The disclosure provides a method of generating a sterile fish, crustacean, or mollusk. The method comprises breeding (i) a fertile hemizygous mutated female fish, crustacean, or mollusk with (ii) a fertile hemizygous mutated male fish, crustacean, or mollusk, selecting a female progenitor that is homozygous by genotypic selection, and breeding the homozygous female progenitor to produce the sterile fish, crustacean, or mollusk. The mutation disrupts the maternal-effect of a primordial germ cell (PGC) development gene and does not impair the viability, sex determination, fertility, or a combination thereof, of a homozygous progenitor. The disclosure also provides methods of making broodstock freshwater and seawater organisms for use in producing sterilized freshwater and seawater organisms, as well as the broodstock itself.

The present invention provides a breeding method of a new variety of hard-shelled razor clams, comprising the following steps: a. Estimating the heritability of shell hardness trait: constructing full-sib families to estimate the heritability of the growth and shell hardness traits of the razor clams; b. Selecting individuals with hard shells from basic population using an electronic hardness tester of material mechanics as the broodstock clams; c. Artificially-induced spawning; d. The offspring grow-out; e. Purification of the new strain: with the new strain of hard-shelled generation 1 adults obtained in step d as broodstocks, repeating the steps c and d several times to obtain a new variety of razor clams with hard shells, and the shell hardness trait of razor clams has stable heritability.

The invention relates to a cultured bivalve mollusk (particularly quahog) pearl and a method of preparation thereof. The method includes implanting a nucleus material and a donor bivalve mollusk mantle tissue in the gonad or gut area of an anesthetized bivalve mollusk. An ideal bivalve mollusk or a quahog is four-years old or younger for rapid pearl production. The cultured pearl is harvested after a period ranging from six months to three years. Such cultured bivalve mollusk pearl is identical in appearance to the natural pearls.

Improved molluscan shellfish in diploid, tetraploid and triploid forms are provided. Also provided are methods for improving molluscan shellfish through progressive rotational crossbreeding and/or coalesced interploidy breeding.

The present invention provides systems and non-invasive methods for gender selection of eukaryotic organisms More specifically, the invention applies the CRISPR-Cas system as well as any derivatives and fusion proteins thereof for creation of transgenic eukaryotic organisms and for selecting the desired gender of the resulting progeny.

The present disclosure describes systems and methods for producing triploid shellfish.

The invention relates to a cultured bivalve mollusk (particularly quahog) pearl and a method of preparation thereof. The method includes implanting a nucleus material and a donor bivalve mollusk mantle tissue in the gonad or gut area of an anesthetized bivalve mollusk. An ideal bivalve mollusk or a quahog is four-years old or younger for rapid pearl production. The cultured pearl is harvested after a period ranging from six months to three years. Such cultured bivalve mollusk pearl is identical in appearance to the natural pearls.

Improved molluscan shellfish in diploid, tetraploid and triploid forms are provided. Also provided are methods for improving molluscan shellfish through progressive rotational crossbreeding and/or coalesced interploidy breeding.

This cephalopod breeding method is a method for breeding cephalopod larvae in a rearing tank having a water changing means. The method includes a water-changing period during which the ratio of water changed by the water-changing means per day is 200% or more relative to the quantity of water in the rearing tank in a floating breeding period of the cephalopod larvae.