What is disclosed relates to the detection and identification of bacteria of the genera Salmonella and Shigella. It relates more precisely to the methods of microbiology and the culture media used for the detection, identification, isolation and/or analytical investigation of these bacteria. Relating to a method for enrichment and selective culture of bacteria of the genera Salmonella and/or Shigella contained in a biological sample. In the method, some or all of the sample is seeded in/on a culture medium including a nutrient component that favors the development and growth of the bacteria, and includes L-ornithine as a selective agent. It also covers a culture medium suitable for carrying out this method.

Provided are delivery immunostimulatory bacteria that have enhanced colonization of tumors, the tumor microenvironment and/or tumor-resident immune cells, and enhanced anti-tumor activity. The immunostimulatory bacteria are modified by deletion of genes encoding the flagella or modification of the genes so that functional flagella are not produced, and/or are modified by deletion of pagP or modification of pagP to produce inactive PagP product. As a result, the immunostimulatory bacteria are flagellin.sup. and/or pagP.sup.. The immunostimulatory bacteria optionally have additional genomic modifications so that the bacteria are adenosine or purine auxotrophs. The bacteria optionally are one or more of asd.sup., purI.sup. and msbB.sup.. The immunostimulatory bacteria, such as Salmonella species, are modified to encode immunostimulatory proteins that confer anti-tumor activity in the tumor microenvironment, and/or are modified so that the bacteria preferentially infect immune cells in the tumor microenvironment or tumor-resident immune cells and/or induce less cell death in immune cells than in other cells. Also provided are methods of inhibiting the growth or reducing the volume of a solid tumor by administering the immunostimulatory bacteria.

The disclosure discloses a method for inducing Salmonella enteritidis into VBNC state by sodium hypochlorite. The disclosure relates to the field of biotechnology, including preparing culture medium, which is BHI culture medium, and the culture medium is cooled to room temperature, and sterilized by ultraviolet for 15 min. The strain to be tested is incubated in BHI at 37 C. for 24 h in a sterile environment, and the bacterial cells are collected by centrifugation, washed with 0.85% (w/v) sterile physiological saline for 3 times, and then resuspended in 0.85% (w/v) sterile physiological saline to obtain 10.sup.9 CFU/mL bacterial suspension. The bacteria cells are treated with sodium hypochlorite stress and the VBNC cells are counted.

Provided is application of genetically engineered bacteria VNP20009-M in preparation of drugs for preventing and treating lung cancer.

Provided is application of genetically engineered bacteria VNP20009-M in preparation of drugs for preventing and treating malignant sarcoma.

The present disclosure relates to a PCR detection kit for rapidly identifying Salmonella of specific serotypes. The kit includes primers for detecting tcpS gene, the primers for detecting the tcpS gene including a forward primer having a nucleotide sequence as set forth in SEQ ID NO. 1 and a reverse primer having a nucleotide sequence as set forth in SEQ ID NO. 2. The kit can identify Salmonella of enteritidis, pullorum/gallinarum, and dublin serotypes rapidly and in a high throughput, which can be used as an auxiliary method for the conventional serotyping of Salmonella.

Described is a method for the generation of a live vaccine containing stable bacteria carrying at least three attenuating mutations and a vaccine containing bacteria obtained by said method.

The invention relates to a method for constructing an obligate anaerobic Salmonella strain, the obligate anaerobic Salmonella strain constructed by using said method, and the use thereof in tumor therapy.

The present invention relates to a tumor-targeting Salmonella gallinarum strain and the use thereof. The tumor-targeting Salmonella gallinarum strain has excellent tumor proliferation inhibitory activity and enables tumor-specific targeting, and thus can be utilized for treatment and imaging of tumors without any side effects.

Provided herein is a bacterial delivery platform that harnesses mechanisms unique to Salmonella to intracellularly deliver protein-based drugs.