The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

A mobile light exposure device or improving the yield and quality of biological material has a first module for emitting one or more light pulses, which includes a light treatment panel whose surface area is between 0.01 m.sup.2 and 10 m.sup.2, a second module or adjusting the optical power density of the treatment panel and optionally adjusting the temperature of the panel, and a means of locomotion for moving the device at a speed of between 1 and 10 km/h. The optical power density of the panel is between 100 W/m.sup.2 and 10,000 W/m.sup.2, preferably between 300 W/m.sup.2 and 3,000 W/m.sup.2, and the light pulses delivered to the biological material are of identical or different wavelengths and of durations less than or equal to two seconds.

The disclosure provides a method of screening tobacco germplasm for resistance to Alternaria alternata by ripening seedling leaves. The method includes ripening seedling leaves, spray inoculation, disease induction, and evaluation of disease resistance. Dense planting, fertilizer control, and potassium increment were used to forcibly ripening seedling leaves. A hospitable environment was simulated to induce disease in the ripened leaves. These treatments reduce differences in leaf maturity and avoid environmental changes. The technique of the disclosure provides greater accuracy and repeatability than the current technique of screening brown spot resistance, and offers the advantages of simple operation, reduced cost, space requirement, and labor intensity, high selection efficiency, and an accurate screening of tobacco phenotypes with resistance to brown spot, etc., used for large-scale screening of tobacco varieties with resistance to brown spot.

The disclosure provides a method of screening tobacco germplasm for resistance to Alternaria alternata by ripening seedling leaves. The method includes ripening seedling leaves, spray inoculation, disease induction, and evaluation of disease resistance. Dense planting, fertilizer control, and potassium increment were used to forcibly ripening seedling leaves. A hospitable environment was simulated to induce disease in the ripened leaves. These treatments reduce differences in leaf maturity and avoid environmental changes. The technique of the disclosure provides greater accuracy and repeatability than the current technique of screening brown spot resistance, and offers the advantages of simple operation, reduced cost, space requirement, and labor intensity, high selection efficiency, and an accurate screening of tobacco phenotypes with resistance to brown spot, etc., used for large-scale screening of tobacco varieties with resistance to brown spot.

The present disclosure discloses a tobacco leaf foliar spraying substance for reducing harmful ingredients in cheroot, a method for reducing carcinogenic components in flue-cured tobacco leaves and flue-cured tobacco shreds. The tobacco leaf foliar spraying substance contains a lotus leaf extract, the harmful chemical ingredients comprise N-nitrosonornicotine, 4-(N-methyl-nitrosamine)-1-(3-pyridinyl)-1-butanone, N-nitrosoanabasine and N-nitrosoanatabine. Foliar spraying is performed on a fertile field by using the lotus leaf extract before tobacco leaves are harvested and modulated, which not only significantly promotesagronomic characters and economic traits of tobacco and alleviates tobacco leaf browning but also effectively reduces harmful chemical ingredients unique to tobacco leaves, such as nitrosamine and nicotine.

The present disclosure discloses a tobacco leaf foliar spraying substance for reducing harmful ingredients in cheroot, a method for reducing carcinogenic components in flue-cured tobacco leaves and flue-cured tobacco shreds. The tobacco leaf foliar spraying substance contains a lotus leaf extract, the harmful chemical ingredients comprise N-nitrosonornicotine, 4-(N-methyl-nitrosamine)-1-(3-pyridinyl)-1-butanone, N-nitrosoanabasine and N-nitrosoanatabine. Foliar spraying is performed on a fertile field by using the lotus leaf extract before tobacco leaves are harvested and modulated, which not only significantly promotesagronomic characters and economic traits of tobacco and alleviates tobacco leaf browning but also effectively reduces harmful chemical ingredients unique to tobacco leaves, such as nitrosamine and nicotine.

The disclosure provides a method of screening tobacco germplasm for resistance to Alternaria alternata by ripening seedling leaves. The method includes ripening seedling leaves, spray inoculation, disease induction, and evaluation of disease resistance. Dense planting, fertilizer control, and potassium increment were used to forcibly ripening seedling leaves. A hospitable environment was simulated to induce disease in the ripened leaves. These treatments reduce differences in leaf maturity and avoid environmental changes. The technique of the disclosure provides greater accuracy and repeatability than the current technique of screening brown spot resistance, and offers the advantages of simple operation, reduced cost, space requirement, and labor intensity, high selection efficiency, and an accurate screening of tobacco phenotypes with resistance to brown spot, etc., used for large-scale screening of tobacco varieties with resistance to brown spot.

The disclosure provides a method of screening tobacco germplasm for resistance to Alternaria alternata by ripening seedling leaves. The method includes ripening seedling leaves, spray inoculation, disease induction, and evaluation of disease resistance. Dense planting, fertilizer control, and potassium increment were used to forcibly ripening seedling leaves. A hospitable environment was simulated to induce disease in the ripened leaves. These treatments reduce differences in leaf maturity and avoid environmental changes. The technique of the disclosure provides greater accuracy and repeatability than the current technique of screening brown spot resistance, and offers the advantages of simple operation, reduced cost, space requirement, and labor intensity, high selection efficiency, and an accurate screening of tobacco phenotypes with resistance to brown spot, etc., used for large-scale screening of tobacco varieties with resistance to brown spot.

Disclosed are methods of improving germination rates of plants/crops, as well as preventing or arresting water evaporation loss from targeted soil areas, which allows for improved water usage efficiency by crops, plants, grasses, vegetation, etc.