As various embodiments of the present disclosure related to an electronic cell separating apparatus using ultrasonic waves are described, a cell separating apparatus according to an embodiment may include: a fat removal body part; a handpiece including an ultrasonic wave generating unit electrically connected to the fat removal body part; a tip part provided in the handpiece; a temperature sensor unit for detecting the temperature of the tip part, the temperature sensor unit being provided in the tip part; an interlocked pump part for feeding cooling water to the tip part, the interlocked pump part being disposed in the body unit and being connected to the handpiece; and a controller for receiving a signal detected by the temperature sensor unit, and operating the interlocked pump part at a preconfigured temperature. In addition, there may be various other embodiments related to the cell separating apparatus.

A flexible bag for the preparation of an adipose tissue graft provided with a proximal end and a distal end and comprising: a front wall and a rear wall, which are connected to one another to define a receiving space on the inside; a first filter arranged inside the bag to divide the receiving space into a first chamber and a second chamber and to micro-fragment the adipose tissue to obtain a micro-fragmented adipose tissue; and a second filter arranged inside the second chamber to obtain a third chamber and to obtain the graft by separating a waste material from the micro-fragmented adipose tissue. The first filter comprises a distal edge which is connected to the front wall between the proximal end and the distal end and the second filter comprises a proximal edge which is connected to the rear wall between the proximal end and the distal end.

A device and associated method for centrifuging a physiological fluid includes a container including a tip, a base, and a barrel extending between the tip and the base to hold a physiological fluid. The container includes a plunger positioned within the barrel of the container. The plunger includes a concave collection surface that faces the tip of the container. A plunger gasket seal is in sealing engagement with an inside wall of the barrel of the container. A bottom support is provided to support the base of the container at least partially within the bottom support for use in a centrifuge. The container is coupled to the bottom support using an interference fit. The collection surface of the plunger and at least the barrel can be made of the same material. Advantageously, the device can be used to process blood without the need for any added anticoagulant in the blood.

A fat harvesting system and related methods of harvesting and reinjecting fat that provides a medical professional with multiple technique options. Harvested fat can be subjected to a variety of pre-reinjection preparation steps within a single, self-contained reservoir such that the potential for contaminating the harvested fat is avoided. The fat harvesting system can be completely self-contained kit requiring no additional external systems or alternatively, can utilize available surgical suite systems, for example, vacuum to successfully harvest, prepare and reinject fat cells. The fat harvesting system can size harvested fat cells so as to be especially desirable for different injection locations in the body. The fat harvesting system is a single use, disposable system that requires no sterilization equipment and recovered and sized fat cells are maintained in a sanitary environment so as to avoid contamination that could result in having to discard recovered fat cells.

A fat harvesting system and related methods of harvesting and reinjecting fat that provides a medical professional with multiple technique options. Harvested fat can be subjected to a variety of pre-reinjection preparation steps within a single, self-contained reservoir such that the potential for contaminating the harvested fat is avoided. The fat harvesting system can be completely self-contained kit requiring no additional external systems or alternatively, can utilize available surgical suite systems, for example, vacuum to successfully harvest, prepare and reinject fat cells. The fat harvesting system can size harvested fat cells so as to be especially desirable for different injection locations in the body. The fat harvesting system is a single use, disposable system that requires no sterilization equipment and recovered and sized fat cells are maintained in a sanitary environment so as to avoid contamination that could result in having to discard recovered fat cells.

A method of collecting mononuclear cells includes separating whole blood into plasma and cellular components, purifying the plasma through a plasma adsorption column to create purified plasma, combining the cellular components with the purified plasma to form a first mixture, and separating the first mixture into mononuclear cells and at least one component. Alternatively, whole blood may be flowed through an adsorption column to create purified whole blood, with the purified whole blood then being separated into mononuclear cells and at least one component.

A method for skin ligament injection incorporates the benefits of regenerative medicine to provide more natural and longer lasting results in the revitalization of a patient's face. The method includes a first phase in which through the use of aspiration tubes with reduced diameter, small aspiration holes and controlled aspiration pressure with aspiration in a superficial plane of the skin, a user collects tissue rich in stein cells with waste of fibrotic tissue. A second phase includes the sterile manipulation and washing of fat to collect only the tissue containing the stein cells arranged around vascular structures and growth factors collected inside organelles called exosomes. Lastly, a third and final phase includes injecting the obtained product into the skin of a patient.

A screen exchange device for reducing the size of a biological tissue according to one embodiment comprises: a disk comprising a plurality of screens each having at least one through-hole configured to reduce the size of a biological tissue, the screens having different through-hole characteristics; a first cover which covers a first side of the disk and includes a first opening through which a biological tissue passes; a second cover which covers a second side of the disk and includes a second opening through which the biological tissue passes; a housing configured to receive the disk; and a manipulator which selects any one screen among the plurality of screens and manipulates the first cover or the second cover such that the selected screen communicates with the first opening and the second opening.

A fat harvesting systems and related methods of harvesting and reinjecting fat that provides a medical professional with multiple technique options. Harvested fat can be subjected to a variety of pre-reinjection preparation steps within a single, self contained reservoir such that the potential for contaminating the harvested fat is avoided. The fat harvesting system can be completely self-contained kit requiring no additional external systems or alternatively, can utilize available surgical suite systems, for example, vacuum to successfully harvest, prepare and reinject fat cells. The fat harvesting system can size harvested fat cells so as to be especially desirable for different injection locations in the body. The fat harvesting system is a single use, disposable system that requires no sterilization equipment and recovered and sized fat cells are maintained in a sanitary environment so as to avoid contamination that could result in having to discard recovered fat cells.

A method of collecting mononuclear cells includes separating plasma from cellular components of whole blood. The cellular components, which include mononuclear cells and red blood cells, are combined with plasma replacement fluid to form a first mixture. The mononuclear cells of the first mixture are separated from the red blood cells of the first mixture, which may be followed by extracorporeal photopheresis being performed on the mononuclear cells.