The present disclosure provides a recirculating aquaculture system comprising a) a wastewater treatment system and b) a polyhydroxyalkanoate (PHA) production system. In particular, poly(3-hydroxybutyrate) (PHB) can be utilized as the PHA in such a system. Methods of treating wastewater for reuse by contacting the wastewater with one or more zeolites to remove material from the wastewater and reusing the treated wastewater are also provided as well as methods of producing a PHA such as PHB from organic waste. Moreover, food compositions comprising a PHA biomass as well as associated methods are also provided.

The invention relates to a method for producing a solid transformation product of a substrate comprising the following steps: •preparing a substrate of biomass comprising carbohydrates and proteinaceous matter that originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with carbohydrates and proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, •mixing said substrate with a live microorganism or a combination of live microorganisms, which live microorganism or mixture of live microorganisms is not live yeast, and adding water in an amount which provides an initial incubation mixture having a water content from 30 to 70% by weight, and a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; •incubating said initial incubation mixture for 1-240 hours at a temperature of 15-70° C.; and thereafter recovering wet solid transformation product from the incubation mixture; further comprising that the incubating step is performed as a continuous plug-flow process in a vertical, non-agitated incubation tank with inlet means for said mixture and additives and outlet means for said solid transformation product.

The invention relates to a method for producing a solid transformation product of a substrate comprising the following steps: •preparing a substrate of biomass comprising carbohydrates and proteinaceous matter that originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with carbohydrates and proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, •mixing said substrate with a live microorganism or a combination of live microorganisms, which live microorganism or mixture of live microorganisms is not live yeast, and adding water in an amount which provides an initial incubation mixture having a water content from 30 to 70% by weight, and a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; •incubating said initial incubation mixture for 1-240 hours at a temperature of 15-70° C.; and thereafter recovering wet solid transformation product from the incubation mixture; further comprising that the incubating step is performed as a continuous plug-flow process in a vertical, non-agitated incubation tank with inlet means for said mixture and additives and outlet means for said solid transformation product.

The disclosure discloses Lactobacillus sp. BB1 enhancing memory ability, a fermented food and application thereof, and belongs to the technical field of microorganisms. The Lactobacillus sp. BB1 disclosed by the disclosure can be used for preparing functional foods, health-care products and drugs that enhance the memory ability of a healthy individual and improve memory impairment. The disclosure further provides the fermented food. The fermented food is prepared by fermentation with Lactobacillus sp. BB1, comprises a solid food, a liquid food and a semisolid food, and has a wide application prospect.

A functional feed additive which may promote metabolism in livestock without the use of separate antibiotics, antibacterial agents and growth hormones, thereby increasing autoimmune cells to maximize the autoimmunity of livestock against various diseases, and which makes it possible to obtain meat containing increased amounts of unsaturated fatty acids beneficial to the human body and which has an effect of significantly reducing the complex odor of livestock excrement by removing ammonia gas (NH.sub.3) generated from livestock excrement.

Provided is a method for the manufacture of a butyrate product by fermentation of a carbon source-containing fermentation feedstock with a Clostridia class bacterium which natively produces butyric acid while maintaining a pH of about 5 to 7, wherein ammonia is used for said maintaining pH and whereby a fermentation broth comprising ammonium butyrate, a biomass, and optionally a fermentation by-product is formed; separating said biomass from said fermentation broth to form separated biomass and a clarified fermentation broth, wherein said clarified fermentation broth comprises said ammonium butyrate and optionally said fermentation by-product; and adding to said clarified broth an additive selected from the group consisting of a mineral acid, a mineral base, a soluble calcium salt and combinations thereof, whereby said butyrate product is formed in said clarified broth. Further provided are feed ingredients, animal feeds and anti-icing agents comprising the composition, and uses thereof.

Provided is a method for the manufacture of a butyrate product by fermentation of a carbon source-containing fermentation feedstock with a Clostridia class bacterium which natively produces butyric acid while maintaining a pH of about 5 to 7, wherein ammonia is used for said maintaining pH and whereby a fermentation broth comprising ammonium butyrate, a biomass, and optionally a fermentation by-product is formed; separating said biomass from said fermentation broth to form separated biomass and a clarified fermentation broth, wherein said clarified fermentation broth comprises said ammonium butyrate and optionally said fermentation by-product; and adding to said clarified broth an additive selected from the group consisting of a mineral acid, a mineral base, a soluble calcium salt and combinations thereof, whereby said butyrate product is formed in said clarified broth. Further provided are feed ingredients, animal feeds and anti-icing agents comprising the composition, and uses thereof.

The present invention relates to the technical field of foods and additives that are derived from edible plants. Particularly, the present invention relates to a method for obtaining a product rich in antioxidants, dietary fiber and other nutrients from byproducts of edible plants such as dehydrated pulps and juices derived from fruits and vegetables, obtaining thus a first liquid product (first liquid phase), a second liquid product (second liquid phase), and a first and second solid phases, all of them with multiple beneficial health properties. One of the main effects observed when an individual consumes these products, is a normalization in the levels of triglycerides, total cholesterol, and HDL, and the body weight of this individual is maintained even when under a regime of fat diet.

Provided are methods of making and using an antimicrobial composition, optionally including adding one or more enzymes to a suspension of lees, wherein the lees was formed by fermenting fruit with yeast and the one or more optional enzymes comprise a protease, a carbohydrase, or a combination of a protease and a carbohydrase; and forming a dried lees by drying the lees. In some examples the fruit includes chardonnay grapes, pinot noir grapes, cabernet franc grapes, or a combination of any two or more of chardonnay grapes, pinot noir grapes, and cabernet franc grapes; the yeast includes one or more Saccharomyces cerevisiae strains of yeast; and drying the lees by heating it. In some examples, trub made from fermenting a grain such as barley is used instead of lees. An antimicrobial composition made as provided may be administered to an animal to inhibit bacterial growth.

Provided are methods of making and using an antimicrobial composition, optionally including adding one or more enzymes to a suspension of lees, wherein the lees was formed by fermenting fruit with yeast and the one or more optional enzymes comprise a protease, a carbohydrase, or a combination of a protease and a carbohydrase; and forming a dried lees by drying the lees. In some examples the fruit includes chardonnay grapes, pinot noir grapes, cabernet franc grapes, or a combination of any two or more of chardonnay grapes, pinot noir grapes, and cabernet franc grapes; the yeast includes one or more Saccharomyces cerevisiae strains of yeast; and drying the lees by heating it. In some examples, trub made from fermenting a grain such as barley is used instead of lees. An antimicrobial composition made as provided may be administered to an animal to inhibit bacterial growth.