Methods of producing sugar cane transplant units that includes planting a sugar cane propagation material in a planting container that has a volume of 10 to 200 cubic centimeters; growing the sugar plant to an age of at least 4 months; harvesting the stalks of the sugar cane plant when the stalks have a length of 10 to 50 centimeters; cutting the harvested stalks into stalk segments, wherein the stalk segments are cut to a length of 1 to 5 centimeters; and planting one or more of the stalk segments into a planting container that has a volume from 10 to 200 cubic centimeters.

Grafted cannabis plants and related methods for producing a grafted cannabis plant are provided. In some embodiments, a scion is provided from a first cannabis plant having at least one of a first desired phenotype and a first desired genotype and a rootstock is provided from a second cannabis plant having at least one of a second desired phenotype and a second desired genotype, the rootstock compatible with the scion. The scion may be grafted onto the rootstock to form the grafted plant. In some embodiments, the scion is from a drug-type cannabis plant and the rootstock is from a hemp plant. The grafted cannabis plant may have at least one enhanced plant trait, enhanced harvest trait, and/or emergent property. Related cannabis plant materials and cannabis-derived products are also provided.

The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

Methods and compositions are provided for the creation of a Cas endonuclease-mediated double-strand break and stable integration of a heterologous polynucleotide in the genome of a somatic plant cell, for example in leaf tissue.

Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

The present disclosure relates generally to targeted gene editing constructs, including methods of designing a DNA-recognition moiety for modulation of gene expression in plants, DNA-recognition moieties, gene editing constructs, methods for the modulation of gene expression in plants using gene editing constructs, and plants or regenerable plant cells produced therefrom.

An Agrobacterium-mediated genetic transformation method for sea barleygrass is provided. The method includes: S1, selecting immature embryo materials of sea barleygrass with immature embryos each having a length in a range of 0.5-1.0, sterilizing them with alcohol and sodium hypochlorite to obtain sterilized seeds; S2, separating the immature embryos, crosscutting the immature embryos, and inducing callus generation and proliferation; S3, adjusting pre-culture time and Agrobacterium infection time of calli based on the callus generation and Agrobacterium growth to thereby prevent excessive Agrobacterium liquid; and S4, performing adventitious bud induction culture and rooting induction culture under a shielding-formed low-light environment to obtain tissue culture plantlets. It relates to a tissue culture method for immature embryos of sea barleygrass with high green spot differentiation and plantlet formation rates, which is not limited by materials. A transformation and regeneration system has high genetic transformation and mutation efficiency.

The invention provides novel methods for microspore embryogenesis and the production of doubled haploid embryos. For example, the methods provided include obtaining a plurality of flower buds from a donor plant, determining the developmental stage of microspores comprised within said flower buds, selecting flower buds comprising microspores at a desired developmental stage, treating said flower buds, isolating microspores from said flower buds, and culturing said microspores in induction medium or treating said isolated microspores with a chromosome doubling agent.