The invention provides seeds and plants of broccoli hybrid SVBL0308. The invention thus relates to the plants, seeds and tissue cultures of broccoli hybrid SVBL0308 and to methods for producing a broccoli plant produced by crossing such plants with themselves or with another plant, such as a broccoli plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, and tissue cultures of broccoli hybrid SVBL0308 comprising introduced beneficial or desirable traits.

The present disclosure provides Brassica oleracea plants having curds or heads exhibiting increased resistance to downy mildew. Such plants may comprise novel introgressed genomic regions associated with disease resistance from Brassica oleracea MYCOCLP. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

The present invention provides a new broccoli variety. The broccoli plant of the present invention includes a broccoli plant identified by Accession No. FERM BP-22393 or a progeny line thereof.

NWB-CMS Brassica oleracea has cytoplasmic male sterility. It relates to NWB-CMS cabbage plant having cytoplasmic male sterility derived from NWB-CMS cabbage line produced by fusion between protoplast of NWB-CMS radish line plant derived from callus of NWB-CMS radish line having cytoplasmic male sterility with inactivated nucleus and protoplast of male fertile cabbage plant with inactivated cytoplasm and a seed thereof, a plant of NWB-CMS Brassica oleracea line having cytoplasmic male sterility produced by breeding of a male fertile Brassica oleracea as a subject for introduction with the NWB-CMS cabbage plant as a breeding line and a seed thereof, a method for producing a hybrid seed of NWB-CMS Brassica oleracea line having cytoplasmic male sterility comprising breeding of a male fertile Brassica oleracea as a subject for introduction with the NWB-CMS cabbage plant as a breeding line, and a hybrid seed of NWB-CMS Brassica oleracea line produced by the method.

A Brassica oleracea plant is provided having self-compatibility, or a progeny thereof, wherein the plant excludes cauliflower and Chinese kale. For example, a Brassica oleracea plant having self-compatibility is provided. Thereby, technical means that makes it possible to accomplish stable and efficient production of parental line seeds for a Brassica oleracea plant can be provided.

Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

The present invention provides a new broccoli variety. The broccoli plant of the present invention includes a broccoli plant identified by Accession No. FERM BP-22393 or a progeny line thereof.

The present application discloses a cabbage having resistance against downy mildew or its progeny. The present application further discloses a method for breeding downy mildew resistant cabbage, including introducing downy mildew resistance from a Brassica oleracea plant having resistance against downy mildew into desired cabbage. One embodiment of the present invention provides a novel cabbage line showing high resistance against downy mildew and having a high commercial value as cabbage, and enables breeding such cabbage.