A crystalline pigment or colorant composition having high color intensity and/or low sugar content, and methods and processes of preparation. The composition may comprise purified fruit and/or vegetable color juices.

The present invention concerns a method for separation of (a) potato proteins and insoluble fibers from (b) first salts and phenolic and/or glycoalkaloid compounds in potato fruit juice or a derivative thereof, said method comprising the steps of: (i) providing a potato fruit juice or a derivative thereof, comprising potato proteins; and insoluble fibers; and one or more first salts; and phenolic and/or glycoalkaloid compounds; (ii) subjecting the potato fruit juice or the derivative thereof to a first cross-flow membrane filtration process resulting in a first retentate and a first permeate; and (iii) adding aqueous diafiltration liquid containing one or more salts to the first retentate and performing a second cross-flow membrane filtration as diafiltration, to create a second permeate containing at least a portion of said phenolic and/or glycoalkaloid compounds and salts and a second retentate comprising potato proteins, salts and insoluble fibers.

The present invention further concerns a potato fruit juice product comprising potato protein and insoluble fibers, such as a potato fruit juice product obtainable by the method according to the invention.

The present invention relates to modification to permeate channels and permeate materials in a cross-flow filtration system to improve performance in counter current filtration having both retentate channels and permeate channels wherein a solution is pumped through one of the channels and drawn through a membrane to one of the other channels to assist in positive pressure driven filtration by using the osmotic pressure, concentration, or preferential solubility difference between the retentate and permeate flow streams thereby increasing or altering the flux through the membrane separating the flow streams.

Methods and devices are disclosed for a separation device. A separation device includes a separation module having a separation membrane separating an interior of the separation module into a retentate compartment and a permeate compartment. The retentate compartment includes at least one retentate channel, a feed port fluidly coupled to the at least one retentate channel and a retentate port. The permeate compartment includes at least one permeate channel disposed within the permeate compartment and a permeate port fluidly coupled to the at least one permeate channel, a retentate collector fluidly connected to the retentate port. The device further includes a feed reservoir, a permeate reservoir, a fluidic gate located between the feed reservoir and the separation module, a vent located between the retentate channel and the permeate channel end adjacent the adjacent the retentate port and a pressure differential source applied across the separation module.

A method for reducing a contaminating DNA content of a preparation containing AAV capsids and contaminating DNA, comprising the steps of a) Performing an extraction of DNA with a solid phase bearing positive charges at its surface said solid phase is contacted with the preparation at a pH of 7.0±1.0, and a salt concentration of 10 mM to 200 mM yielding a first fraction, (b) Diafiltering the first fraction by a first tangential flow filtration to obtain a second fraction, (c) Treating the second fraction with DNase, (d) Diafiltering the DNase treated second fraction obtained by step c) by a second tangential flow, (e) filtration to a buffer with pH of 7.0±1.0, and a salt concentration of 10 mM to 20 mM to yield a third fraction, and optionally (f) Concentrating the third fraction by tangential flow filtration before supplemental chromatography.

The invention concerns a method for the separation of potato proteins from one or more first salts and phenolic and/or glycoalkaloid compounds in potato fruit juice, said method comprising the steps of: (i) providing a potato fruit juice comprising potato proteins, one or more first salts and phenolic and/or glycoalkaloid compounds; (ii) subjecting said potato fruit juice to a first cross-flow membrane filtration process wherein at least a portion of the first salts and at least a portion of the phenolic and/or glycoalkaloid compounds migrate across the membrane into a first permeate and the potato proteins are retained in a first retentate; (iii) adding one or more second salts and water to the first retentate, while continuing the membrane filtration process, to create a diafiltrate containing at least a portion of said phenolic and/or glycoalkaloid compounds and the added second salts and a retentate; and (iv) subjecting the first permeate and/or said diafiltrate from said first cross-flow membrane filtration process to a second cross-flow membrane filtration process.

The present invention concerns a method for separation of (a) potato proteins and optionally insoluble fibers from (b) first salts and phenolic and/or glycoalkaloid compounds in potato fruit juice or a derivative thereof, said method comprising the steps of:

(i) providing a potato fruit juice or a derivative thereof;

(ii) subjecting said potato fruit juice or the derivative thereof to a first cross-flow membrane filtration process resulting in a first permeate and a first retentate;

(iii) adding aqueous diafiltration liquid containing one or more salts to the first retentate and performing a second cross-flow membrane filtration as diafiltration, to create a second permeate being a diafiltrate containing at least a portion of said phenolic and/or glycoalkaloid compounds and salts and a second retentate comprising potato proteins, salts and optionally insoluble fibers;

wherein the pH of the first retentate and the second retentate remains within the range of 4.5 to 8.5 during step (ii) and step (iii), said method further comprising a step of eliminating or reducing enzymatic activity.

Provided herein are materials and methods for the preparation of blood products. In one aspect, provided herein is a composition including platelets or platelet derivatives and an aqueous medium, wherein the aqueous medium has a protein concentration less than 50% of the protein concentration of donor apheresis plasma.

In accordance with the invention, provided herein are methods for purifying recombinant adeno-associated (rAAV) vector particles.

A process for obtaining soluble functional proteins from plant material includes the steps of: mechanically disrupting the cells of the plant material to obtain a mush stream; subjecting the mush stream to a coarse physical separation step, resulting in a permeate and a retentate; subjecting the permeate P.sub.b to mild treatment, resulting in a treated permeate; subjecting the treated permeate to serial centrifugation steps; subjecting centrate to a microfiltration step resulting in a permeate and a retentate; subjecting the permeate to an ultrafiltration step resulting in a permeate and a retentate; subjecting the retentate to hydrophobic column adsorption to provide a column permeate and a retentate; and drying the column permeate to provide a soluble functional protein isolate.