A method for organically planting Dendrobium, including using a planting medium in the epiphytic planting step of Dendrobium. The raw materials of planting medium include fermented powder of Dendrobium candidum leaves and sawdust; a method for preparing the fermented powder of Dendrobium candidum leaves is as follows: cutting Dendrobium candidum leaves into small pieces and mashing the small pieces of Dendrobium candidum leaves to obtain materials to be fermented, and then adding liquid medium to the materials to be fermented to obtain fermentation system; sterilizing the fermentation system, adding fermentation bacteria to the fermentation system to obtain a mixture, fermenting and filtering the mixture to obtain post-culturing fermentation broth and fermented products of Dendrobium candidum leaves, dewatering and crushing the fermented products of Dendrobium candidum leaves to obtain fermented powder of Dendrobium candidum leaves.

A method for culturing ginseng cell with high content of ginsenoside, including inducing ginseng cell line: after disinfected and sliced, ultrasonically treating old mountain ginseng, and culturing the old mountain ginseng in a culture medium; screening the ginseng cell line: choosing a variety of culture mediums and using hormones for cell separation and culture, selecting cell lines with better growth morphology and faster growth, and performing solid subculture and liquid suspension culture; optimizing conversion conditions: using acids to treat the chosen cell lines, and controlling the treatment temperature and treatment time, detecting ginsenosides Rg3 and Rh2 in the dried products, determining an optimal treatment condition according to the highest total amount; large-scale industrial production: according to the optimal treatment condition, performing the liquid suspension culture of the selected cell lines and scaling up the scale of culture to obtain large-scale industrial production of ginseng cell products.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

The invention discloses a medium combination for a rapid propagation medium for blueberry stein segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes ½ MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stein segment tissue, the propagation rate of this method is rapid.

A device for directionally placing a plant propagule (2) inside a hollow member (3), as exemplarily illustrated in FIGS. 4-7, comprising a platform element (20) arranged such that a foldable member (1) can be placed thereon; an actuating dispensing arrangement (90a, 90b, 100) for placing a foldable member (1) on the platform element (20); an arrangement (40)/(40a)for placing a plant propagule (2) on a foldable member (1) placed on the platform element (20); optionally, means (150) for identifying an imaginary line (9) on the foldable member (1) stretching through the root forming end (7) to the shoot forming end (8) of the propagule (2) being directionally placed during operation; an actuating folding arrangement (30, 80)/(120/80) for folding the foldable member (1) along said imaginary line (9) to form a folded foldable member (1a); an actuating dispensing arrangement (180) for providing a hollow member (3) having a first open end (4); and an actuating placing arrangement (30, 80)/(120/80) for placing said folded foldable member (1a) into the hollow member (3) through the first open end (4). Related methods for handling plant propagules, in particular somatic plant embryos.

Systems for plant culture include a chamber featuring one or more walls enclosing a spatial volume internal to the chamber, where the one or more walls include a surface for supporting a plant within the enclosed spatial volume, a gas delivery apparatus with at least one gas source, a nutrient delivery apparatus with a reservoir, a sampling apparatus connected to a port formed in the one or more walls, and a controller configured so that during operation of the system, the controller activates the nutrient delivery apparatus to deliver an aqueous growth medium to the plant, and activates the gas delivery apparatus to deliver into the enclosed spatial volume a mixture of isotopically-substituted gases. Also provided are methods of use of the system for measuring nitrogen in a plant and for identifying microbes capable of providing fixed nitrogen to a plant.

Compositions, kits, and methods for preparing a plant cell composition are provided. Compositions, kits, and methods for engineering plant cells are also provided. In some cases, compositions, kits, and methods provided herein can be used to produce cotton.

An apparatus, system and method for removing and treating contaminated materials on a bottom of a body of water and introducing growth packets to revitalize the treated bottom of the body of water. The structure may comprise a vessel with an open face. The vessel may be lowered down to the bottom of the body of water with the face facing down. As a result, the vessel and the bottom form an isolated space. The structure may comprise at least one agitating device(s) for stirring up the materials inside the vessel so as to form a mixture containing the sediment materials which in turn contain the contaminants. Multiple at least one pipe(s) may be coupled to the vessel for transporting the mixture out of the vessel for processing (filtering, treating with chemicals, etc.) so as to neutralize or eliminate the contaminants in the mixture. Then, the treated mixture can be returned to the inside of the vessel via the at least one pipe(s).

A temporary immersion bioreactor (10) for in vitro production of differentiated plant biomass including a growth chamber (12) having one or more transparent side walls (14) and a mesh bottom (16), the mesh bottom (16) defining a plurality of pores (18) to receive plant material. The bioreactor (10) includes a flexible bag (20) formed from a transparent material, the flexible bag (20) having a sealable opening and being dimensioned to receive the growth chamber (12) together with a liquid medium (22). The bioreactor (10) also includes an outer chamber (24) having one or more transparent side walls (26). the outer chamber (24) is formed to correspond in shape to the growth chamber (12) and dimensioned to receive the growth chamber (12) within the flexible bag (20) so that the mesh bottom (16) of the growth chamber (12) faces a bottom (28) of the outer chamber (24) that in use is intended to rest on a support surface. Movement of the growth chamber (12) within the outer chamber (24) is restrained to movement along a single axis such that the mesh bottom (16) of the growth chamber (12) moves towards and away from the bottom (28) of the outer chamber (24).