Provided herein are methods for the isolation of platelets, for example, isolation of platelets from umbilical cord blood. In certain embodiments, presented herein are methods for preparation of platelet rich plasma. In one aspect, provided herein are methods for isolation of platelets from blood. In certain embodiments, presented herein are methods for isolation of platelets from cord blood, e.g., human cord blood. The isolated platelets can be used for a variety of applications, including, for example, methods of wound healing, organ repair and/or regeneration, and/or tissue repair and/or regeneration, in either autologous or allogenic settings.

Disclosed is a system for the collection, processing and reuse of amniotic fluid and placental aspirate at a C-section site. The system includes a canister positioned along the vacuum line through which the amniotic fluid and placental aspirate is suctioned. The canister has a coil whereby the heavier cellular components, including stem cells, platelets and growth factors, are separated coincident with the surgical procedure. The canister has a port whereby the heavier cellular material can be removed from the canister. The heavier cellular material can be then applied to the wound site of the cesarean section patient. The system disclosed allows for the processing of the amniotic fluid and placental aspirate to take place in the same room as the surgical procedure. A kit and method are also provided.

An apparatus and method for collecting blood from the umbilical cord of a newborn into a standard vacuum collection tube is disclosed wherein the apparatus comprises a vessel in the general shape of a cup with an open top, a receiving cavity and a tapered bottom surface wherein the taper of the tapered bottom surface is away from the receiving cavity; a pick up tube with open top end and closed bottom, and side tabs, a needle inserted through an opening in the closed bottom of said tube wherein the diameter of the opening is of sufficient size to only allow the tip of the needle to be inserted, and a means for providing an air tight seal between the inserted needle and said opening.

Described herein are systems, devices, and methods for an extracorporeal, artificial, placenta. In some embodiments, an artificial placenta and amniotic bed system may comprise a control unit, a gas delivery unit, a gas exchange unit or membrane oxygenator, a fluids delivery unit, an amniotic fluid bed, and a human machine interface. In some embodiments, the artificial placenta and amniotic bed systems, devices, and methods described herein may improve survival rates and minimize long-term disabilities in preterm, gestational-age, newborns. In some embodiments, the extracorporeal systems, devices, and methods comprise an artificial network through which oxygen and nutrient-rich blood may flow into a fetus (residing in an amniotic fluid bed), while carbon dioxide and wastes may be removed, thus re-establishing a form of intrauterine placental circulation.

Devices and methods for extraction and processing of substantia gelatinea funiculi umbilicalis (Wharton's Jelly) from an umbilical cord. Isolated pluripotent cell compositions and methods of using the same are also provided.

Described herein are systems, devices, and methods for an extracorporeal, artificial, placenta. In some embodiments, an artificial placenta and amniotic bed system may comprise a control unit, a gas delivery unit, a gas exchange unit or membrane oxygenator, a fluids delivery unit, an amniotic fluid bed, and a human machine interface. In some embodiments, the artificial placenta and amniotic bed systems, devices, and methods described herein may improve survival rates and minimize long-term disabilities in preterm, gestational-age, newborns. In some embodiments, the extracorporeal systems, devices, and methods comprise an artificial network through which oxygen and nutrient-rich blood may flow into a fetus (residing in an amniotic fluid bed), while carbon dioxide and wastes may be removed, thus re-establishing a form of intrauterine placental circulation.

A system for obtaining plasma enriched in platelets is disclosed which is closed to the atmosphere. The system includes: a collection tube containing an anticoagulant portion and a separation gel; a first collection syringe adapted to collect a portion of fluid relatively depleted in platelets from said collection tube after centrifugation; and a second collection syringe adapted to collect plasma enriched in platelets from said collection tube after centrifugation, said second collection syringe further comprising a filter unit adapted to filter cells included in said plasma enriched in platelets.

Provided herein are methods for the isolation of platelets, for example, isolation of platelets from umbilical cord blood. In certain embodiments, presented herein are methods for preparation of platelet rich plasma. In one aspect, provided herein are methods for isolation of platelets from blood. In certain embodiments, presented herein are methods for isolation of platelets from cord blood, e.g., human cord blood. The isolated platelets can be used for a variety of applications, including, for example, methods of wound healing, organ repair and/or regeneration, and/or tissue repair and/or regeneration, in either autologous or allogeneic settings.

Devices and methods for extraction and processing of substantia gelatinea funiculi umbilicalis (Wharton's Jelly) from an umbilical cord. Isolated pluripotent cell compositions and methods of using the same are also provided.

The present disclosure provides an apparatus, and methods of using same, for collecting blood from an umbilical cord in a sterile environment that includes an umbilical cord retaining assembly and a blood collection assembly rotatably connected to the umbilical cord retaining assembly. A cord cutting blade, which is carried by the blood collection assembly, functions to cut the umbilical cord when the blood collection assembly is rotated relative to the umbilical cord retaining assembly. After the umbilical cord is cut, blood flows by force of gravity into the blood collection region of the blood collection assembly to which a blood collection bag can be interconnected.