The present invention relates to a new in vitro method for direct regeneration of a Cannabis sativa L. plant as well as the use of such method for micropropagation of selected elite clones belonging to Cannabis sativa L., development of polyploid Cannabis plants with enhanced levels of secondary metabolites, promotion of spontaneous rooting of in vitro regenerants in a shorter period of time than conventionally used methods, and production of mutagenized and transgenic or gene-edited Cannabis plants. The method advantageously comprises culturing selected explants lacking already developed meristems such as cotyledons, hypocotyls and/or epicotyls from Cannabis seedlings of short and neutral-day varieties in an appropriate culture medium without plant growth regulators nor chemical microtubule disruptors with a high toxicity grade.

The problem to be solved by this invention is to provide a method of gene introduction for a genus Sorghum plant and a method for producing a transformed genus Sorghum plant with a higher efficiency than the conventionally known Agrobacterium method. This invention provides a gene introduction method of a genus Sorghum plant characterized by using a medium with an increased concentration of a nitrogen source and/or an increased concentration of inorganic ions selected from magnesium ion, potassium ion, calcium ion and sodium ion, in a step of preparing a plant tissue material of a genus Sorghum plant, a step of inoculation with Agrobacterium and/or a co-cultivation step. This invention also provides a method for producing a transformed plant using the gene introduction method of this invention.

The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus formation and regeneration of plants from callus tissue using a histone deacetylase inhibitor.

A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method of comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimized depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

Methods of in vitro clonal propagation, regeneration and transformation in Cannabis are provided. Also provided is the use of such methods in improvements of cannabis cultivars such as via breeding.

Disclosed herein are methods for the production of Cannabis meristem explants from dry seeds. Also described are methods of transforming and gene editing using the Cannabis meristem explants disclosed herein.

The present invention provides a longitudinally split immature tiller separated from a rhizome (LSITR), a cluster of multiple in vitro shoots (CMIT), a cluster of stem segments containing shoot primordia (CSSSP) and an in vitro tiller of Miscanthus x giganteus (Giant miscanthus), and their uses in propagating Miscanthus x giganteus (Giant miscanthus).

A method for culturing ginseng cell with high content of ginsenoside, including inducing ginseng cell line: after disinfected and sliced, ultrasonically treating old mountain ginseng, and culturing the old mountain ginseng in a culture medium; screening the ginseng cell line: choosing a variety of culture mediums and using hormones for cell separation and culture, selecting cell lines with better growth morphology and faster growth, and performing solid subculture and liquid suspension culture; optimizing conversion conditions: using acids to treat the chosen cell lines, and controlling the treatment temperature and treatment time, detecting ginsenosides Rg3 and Rh2 in the dried products, determining an optimal treatment condition according to the highest total amount; large-scale industrial production: according to the optimal treatment condition, performing the liquid suspension culture of the selected cell lines and scaling up the scale of culture to obtain large-scale industrial production of ginseng cell products.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

A hybrid mint plant characterized by an essential oil composition profile, methods of cultivating the hybrid mint plant, and methods of producing an essential oil composition with the essential oil composition profile using the hybrid mint plant are disclosed.