The unique annual herbaceous Cannabis plant variety ‘ESB-2’ is provided. The variety can be distinguished by its outstanding features of a THCV cannabinoid content ratio of about 2 parts THC to 1 part THCV and a purple leaf stem, when grown under certain conditions.

The unique annual herbaceous Cannabis plant variety ‘ESB-2’ is provided. The variety can be distinguished by its outstanding features of a THCV cannabinoid content ratio of about 2 parts THC to 1 part THCV and a purple leaf stem, when grown under certain conditions.

The present invention relates to a new in vitro method for direct regeneration of a Cannabis sativa L. plant as well as the use of such method for micropropagation of selected elite clones belonging to Cannabis sativa L., development of polyploid Cannabis plants with enhanced levels of secondary metabolites, promotion of spontaneous rooting of in vitro regenerants in a shorter period of time than conventionally used methods, and production of mutagenized and transgenic or gene-edited Cannabis plants. The method advantageously comprises culturing selected explants lacking already developed meristems such as cotyledons, hypocotyls and/or epicotyls from Cannabis seedlings of short and neutral-day varieties in an appropriate culture medium without plant growth regulators nor chemical microtubule disruptors with a high toxicity grade.

The present invention relates to a new in vitro method for direct regeneration of a Cannabis sativa L. plant as well as the use of such method for micropropagation of selected elite clones belonging to Cannabis sativa L., development of polyploid Cannabis plants with enhanced levels of secondary metabolites, promotion of spontaneous rooting of in vitro regenerants in a shorter period of time than conventionally used methods, and production of mutagenized and transgenic or gene-edited Cannabis plants. The method advantageously comprises culturing selected explants lacking already developed meristems such as cotyledons, hypocotyls and/or epicotyls from Cannabis seedlings of short and neutral-day varieties in an appropriate culture medium without plant growth regulators nor chemical microtubule disruptors with a high toxicity grade.

The present disclosure relates generally to methods useful to the production of cannabis plants, including methods for determining the sex of a cannabis plant, methods for determining the developmental stage of a female cannabis plant inflorescence, methods for monitoring the development of female cannabis plant inflorescence, methods for standardising the harvesting of female cannabis plants, methods for selecting a female cannabis plant for harvest and methods for selecting a hypoallergenic cannabis plant.

The present disclosure relates generally to methods useful to the production of cannabis plants, including methods for determining the sex of a cannabis plant, methods for determining the developmental stage of a female cannabis plant inflorescence, methods for monitoring the development of female cannabis plant inflorescence, methods for standardising the harvesting of female cannabis plants, methods for selecting a female cannabis plant for harvest and methods for selecting a hypoallergenic cannabis plant.

The invention generally relates to methods of screening a sample for cannabinoids.

The invention generally relates to methods of screening a sample for cannabinoids.

This technology relates in part to methods of identifying plant cultivars based on the terpene synthase genes that are identified and/or quantified (e.g., copy number, ploidy) in the cultivars. The methods provided herein permit plant cultivars with desired characteristics/phenotypes, e.g., a desired terpene production profile, to be selected for use in various applications, such as agriculture (e.g., selecting cultivars for breeding desired characteristics and/or lineages) and medicine (e.g., therapeutic activity).

The present invention provides a new and distinct Cannabis sativa cultivar designated as ‘UNIQUE FLOWER ORIGINAL HAZE’. This new cultivar is characterized by a persistent green phenotype, extreme resin production, flowering at 14-16 weeks, and significant resistance to pests and fungal growth. ‘UNIQUE FLOWER ORIGINAL HAZE’ is characterized by high levels of Myrcene and Ocimene 2, moderate levels of cis-β Farnesene and β Caryophyllene, and lower levels of additional terpenes including pinenes, α-humulene, linalool, and limonine. ‘UNIQUE FLOWER ORIGINAL HAZE’ has on average 17.38% Total THC and 0.1% Total CBD by dry weight.