A non-invasive method and system for assessing the survival of a transplanted flap involve the following steps. A control unit instructs a variable-frequency current generator circuit to generate a constant current at a fixed frequency and pass the constant current through a detection electrode. The detection electrode detects a bioelectrical impedance of the skin to which the flap has been transplanted, and the bioelectrical impedance of the skin is compared with a pre-defined threshold value. If the bioelectrical impedance of the skin exceeds the pre-defined threshold value, it is determined that an abnormal condition has occurred.

An apparatus includes a flexible container and a port. The container includes a first layer coupled to a second layer to define a storage volume within which a tissue specimen can be contained. The first layer has a first stiffness and the second layer has a second stiffness. An edge of the first layer is spaced apart from an edge of the second layer to define an opening into the storage volume. The edges of the first and second layer form a peelable seal that hermetically seals the storage volume such that the first layer can be peeled away from the second layer to expose the storage volume. The port is coupled to the flexible container and allows fluid communication between the storage volume and an external volume.

A method includes forming a scaffold and seeding the scaffold with live cells; growing the cells in the scaffold; and 3D printing the cells into a living subject, where the cells continue to live in the living subject.

Systems, instruments, methods, and compositions are described involving removing a portion of the epidermis within a donor site on a subject, and harvesting dermal plugs within the donor site. An injectable filler is formed by mincing the dermal plugs. The injectable filler is configured for injecting into a recipient site on the subject.

A biomimetic tissue scaffold for repairing an elongated tissue in need of repair can comprise a plurality of coiled flexible polymeric ribbons having a surface on which is formed an array of nanofibers, the ribbons forming a tubular body defining a first open end in which a first end of the elongated tissue is receivable, a second open end in which a second end of the elongated tissue is receivable, and a lumen extending between the first and second open ends.

Provided is an artificial epidermis structure. Three different contact states can be realized between the artificial epidermis structure and a target object. The “first contact state” is a state in which only the surface of a distal end portion of each protrusion contacts the target object. The “second contact state” is a state in which each protrusion tilts with respect to a base body corresponding to the force received from the target object and thereby the surface of a base portion and the surface of the distal end portion of each protrusion contact the target object. The “third contact state” is a state in which each protrusion further tilts with respect to the base body corresponding to an increase in the force and thereby the surface of the base body and respective surface of the distal end portion and the base portion of each protrusion contact the target object.

A compliant scaffold incorporates a plurality of elongated apertures that form a geometric pattern enabling biaxial expansion or contraction. An elongated aperture has a pair of nodes located on opposing sides of the aperture and between a pair of antinodes located on the extended and opposing ends of the elongated aperture. A geometric pattern may have various geometric shapes, or tiles, between the plurality of apertures. The geometric tiles have a bounded perimeter formed by the plurality of elongated apertures. A substantial portion of the elongated apertures may be configured with the antinodes proximal to one of said pair of nodes of a separate elongated aperture; wherein the antinodes are closer to one of the pair of nodes than to any other antinode. This unique arrangement of the elongated apertures may be formed in biological material in vivo or ex vivo.

The present invention provides an artificial skin and a preparation method thereof. The present invention takes the xenogeneic acellular dermal matrix particles as main materials, and obtains the dermis layer by three-dimensional printing technologies, and then obtains the artificial skin by combining the epidermis layer with the dermis layer. The dermis layer of artificial skin in present invention has three-dimensional porous structure, which retains main components of natural dermal matrix in composition, and imitates distributed structure at fiber bundle diameter and pore size of natural dermal matrix in structure. This kind of novel biomimetic dermal scaffolds have obvious advantages in inducing migration and regeneration of skin cells, accelerating vascularization, promoting wound healing and improving healing quality. The dermis layer of artificial skin in present invention is obtained by three-dimensional printing technologies, which has precise and controllable structure, simple preparation method and high products qualification rate.

A bio-printing process comprises a step of preparing a target digital model representative of the three-dimensional organization of the tissue to be produced, a step of controlling a bio-printing instrument for the deposition of a plurality of layers of living cells and of biomaterials, a step of calculation of a digital printing model as a function of the digital model of the product to be produced, and of a model predicting change, and also characteristics of the constituents to be printed. The step of controlling the bio-printing instrument is carried out according to the digital printing model calculated in this way. A system is also described for implementing this process.

A stretchable sensor is provided. The stretchable sensor includes a first stretchable electrode including a first elastomer and a first conductor dispersed in the first elastomer, a stretchable active layer formed on the first stretchable electrode and including a third elastomer and an ion conductor dispersed in the third elastomer, and a second stretchable electrode formed on the stretchable active layer and including a second elastomer and a second conductor dispersed in the second elastomer. The stretchable sensor is effectively capable of sensing a temperature without being affected by strain and recognizing strain without being affected by temperature.