite microbial system added with non-nitrogen-fixing bacteria and application thereof, belonging to the technical field of agricultural microorganisms. The present disclosure provides enhanced efficient nitrogen-fixing bacteria, including at least one selected from a group of Klebsiella MNAZ1050, Citrobacter MNAZ1397 and Pseudomonas MNAZ228; also, the disclosed enhanced efficient nitrogen-fixing composite microbial system includes nitrogen-fixing bacteria and non-nitrogen-fixing bacteria, where the nitrogen-fixing bacteria includes at least one of the above three nitrogen-fixing bacteria, and the non-nitrogen-fixing bacteria includes at least one of Acinetobacter ACZLY512 and Kluyvera AZ981.

The result of orally administering saliva derived from a Crohn’s disease patient or an ulcerative colitis patient to germ-free mice has revealed that Th1 cells markedly increased in the colons. Further, from the bacterial microbiota in the intestines of the mice in which such an increase in Th1 cells were observed, bacteria have been successfully isolated which caused strong Th1 cell induction in the colon upon intestinal colonization.

The present disclosure provides agronomically stable liquid agricultural compositions, methods of formulation thereof, and methods of application thereof. The agricultural compositions comprise nitrogen-fixing microorganisms and one or more of a buffering agent, a microbial stabilizer, and a physical stabilizer. The disclosed liquid agricultural compositions have a longer shelf life and greater ease of application than other existing dry and liquid formulations. The disclosed liquid agricultural compositions are stable for a period of thirty days or longer with low toxin accumulation and high microbial stability. The compositions are suitable for use on agricultural plant tissues or the environs thereof for providing a source of fixed atmospheric nitrogen to the agricultural plant. The compositions are used to increase crop yield and decrease yield variance.

Provided is a two-step production method for 3-HP, comprising: a first step of culturing cells at a high concentration; and a second step of producing 3-HP using the high concentration-cultured cells as a catalyst, in which during the two-step culture, the energy and/or coenzyme balance are adjusted to produce 3-HP and/or improve the productivity of 3-HP. The productivity and yield of 3-HP can be improved.

The present disclosure provides the integration of exogenous polymers to microbes to confer increased stability and viability for an extended shelf life of the desired microbes (e.g., bacteria), as compared to those microbes lacking the exogenous polymers. The microbes include transgenic microbes, non-transgenic microbes, and non-intergeneric remodeled microbes. The utilization of the taught microbial products will enable a significant expansion of the typical shelf life of microbial compositions. The microbes comprising exogenous polymers described herein are able to be combined with other agriculturally beneficial compositions. Furthermore, the disclosure provides for the addition of exogenous microbial biofilms to the aforementioned compositions.

The present disclosure is drawn to methods of utilizing nucleic acid barcodes and corresponding amplifying sites in cells in which the barcodes naturally occur. These barcodes and amplifying sites are reconfigured into a single nucleic acid cassette that provides for ease of use in tagging particular species, strains, or variants of cells, each with a different barcode. These barcodes can be used to track the colonization capabilities of the barcoded cells. The present disclosure further provides for assays that utilize natural barcodes to measure relative microbial colonization ability of a plant root system.

The present disclosure provides the integration of exogenous microbial biofilms to confer increased stability and viability for an extended shelf life of desired microbes (e.g., bacteria), as compared to those microbes in the absence of the exogenous microbial biofilms. The microbes include transgenic microbes, non-transgenic microbes, and non-intergeneric remodeled microbes. The utilization of the taught microbial products will enable a significant expansion of the typical shelf life of microbial compositions. The microbes comprising exogenous biofilms taught herein are able to be combined with other agriculturally beneficial compositions.

The present invention relates to a recombinant microorganism which is capable of simultaneously fermenting at least two sugars in a lignocellulosic saccharified liquid, and also capable of generating diol.