A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

The present disclosure provides an apparatus for extracting resin from plant material, comprising an agitation tank having an open top and an outlet at a bottom thereof, a strainer basket installed within the agitation tank, the strainer basket comprising walls constructed from a mesh material selected based on a type of plant material to be processed, and an agitator comprising a motor which turns a shaft and two impellers mounted on the shaft. The present disclosure also provides a process for extracting resin from plant material, comprising placing a quantity of plant material within a strainer basket comprising walls constructed from a mesh material, submersing the strainer basket in a tank filled with chilled water at a temperature in the range of 0-10 Celsius, agitating the plant material and chilled water within the strainer basket to separate trichomes from the plant material, and removing a mixture of water and trichomes from an outlet at the bottom of the tank.

A hydrofoil impeller includes a central hub connected to a shaft, and being in the form of a flat plate and being perpendicular to the central axis. The central hub has first bolt holes arranged to form a pattern, the number of groups of first bolt holes corresponding to a number of blades attached to the central hub. At least three blades extend radially outwardly from the central hub, and have a group of second bolt holes arranged in a corresponding pattern to the first bolt holes so that the group of second bolt holes can be aligned with the group of first bolt holes and bolts can be placed through the first and second bolt holes to form bolted joints. The pattern in which the first holes and second holes are arranged in each of the respective groups of holes has a form of a closed curved shape.

In an embodiment, a reactor for carrying out a melt transesterification reaction at a reactor temperature of 160 to 300 C. and a reactor pressure of 5 to 200 mbar, comprises a cylindrical tank comprising a top, a side, and a bottom, wherein the bottom is convex, extending away from the top; a stirring shaft disposed within the cylindrical tank along an axis thereof so that it is rotatable from outside of the cylindrical tank; an impeller extending from the stirring shaft in the cylindrical tank and comprising a plurality of blades; a reactant solution inlet; a reaction solution outlet; and an externally located heat exchanger in fluid communication with the cylindrical tank via a recirculation stream and a heated stream. The reactor can be used for the polymerization of a polycarbonate oligomer.

A fluid heating system includes a tank assembly having an interior surface bounding a chamber, the tank assembly including a sidewall encircling the chamber and extending between a first end and an opposing second end, the first end bounding an opening to the chamber, and a lid movable between a first position wherein the opening to the chamber is exposed and a second position wherein the lid is disposed over the opening. A collapsible bag is removably disposed within the chamber of the tank assembly, the collapsible bag bounding a compartment adapted to hold a fluid. A mechanism is provided for controlling the temperature of fluid within the collapsible bag when the collapsible bag is positioned within chamber of the tank assembly.

A mixing arrangement, and pump for supplying small particles suspended in a liquid to form slurry for delivery includes a container, a mixing and delivery pump having a first shaft, a rotor connected to the first shaft and arranged at an inner bottom of an inner space of the container, a first power device connected to the first shaft, and a stator having stator blades. The stator surrounds the first shaft. The arrangement includes a pump having an inlet, which is located in a stator space between two stator blades, and an outlet, and an outlet pipe having a second inlet opening connected to the outlet and a second outlet opening located outside the inner space.

Disclosed is an impeller for mixing a fluid wherein the impeller is fashioned so as to substantially reduce areas of fluid acceleration and deceleration around a hub region of the impeller during fluid mixing. Additionally, a bioreactor system having microcarriers, kit of parts and cell culturing method are also provided. The impeller has a generally circularly shaped hub having a downwardly directed progressively tapered volume which has plurality of periods formed therein corresponding to the number of increasingly arced blades. Each of the increasingly arced blades flare outwards to a distal end region of the impeller and have an increasing radius to said arc towards a peripheral edge region of said hub. The flare to the increasingly arced blades defines a larger circumference than that of said hub, thereby imparting a generally frusto-conical shape to the impeller. A method mixing a fluid using the impeller disclosed herein is also disclosed.

Stirrer (M) that includes at least two blades and is able to be fixed to a rotary shaft, wherein each blade has a leading edge facing the fluid to be stirred and a trailing edge facing away from the leading edge, and wherein each blade is obtained by bending a flat metal sheet, each blade having two longitudinal bends, the length of each bend being greater than 60% of the maximum radius of the blade.

A hydrofoil impeller includes a central hub connected to a shaft, and being in the form of a flat plate and being perpendicular to the central axis. The central hub has first bolt holes arranged to form a pattern, the number of groups of first bolt holes corresponding to a number of blades attached to the central hub. At least three blades extend radially outwardly from the central hub, and have a group of second bolt holes arranged in a corresponding pattern to the first bolt holes so that the group of second bolt holes can be aligned with the group of first bolt holes and bolts can be placed through the first and second bolt holes to form bolted joints. The pattern in which the first holes and second holes are arranged in each of the respective groups of holes has a form of a closed curved shape.