The present disclosure relates to an acetohydroxy acid synthase variant in which the feedback inhibition to L-valine is released, a polynucleotide encoding the acetohydroxy acid synthase variant, an expression vector including the polynucleotide, a microorganism producing L-valine including the acetohydroxy acid synthase variant, and a method for producing L-valine using the microorganism.

The present invention relates to a recombinant yeast having a reduced pyruvate decarboxylase activity, in the genome of which has been inserted: one or more nucleic acids encoding an acetolactate synthase or ALS, one or more nucleic acids en coding an acetolactate decarboxylase or ALD, andone or more copies of a nucleic acids encoding a NADH oxidase or NOXE.

Non-naturally occurring Zymomonas strains useful for the production of 2,3-butanediol are provided.

The present invention relates to an ALS inhibitor herbicide tolerant polyploid plants, such as B. napus or B. juncea plants, progeny and parts thereof comprising mutations in all acetolactase genes.

A system and method for controlling metabolic enzymes or pathways in cells to produce a chemical above the levels of a wild-type strain is disclosed. The system utilizes cells, including yeasts, bacteria, and molds, having at least two genes capable of being controlled bi-directionally with light, where one gene is turned from off to on when exposed to light and another gene is turned from on to off when exposed to light, the two genes reversing when the light is turned off. Cells may utilize any number of sequences that benefit chemical production, including sequences that: encode for constitutive transcription of light-activated transcription factor fusions; encode for a metabolic enzyme; encode for a repressor; induce expression of metabolic enzymes; and an endogenous or exogenous activator expressed by a constitutive promoter, inducible promoter, or gene circuit. These systems may be coupled to biosensors or protein cascade systems, enabling the monitoring or automation of the fermentation process to optimize production of a desired product. These systems may also allow for optimization and periodic operation of a bioreactor using light pulses.

The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.

A method for producing L-isoleucine at a higher yield by fermentation includes the step of using a genetic engineering method to obtain a genetically engineered strain. The genetically engineered strain has a threonine deaminase gene substantially releasing the inhibition of L-isoleucine and/or an acetylated hydroxy acid synthetase III gene substantially releasing the inhibition of L-isoleucine; and performing fermentation culture on the genetically engineered strain, adding diketobutyric acid or a raw material capable of being converted into diketobutyric acid in a culture process, and separating L-isoleucine from a culture after the end of culturing. Further provided is a genetically engineered strain for realizing high yield of L-isoleucine.

The present disclosure relates to an acetohydroxy acid synthase variant in which the feedback inhibition to L-valine is released, a polynucleotide encoding the acetohydroxy acid synthase variant, an expression vector including the polynucleotide, a microorganism producing L-valine including the acetohydroxy acid synthase variant, and a method for producing L-valine using the microorganism.

An acetic acid metabolizing ability of a recombinant yeast strain having xylose-metabolizing ability is to be improved. In such a recombinant yeast strain having xylose-metabolizing ability, the acetaldehyde dehydrogenase gene has been introduced and a gene encoding NADH dehydrogenase involved in reoxidation of cytoplasmic NADH on the mitochondrial outer membrane has been suppressed.

The present disclosure provides a method for treating rice. The method comprises the steps of: providing a domestic rice crop plant and at least one AHAS-inhibiting herbicide selected from the group comprising a sulfonylurea herbicide, a sulfonyl carboxamide herbicide, an imidazolinone herbicide, a triazolopyrimidine herbicide, a pyrimidinyloxybenzoate herbicide, and a sulfonylaminocarbonyltriazolinone herbicide; applying an effective amount (measured in g Al/Ha) of one or more of the aforementioned herbicide to the domestic rice crop plant, post-emergence; thereby creating a treated rice plant; and growing the resulting treated rice plant.