Disclosed are apparatus and methods for blood and other biological fluid purification using a membrane with cell containing vascular channel systems and filtration channel systems. Also disclosed are methods of making the apparatus as well as methods of making membranes.

Methods and devices are disclosed for a separation device. A separation device includes a separation module having a separation membrane separating an interior of the separation module into a retentate compartment and a permeate compartment. The retentate compartment includes at least one retentate channel, a feed port fluidly coupled to the at least one retentate channel and a retentate port. The permeate compartment includes at least one permeate channel disposed within the permeate compartment and a permeate port fluidly coupled to the at least one permeate channel, a retentate collector fluidly connected to the retentate port. The device further includes a feed reservoir, a permeate reservoir, a fluidic gate located between the feed reservoir and the separation module, a vent located between the retentate channel and the permeate channel end adjacent the adjacent the retentate port and a pressure differential source applied across the separation module.

A method for reducing a contaminating DNA content of a preparation containing AAV capsids and contaminating DNA, comprising the steps of a) Performing an extraction of DNA with a solid phase bearing positive charges at its surface said solid phase is contacted with the preparation at a pH of 7.0±1.0, and a salt concentration of 10 mM to 200 mM yielding a first fraction, (b) Diafiltering the first fraction by a first tangential flow filtration to obtain a second fraction, (c) Treating the second fraction with DNase, (d) Diafiltering the DNase treated second fraction obtained by step c) by a second tangential flow, (e) filtration to a buffer with pH of 7.0±1.0, and a salt concentration of 10 mM to 20 mM to yield a third fraction, and optionally (f) Concentrating the third fraction by tangential flow filtration before supplemental chromatography.

A device for obtaining protein-enriched fractions from human or animal milk comprises a delipidating unit for reducing a lipid content in the human or animal milk to obtain delipidated milk and a filtering unit for increasing a protein concentration of the delipidated milk to obtain the protein-enriched fraction, comprising a replaceable filter having a nominal molecular weight limit of 2 kDa or more, in particular of 5 kDa or more.

An inline filter housing with a biodegradable, hydrophilic material that operates in conjunction with a field sampling apparatus to both concentrate field sampled environmental DNA particles from water samples and to automatically preserve the captured DNA via desiccation, thus avoiding filter membrane transfer steps, chemicals or cold storage preservation requirements. The hydrophilic filter housing is capable of rapidly preserving the field sampled environmental DNA captured on the filter membrane at ambient field temperatures.

A disposable cell enrichment kit includes a crossflow filtration device configured to be disposed along a main loop pathway and to receive a process volume containing a biological sample and utilize crossflow filtration, via a micro-porous membrane, to retain a specific cell population in a retentate from the process volume and to remove a permeate including certain biological components from the process volume. The crossflow filtration device includes a laminated filtration unit that includes the micro-porous membrane, a first mating portion, a second mating portion, and a membrane support. The membrane support includes a first plurality of structural features that define a first plurality of openings, wherein the first plurality of structural features are coupled to the micro-porous membrane and provide support to the micro-porous membrane, and the first plurality of openings allow the permeate to flow through them after crossing the micro-porous membrane.

A filter module (10) has a housing (12) which is subdivided by a membrane filter (14) into an inlet chamber (16), which is connected to an inlet connecting piece (22) arranged rigidly on the housing (12), and an outlet chamber (18), which has a filtrate outlet (20). The inlet connecting piece has two connectors, specifically a first connector (26) and a second connector (28), which connect selectively and fluidically to the inlet chamber with a 3-way valve (24) integrated into the inlet connecting piece. The valve has a first entry, which is connected to the first connector, a second entry, which is connected to the second connector, and an exit, which is connected to the inlet chamber. The first connector is configured as an adapter for outwardly sealed coupling of a culture medium bottle (30), which coupling permits a gravity-driven exchange of liquid with the first entry of the valve.

Disclosed is a deflector disc of a disc tube membrane module, including a deflector disc body, radial water distribution ribs, an inner support ring and an outer support ring. Bulges are arranged on the front and back sides of the deflector disc body; first and second ends of the radial water distribution rib are respectively fixedly connected with an inner edge of the deflector disc body and an outer edge of the inner support ring, an annular boss is arranged on the front side of the inner support ring, a seal ring groove is respectively arranged at corresponding positions of the front and back sides of the inner support ring, multiple yielding water collecting grooves are annularly and uniformly distributed on the inner surface of the inner support ring; an inner edge of the outer support ring is fixedly connected with an outer edge of the deflector disc body.

An in vitro release testing (IVRT) device for orally inhaled drug products, for use in an IVRT apparatus, the device having an air-permeable filter loaded with particulate material representing a dose of an orally inhaled drug product. The device has an upper filter support element and a lower filter support element, the loaded filter being circumferentially retained between the upper and lower support elements, a filter cover to cover the upper surface of the loaded filter, and a filter cover retainer provided to assemble and seal the IVRT device.

A device and method for increasing the concentration of an analyte in a fluid sample. The device may include: a housing defining a chamber therein for receiving a fluid sample, a membrane associated with the housing; and a pressure generator operatively connected to the housing to create a pressure gradient across the membrane. When the pressure generator is operated to create the pressure gradient, this causes at least a portion of the fluid sample to move across the membrane. As a result, the fluid sample is separated into a first portion of fluid and a second portion of fluid including said analyte on opposite sides of the membrane. This second portion of fluid by having the analyte present in an amount of fluid that is reduced as compared to the fluid sample prior to the application of pressure - is thus the resulting analyte-concentrated fluid sample.