The present invention provides a reporter system comprising (i) a gene expression construct for expression in a cell of a reporter gene, said reporter gene encoding a fusion protein comprising a transmembrane domain fused in-frame to a reporter domain, wherein said transmembrane domain upon insertion of the fusion protein into the cell membrane anchors the fusion protein in the cell membrane while expressing the reporter domain at the cell surface, and (ii) a reporter peptide labeled with a radiolabel, wherein said reporter domain comprises the large polypeptide subunit of a split luciferase, and wherein said reporter peptide comprises the small peptide subunit of said split luciferase, wherein both subunits associate by complementation to assemble into a luciferase complex.

A computing device for diffusion dictionary imaging (DDI) of microstructure and inflammation of a patient is provided. The DDI computing device is connected to other computing devices, such as a magnetic resonance imaging (MRI) scanner. The DDI computing device receives magnetic resonance (MR) signals from the MRI scanner. Once received, the DDI computing device records the one or more MR signals to a memory device. The DDI computing device computationally processes the one or more MR signals to reconstruct a diffusion MRI image using diffusion dictionary data. The MR signals include values that are used as input to algorithms of the DDI data to reconstruct the diffusion MRI image. A database is used to store DDI data, artificial intelligence (AI) data, diffusion dictionary data, and MR data.

Systems and methods are provided for performing a medical procedure within a patient's body that involves a thoracic duct including an ostium communicating with the patient's venous system. A distal end of a catheter is introduced through the patient's venous system into a body lumen adjacent the ostium of the thoracic duct. An expandable member on the distal end of the tubular member may be expanded adjacent the ostium, e.g., within the body lumen or the thoracic duct itself, and used to isolate the thoracic duct from the body lumen, whereupon a medical procedure may be performed via the thoracic duct. For example, lymphatic fluid may be removed from the thoracic duct through a lumen of the tubular member and/or one or more agents may be introduced into the thoracic duct through the tubular member.

A method of assessing the brain lymphatic or glymphatic system and the glucose transporter function on blood-cerebrospinal fluid barrier (BCSFB) of a subject using D-glucose or a D-glucose analog. A spatial map is generated of water MR signals that are sensitized to changes in D-glucose or a D-glucose analog in cerebrospinal fluid (CSF) of the subject. The spatial map is observed at one or more time points before, one or more time points during, and one or more time points after, raising the blood level of the D-glucose or a D-glucose analog in the subject CSF. A difference is detected between the MR signals of the spatial map before, during, and after raising the blood level of D-glucose or a D-glucose analog. A physiological parameter associated with the brain lymphatic or glymphatic system and the glucose transporter function on BCSFB of the subject is ascertained based on the detected difference.

A method of imaging tissue of a subject using an electronic rolling shutter imager includes sequentially resetting rows of pixels of the rolling shutter imager from a first row to a last row, sequentially reading charge accumulated at the rows of pixels from the first row to the last row, wherein the first row is read after resetting the last row, illuminating the tissue of the subject with illumination light for an illumination period that lasts longer than a vertical blanking period, wherein the vertical blanking period is the period from the resetting of the last row to the reading of the first row, and generating an image frame from the readings of charge accumulated at the rows of pixels, wherein at least one reading of charge accumulated at a row of pixels is removed or replaced to generate the image frame.

Apparatuses and methods for analyzing fluid samples are provided. For example, an example apparatus may include a fluid imaging chamber, at least one illumination source component, and an image sensor component. In some examples, the fluid imaging chamber comprises a flow channel for receiving a fluid sample. In some examples, the at least one illumination source component is configured to emit at least one light beam, and the at least one light beam is directed through the fluid sample in the flow channel from a top surface of the fluid imaging chamber. In some embodiments, the image sensor component is positioned under a bottom surface of the fluid imaging chamber and configured to generate digital holography image data of the fluid sample.

According to an embodiment of the present disclosure, there is provided a laser spectroscopy-based independent device, including: a spectrometer configured to measure a spectrum of generated light which is generated by a laser projected onto a sample; and a disease analysis module configured to determine whether there is lesion tissue by applying a lesion tissue detection learning model to a result of non-discrete spectrum measurement, which is measured by the spectrometer, wherein the spectrometer is configured to measure spectra of all generated light that is generated from a time when the laser is projected onto the sample.

Aspects of the invention include methods for visualizing lymphatic tissue in an ocular region, e.g., the cornea, of a living subject, such as a mouse or human. In certain embodiments, the methods include contacting the region with a fluorescently-labeled lymphatic tissue-specific dye, e.g., FITC or rhodamine labeled dextran, and detecting the labeled dye to visualize lymphatic tissue in the region, where the visualization may vary, from an image obtained at a single time to a video over a period of time. The invention finds use in a variety of different applications, including research and therapeutic applications.

In some aspects, the invention provides methods of treating a subject in need of treatment for a chronic complement-mediated disorder. In some aspects, the invention provides methods of treating a subject in need of treatment for a Th17-associated disorder. In some aspects, the invention provides methods of treating a subject in need of treatment for a chronic respiratory system disorder. In some aspects, the invention provides methods of administering a complement inhibitor to a subject. In some embodiments, a method of treating a subject comprises administering multiple doses of a complement inhibitor to the subject according to a dosing schedule that leverages the prolonged effect of complement inhibition in chronic respiratory disorders. In some embodiments, a subject has chronic obstructive pulmonary disease. In some embodiments, a subject has asthma.

A computing device for diffusion dictionary imaging (DDI) of microstructure and inflammation of a patient is provided. The DDI computing device is connected to other computing devices, such as a magnetic resonance imaging (MRI) scanner. The DDI computing device receives magnetic resonance (MR) signals from the MRI scanner. Once received, the DDI computing device records the one or more MR signals to a memory device. The DDI computing device computationally processes the one or more MR signals to reconstruct a diffusion MRI image using diffusion dictionary data. The MR signals include values that are used as input to algorithms of the DDI data to reconstruct the diffusion MRI image. A database is used to store DDI data, artificial intelligence (AI) data, diffusion dictionary data, and MR data.