Acoustofluidic systems including acoustic wave generators for manipulating fluids, droplets, and micro/nano objects within a fluid suspension and related methods are disclosed herein. According to an aspect, an acoustofluidic system includes a substrate including a substrate surface. The system also includes an acoustic wave generator configured to generate acoustic streaming within an acoustic wave region of the substrate surface. Further, the acoustic wave generator is controllable to change the acoustic streaming for movement of a droplet or other micro/nano object on a fluid suspension about the acoustic wave region.

The present invention provides an automatic chemical analysis apparatus and an electrical impedance spectrometry device which make it possible to detect the state of a stirring mechanism prior to performing a stirring operation. The automatic chemical analysis apparatus dispenses a reagent and a sample to be analyzed into a reactor vessel 107, and performs stirring by applying sonic waves to the reactor vessel 107. The automatic chemical analysis apparatus comprises: a piezoelectric element 201 that generates sonic waves; a plurality of split electrodes 211 that are provided to the surface of the piezoelectric element 201; a power amplifier 203 that causes the piezoelectric element 201 to generate sonic waves by applying a voltage to each of the split electrodes 211; and a host computer and impedance measuring circuit that measure the electrical impedance spectrum of each split electrode 211 by applying a voltage to each of the split electrodes 211.

The present invention provides a chemical analyzer with highly reliable agitation performance, said chemical analyzer not only diagnosing the deterioration of a piezoelectric element, but also diagnosing the deformation and displacement of a reaction container and diagnosing the normality of the liquid quantity of a substance to be agitated in the reaction container. This chemical analyzer is characterized by comprising: an agitating mechanism that uses acoustic waves to agitate a sample and a reagent within a reaction container, generates acoustic waves using a piezoelectric element, and has an acoustic wave sensor for detecting the acoustic waves; and a controller that controls the agitating mechanism. Said chemical analyzer is further characterized in that the controller has: an acoustic wave detection unit that processes a detection signal detected by the acoustic wave sensor; a normality information memory in which normal-time information is stored; a signal intensity determination unit that compares the acoustic wave amplitude and acoustic wave frequency transmitted from the acoustic wave detection unit with the acoustic wave amplitude and acoustic wave frequency stored in the normality information memory; and a repeat period determination unit that compares the acoustic wave period characteristic transmitted from the acoustic wave detection unit with the acoustic wave period characteristic stored in the normality information memory.

A sonicator system for sonicating materials in a sample array includes a transducer layer configured to emit acoustic energy; and a multifocus acoustic lens layer configured to focus the acoustic energy from the transducer layer to the sample array to thereby simultaneously sonicate materials in the sample array.

Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

Method and apparatus for controlling acoustic treatment of a sample including a liquid. A processing volume in which the sample is acoustically treated may be controlled, e.g., by positioning a suitable element so as to reduce and/or eliminate a headspace size at a sample/gas interface. An interaction between the acoustic energy and the sample may be controlled, e.g., by using a headspace control element positioned at least partially in the sample that helps to reduce splashing or other sample ejection that would otherwise occur.

A cavitation device and method for using the same is useful for cavitationally treating fluids by generation of hydrodynamic cavitation in the fluid followed by the subsequent collapse of cavitation bubbles. The passage of fluid through slot openings in a cylindrical insert mounted in a housing provides fluid jets in an annular cavity to induce hydrodynamic cavitation of the fluid. Fluid is discharged from the annular cavity into a downstream portion of the housing to collapse cavitation bubbles under static pressure.

Focused ultrasonic acoustic processing is used to prepare formulations particles ranging between approximately 10 nm and approximately 50 microns (e.g., between 1 micron and 20 microns), or between approximately 10 nm approximately 400 nm (e.g., between 10 nm and 100 nm). Formulations (e.g., nanoformulations) may include a suspension (e.g., nanosuspension), an emulsion (e.g., nanoemulsion) or another small particle system. Formulations may be used as delivery systems for therapeutic agents, e.g., a formulation may include a bioactive agent and a carrier compound such as a surfactant that encapsulates the bioactive agent.

Disclosed are mixing apparatus adapted to provide mixing of components in an automated analyzer. The mixing apparatus includes a reservoir configured to contain a coupling liquid, a transducer configured to be driven at a frequency and communicate with the coupling liquid, and a signal generation unit configured to provide a phase modulatable drive signal to the transducer. In some embodiments, improved patient sample and reagent mixing may be provided. Systems and methods are provided, as are other aspects.

Provided is an UFB generating apparatus and an UFB generating method capable of efficiently generating an UFB-containing liquid with high purity. The ultrafine bubble generating apparatus includes a generating unit that generates ultrafine bubbles in a liquid and a post-processing unit that performs predetermined post-processing on the ultrafine bubble-containing liquid generated by the generating unit. The generating unit generates the ultrafine bubbles by causing a heating element, which is provided in the liquid on which the pre-processing is performed, to generate heat to generate film boiling on an interface between the liquid and the heating element.