The present invention relates to a strip for an improved lateral flow assay of a biological sample on a single plane and a lateral flow chromatography assay using a test device containing the same. The strip of the present invention consists of a single-pad, which can improve lateral flow assay by providing an easy and simple procedure and clear visual reading. The strip of the present invention is consisted of sample application (sample) zone and reactant-resultant zone where the reaction mixture is deposited (reactant) are all on a same plane. In addition, the present invention provides a chromatographic method wherein hemoglobin is separated from analyte by a differential chromatography on the solid phase. Any interference of detection of the result by hemoglobin is removed by the present invention. The present invention provides advantages including an easy and simple procedure with a quick and clear response.

A recombinant yeast cell capable of consuming glucose at an increased rate, and a method of efficiently producing glycolysis-derived products using the recombinant yeast cell.

The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

The present invention relates to Saccharomyces sp. capable of producing lactic acid with a decreased activity of pyruvate decarboxylase (PDC) and increased activities of aldehyde dehydrogenase (ALD) and acetyl-CoA synthetase (ACS), and a method of producing lactic acid from the culture medium obtained by culturing the microorganism.

A yeast cell capable of producing lactate, a method of preparing the yeast cell, and a method of producing lactate by using the yeast cell, wherein theyeast cell has a reduced activity of rim15 protein, igo2 protein, or a combination thereof, and an increased activity of an enzyme that catalyzes conversion from pyruvate to lactate, compared to a parent cell.

The present disclosure provides a magnetic nano-drug with double targeting vascular endothelial growth factor (VEGF)-vascular endothelial growth factor receptor (VEGFR), and a preparation method and application thereof, and belongs to the technical field of biomedicine. The magnetic nano-drug of the present disclosure includes aminated ZnFe.sub.2O.sub.4 hollow porous magnetic nano-particles, lactate dehydrogenase-silk fibroin (LDH-SF), ethylene dichloride (EDC), and n-hydroxy succinimide (NHS). A mass ratio of the aminated ZnFe.sub.2O.sub.4 hollow porous magnetic nano-particles to the EDC to the NHS to the LDH-SF is (5-10):(1-5):(1-6):(1-8).

A genetic engineered bacteria without or comprising a plurality of important metabolic enzyme related genes is provided. When the by-product or waste of fruit and vegetable is used as the culture medium, a large quantity of succinic acid or lactic acid can be produced via fermentation. A method of producing succinic acid and lactic acid using the genetic engineered bacteria is also provided.

The present disclosure provides for production of lactic acid from organic waste or biogas or methane using recombinant methanotrophic bacteria. In one embodiment, the recombinant methanotrophic bacterium includes a heterologous nucleic acid encoding for lactate dehydrogenase (Idh) enzyme. In a further embodiment, the capacity of the recombinant methanotrophic bacterium for producing lactic acid over basal level is increased by overexpression or/and down-regulation or deletion of specified genes. In another embodiment, a process for producing lactic acid from organic waste using the recombinant methanotrophic bacterium is disclosed. The present disclosure provides a cradle to cradle environment-friendly and commercially viable solution for managing organic waste.

The present invention relates to a composition for inhibiting growth of cancer stem cells, which includes an EXT1, LDHB, CD109, EFEMP2, RASIP1 or SERPINE1 gene expression inhibitor as an active ingredient, and a method of treating cancer using the same. The composition has targeted therapeutic activities against cancer stem cells important for resistance, metastasis and recurrence of breast cancer, and thus can be useful in fundamentally treating, preventing or alleviating cancer such as breast cancer by directly inhibiting expression of EXT1, LDHB, CD109, EFEMP2, RASIP1 or SERPINE1 which are very important for growth of the cancer stem cells.

The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB.