Function fabrication in a microfluidic device manufactured with a custom 3D printer. The functions may include, for example, transporting or routing fluid, fluid mixing through flow and/or diffusion, blocking fluid (valve), pumping fluid, providing chemical reaction regions, providing analyte capture regions, and providing analyte separation regions. The fluid may be a liquid or a gas.

Disclosed herein is a device (1) for generating a dispersion of a first phase in a second phase, the device comprising a first inlet (2) for supplying a first phase, which opens into a first chamber (4), a second inlet for supplying a second phase, opening into a second chamber and a dispersion outlet (6) for collecting the dispersion. Furthermore, the device comprises a membrane (7), which separates the first chamber (4) and the second chamber (5) and which comprises a first side (8) facing the first chamber (4) and a second side (9) facing the second chamber (5). The membrane (7) comprises multiple channels (10) extending from the first side (8) to the second side (9), providing a fluidic connection between the first chamber (4) and the second chamber (5). Each channel (10) comprises a channel inlet (11) arranged on the first side (8) mid a channel outlet 812) arranged on the second side (9). The first chamber (4) is typically configured such that a flow rate of the first phase through all of the individual channels (10) is essentially equal.

Disclosed herein is a device (1) for generating a dispersion of a first phase in a second phase, the device comprising a first inlet (2) for supplying a first phase, which opens into a first chamber (4), a second inlet for supplying a second phase, opening into a second chamber and a dispersion outlet (6) for collecting the dispersion. Furthermore, the device comprises a membrane (7), which separates the first chamber (4) and the second chamber (5) and which comprises a first side (8) facing the first chamber (4) and a second side (9) facing the second chamber (5). The membrane (7) comprises multiple channels (10) extending from the first side (8) to the second side (9), providing a fluidic connection between the first chamber (4) and the second chamber (5). Each channel (10) comprises a channel inlet (11) arranged on the first side (8) mid a channel outlet 812) arranged on the second side (9). The first chamber (4) is typically configured such that a flow rate of the first phase through all of the individual channels (10) is essentially equal.

A method for mixing liquids using an automated liquid handling system includes: aspirating liquid volumes of a first liquid and a second liquid from alternating ones of a first liquid supply and a second liquid supply into a mixing volume such that the aspirated liquid volumes form a liquid stack including a series of alternating, interfacing layers of the first and second liquids in the mixing volume; permitting the interfacing layers of the first and second liquids to mix with one another by diffusion in the mixing volume to form a mixture liquid; and dispensing the mixture liquid from the mixing volume.

A method for mixing liquids using an automated liquid handling system includes: aspirating liquid volumes of a first liquid and a second liquid from alternating ones of a first liquid supply and a second liquid supply into a mixing volume such that the aspirated liquid volumes form a liquid stack including a series of alternating, interfacing layers of the first and second liquids in the mixing volume; permitting the interfacing layers of the first and second liquids to mix with one another by diffusion in the mixing volume to form a mixture liquid; and dispensing the mixture liquid from the mixing volume.

There is provided an arrangement (100) which allows for mixing a first fluid with a second fluid at a predetermined volume mixing ratio in a capillary driven fluidic system. The arrangement (100) allows filling an initially empty mixing chamber (110) with the first fluid. The arrangement then allows emptying a predetermined fraction of the first fluid from the mixing chamber (110) such as to form an empty space in the mixing chamber (110). The arrangement then allows filling the empty space of the mixing chamber (110) with the second fluid, thereby allowing a predetermined volume of the first fluid to mix with a predetermined volume of the second fluid over time.

There is provided an arrangement (100) which allows for mixing a first fluid with a second fluid at a predetermined volume mixing ratio in a capillary driven fluidic system. The arrangement (100) allows filling an initially empty mixing chamber (110) with the first fluid. The arrangement then allows emptying a predetermined fraction of the first fluid from the mixing chamber (110) such as to form an empty space in the mixing chamber (110). The arrangement then allows filling the empty space of the mixing chamber (110) with the second fluid, thereby allowing a predetermined volume of the first fluid to mix with a predetermined volume of the second fluid over time.

The present disclosure relates to a blood staining patch, a method and device for a blood test using the same, and more particularly, to a patch configured to contain a staining reagent for staining blood and a method and device for economically testing blood using the same. A blood testing method according to an aspect of the present disclosure, which is a blood testing method in which a patch, which includes a mesh structure forming micro-cavities and is configured to contain a staining reagent for staining staining targets present in blood in the micro-cavities, is used to perform a blood test through staining of the staining target, includes placing blood in a reaction region, and providing the staining reagent to the reaction region using the patch configured to contain the staining reagent.

A microfluid device for producing diffusively built gradients comprising a bottom plate and a cover plate, wherein the cover plate has recesses and is connected to the bottom plate in a liquid-tight manner so that the recesses form at least two reservoirs and one observation chamber, which connects the reservoir, a reservoir can be filled particularly through an inlet/outlet through the cover plate, and the cross-sectional surface of the observation chamber is at least 5 times, preferably at least 200 times smaller at the aperture of the observation chamber into one of the reservoirs than the maximum cross-sectional surface of the reservoir in parallel to this cross-sectional surface of the observation chamber.

Nozzle Assemblies and methods of use for producing a liquid jet are disclosed that may be permit adjustable time delays between mixing of fluids and observation of reactions. An example nozzle assembly includes: a housing having an inlet and an outlet and a first channel defined therebetween, where the housing includes a gas focusing aperture defining the housing outlet; an intermediate tube disposed within the first channel of the housing, where the intermediate tube has an inlet and an outlet and defines a second channel therebetween; and a central tube disposed within the second channel of the intermediate tube, where the central tube has an inlet and an outlet and defines a third channel therebetween, where the central tube outlet is longitudinally spaced apart from the intermediate tube outlet such that the intermediate tube outlet is disposed between the central tube outlet and the gas focusing aperture's inlet.