A method to divide liposuction fat into aliquots for use and cryopreservation purposes, the method comprising: providing a taking container that contains adipose material removed by liposuction, the adipose material including fat and aqueous fluid; providing a plurality of cryopreservation containers; taking a quantity of the adipose material from the taking container, keeping the quantity of the adipose material isolated from an external environment; separating by gravity the fat from the aqueous fluid in the adipose material of the taken quantity; and transferring the separated fat into one or more cryopreservation containers, to define isolated aliquots of fat.

A closed-system (i.e., hermetically sealed) methods, and kits for isolating Mesenchymal Stromal Cells (MSCs) from lipoaspirate, that utilizes specific hermetically-sealed, disposable assemblies and containers that are aseptically interconnected, are disclosed. Contemplated methods require lipoaspirate as a starting material, and provide for obtaining isolated, purified MSCs at the end of the process. Kits are contemplated to contain disposable assemblies, composed by sterile components such as modified syringes, tubing, centrifuge tubes, filtering units, that can be aseptically connected while maintaining sterility thereby keeping the system closed (i.e., hermetically sealed) with respect to the external environment. As a result, contamination during the isolation process can be avoided. The MSCs that are obtained at the end of the processing are thus ready to be further manipulated in subsequent operations (in example, expansion) also for therapeutic purposes.

An injector arrangement which allows the fat tissue to be drawn through a suction tip, stored inside a tube without intervention or exposure to air and centrifuged obtain fat based stem cells. The arrangement includes a modular piston with a movable structure which allows drawing the fat tissue into the tube through the suction tip by a piston tip seal, a piston shaft with modular structure which allows the said modular piston to move inside the tube and which can be separated from the modular piston in order to allow the tube to be placed inside the present centrifuge device after the fat tissue is drawn inside the tube, and an O-ring which engages into the O-ring seat formed on the hollow locking part in order to make the hollow locking part introduced on the modular piston to act as a locking device during the centrifuge.

Provided is an apparatus and process for isolation of stromal vascular fraction (SVF) following lipoaspiration.

A fat harvesting systems and related methods of harvesting and reinjecting fat that provides a medical professional with multiple technique options. Harvested fat can be subjected to a variety of pre-reinjection preparation steps within a single, self contained reservoir such that the potential for contaminating the harvested fat is avoided. The fat harvesting system can be completely self-contained kit requiring no additional external systems or alternatively, can utilize available surgical suite systems, for example, vacuum to successfully harvest, prepare and reinject fat cells. The fat harvesting system can size harvested fat cells so as to be especially desirable for different injection locations in the body. The fat harvesting system is a single use, disposable system that requires no sterilization equipment and recovered and sized fat cells are maintained in a sanitary environment so as to avoid contamination that could result in having to discard recovered fat cells.

A method to divide liposuction fat into aliquots for use and cryopreservation purposes, the method comprising: providing a taking container that contains adipose material removed by liposuction, the adipose material including fat and aqueous fluid; providing a plurality of cryopreservation containers; taking a quantity of the adipose material from the taking container, keeping the quantity of the adipose material isolated from an external environment; separating by gravity the fat from the aqueous fluid in the adipose material of the taken quantity; and transferring the separated fat into one or more cryopreservation containers, to define isolated aliquots of fat.

An adipose tissue (AT) transfer system includes, on the aspiration side, an aspiration cannula, an aspiration pump, a container, and flexible tubing connecting the aspiration cannula to the container. On the reinjection side, the system includes a reinjection cannula, flexible tubing connecting the inlet of the reinjection cannula to the container, and a reinjection pump imposing positive-displacement pumping action on the flexible tubing and causing movement of AT in a continuous or pulsed mode. The aspiration pump operates to continually supply harvested AT to the second flexible tubing while the reinjection pumps causes continuous or pulsed deposition of the AT at the injection site. To ensure that internal pressure and/or flow of the AT through a channel of delivery of the AT to the reinjection site does not exceed a predetermined value, defined with respect to pressure causing rupture of a blood-vessel at the recipient site, the system contains an external pressure sensor configured to measure such internal pressure in absence of a part that is in direct contact with the AT.

An adipose tissue (AT) transfer system includes, on the aspiration side, an aspiration cannula, an aspiration pump, a container, and flexible tubing connecting the aspiration cannula to the container. On the reinjection side, the system includes a reinjection cannula, flexible tubing connecting the inlet of the reinjection cannula to the container, and a reinjection pump imposing positive-displacement pumping action on the flexible tubing and causing movement of AT in a continuous or pulsed mode. The aspiration pump operates to continually supply harvested AT to the second flexible tubing while the reinjection pumps causes continuous or pulsed deposition of the AT at the injection site. To ensure that internal pressure and/or flow of the AT through a channel of delivery of the AT to the reinjection site does not exceed a predetermined value, defined with respect to pressure causing rupture of a blood-vessel at the recipient site, the system contains an external pressure sensor configured to measure such internal pressure in absence of a part that is in direct contact with the AT.

Provided is an apparatus and process for isolation of stromal vascular fraction (SVF) following lipoaspiration.

Disclosed herein are devices and methods for processing biologic tissues, such as adipose tissue or whole blood, via centrifugation, An annular cavity is present, typically at the widest interior point of a rotatable chamber. The device is operable such that a sample of biologic tissue present in the rotatable chamber can be stratified into at least two constituent layers as a function of the differing specific gravities of the constituents and at least a portion of one of the constituent layers captured in the annular cavity.