A suppressor device for an ion chromatograph is provided between a separation column and a detector of an ion chromatograph. An electrodialysis suppressor includes a first flow path to which an eluent flowing from the separation column is supplied, a second flow path to which a regeneration liquid is supplied, an ion exchange membrane provided between the first flow path and the second flow path and an electrode to which a voltage is applied. A power supply circuit that applies a voltage to the electrode is turned off in a case in which an eluent is not supplied to the first flow path of the electrodialysis suppressor.

The present disclosure provides methods for producing consumable recombinant proteins that are substantially free from herein-disclosed undesired byproducts.

A method exosome purification and characterization is contemplated, comprising the steps of secondary two-stage tangential ultrafiltration to produce an extracted solution, pretreatment of the extracted solution for characterization, characterization of the extracted solution to detect particle size and concentration, and freeze-drying of the extracted solution. An exosome purification integrated device is also contemplated. Through the disclosed methods and devices, exosomes may be better purified and characterized in a manner that results in high practical value to overcome the problems associated with conventional exosome purification processes on the market today, including tedious purification processes, long durations, and high costs.

The present application relates to the technical field of further processing of dairy products, and in particular to a preparation method of milk oligosaccharides, and milk oligosaccharide powder and food prepared thereby. The preparation method comprises the steps of: performing ultrafiltration of whey liquid for at least three times, subjecting the ultrafiltration permeate to nanofiltration concentration for several times, then subjecting the nanofiltration retentate to chromatographic separation and purification, collecting chromatographic collection liquid containing sialyllactose while removing the fraction containing lactose, subjecting the collection to desalination and drying to obtain oligosaccharide powder. The milk oligosaccharides prepared by the present method and the food product containing the same comprise basically bovine milk oligosaccharides, which are light yellow or white in color, light in flavor, uniform in size, and have good thermal stability and solubility. The milk oligosaccharides mainly comprise 3′-sialyllactose and 6′-sialyllactose.

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

A chromatography column includes a first flow distributor, a second flow distributor, and a media chamber having an inlet and an outlet. The second flow distributor is different from the first flow distributor. The first flow distributor is secured to the inlet of the media chamber and the second flow distributor is secured to the outlet of the media chamber.

The present disclosure provides methods for producing consumable recombinant proteins that are substantially free from herein-disclosed undesired byproducts.

The invention relates to a dialysis cell for sample preparation for a chemical analysis method, in particular for ion chromatography. The dialysis cell comprises a donor channel and an acceptor channel extending parallel thereto. The donor channel and the acceptor channel are separated from each other by a selectively permeable dialysis membrane. In particular, an analyte that is dissolved in a donor solution in the donor channel can enter through the dialysis membrane into the acceptor solution in the acceptor channel. The acceptor channel has at least in some sections a volume that is smaller than the volume of the donor channel extending parallel thereto. Acceptor and donor channels are formed from half-cells, between which the dialysis membrane is arranged, wherein the donor channel and the acceptor channel are designed in each case as a recess in a contact surface of one of the half-cells with the dialysis membrane.

A D-allulose syrup including, besides D-allulose, a D-allulose dimer mass content, expressed in terms of dry mass, lower than 1.5%. Also, a method for producing the syrup and to the use thereof for producing food or pharmaceutical products.

The present disclosure is directed to affinity chromatography devices including a fibrillated polymer membrane that contains inorganic particles having a spherical shape and a particle size distribution that has a D90/D10 less than or equal to 3. A blend or a combination of spherical inorganic particles may be utilized. A nominal particle size of the spherical inorganic particles is from about 5 microns to about 20 microns. An affinity ligand may be bonded to the spherical inorganic particles and/or to the fibrillated polymer membrane. Also, the affinity chromatography devices have a hydraulic permeability from about 100 (×10.sup.−12 cm.sup.2) to about 500 (×10.sup.−12 cm.sup.2). Additionally, the affinity chromatography devices have a cycling durability of at least 100 cycles without exceeding an pressure of 0.3 MPa. Manifolds containing multiple affinity chromatography devices in a parallel configuration and multiple manifolds in a parallel configuration are also disclosed.