A pneumatic system for a medical fluid machine operating a medical fluid cassette, the pneumatic system including an interface for supplying positive pneumatic pressure and negative pneumatic pressure to the medical fluid cassette; a source of positive pneumatic pressure; a source of negative pneumatic pressure; and a pneumatic pump including a first head and a second head, wherein the first head is dedicated to supplying positive pneumatic pressure to the positive pneumatic pressure source and the second head is dedicated to supplying negative pneumatic pressure to the negative pneumatic pressure source.

The invention relates to a device for synthesising oligonucleotides, comprising: a reagent container receptacle (1) for holding a reagent container support (17) comprising multiple reagent containers (18); an exchangeable microfluid chip (10) comprising a synthesis chamber, fluid connectors and microfluid valves; a control device (5); fluid connecting means (2); wherein the device can be loaded with the microfluid chip (10) and the reagent container support (17) when in a loading position; a chip receptacle (3). To allow cost-effective and prompt synthesis even of small amounts of oligonucleotides, the invention provides for an actuator device (6) to be provided, with which the reagent container receptacle (1), the microfluid chip (10) and the fluid connecting means (2) can be brought from the loading position to an operating position, in which operating position the reagent container receptacle (1), the chip receptacle (3) and the fluid connecting means (2) are positioned relative to each other such that reagents can be conveyed out of the reagent containers (18) towards the synthesis chamber (14) depending on the valve position of the microfluid valves.

The microfluidic devices and systems disclosed herein reduce sample loss and help decrease sample processing bottlenecks for applications such as next generation sequencing (NGS). The microfluidic devices include a plurality of reaction modules. Each reaction module may comprise one or more reaction circuits. Each reaction circuit may comprise a single reaction flow channel with each reaction circuit connected by a bridge flow channel. Alternatively, each reaction circuit may comprise two or more reaction flow channels connected by two or more bridge flow channels. The combination of any two bridge flow channels and a portion of the two or more reaction flow channels between the any two bridge flow channels defining may define the reaction circuit. The reaction module may be arranged as nodes connected by bridge flow channels or each reaction module may be arranged in a parallel fashion on the microfluidic device.

The microfluidic devices and systems disclosed herein reduce sample loss and help decrease sample processing bottlenecks for applications such as next generation sequencing (NGS). The microfluidic devices include a plurality of reaction modules. Each reaction module may comprise one or more reaction circuits. Each reaction circuit may comprise a single reaction flow channel with each reaction circuit connected by a bridge flow channel. Alternatively, each reaction circuit may comprise two or more reaction flow channels connected by two or more bridge flow channels. The combination of any two bridge flow channels and a portion of the two or more reaction flow channels between the any two bridge flow channels defining may define the reaction circuit. The reaction module may be arranged as nodes connected by bridge flow channels or each reaction module may be arranged in a parallel fashion on the microfluidic device.

A pneumatic system for a medical fluid machine operating a medical fluid cassette, the pneumatic system including an interface for supplying positive pneumatic pressure and negative pneumatic pressure to the medical fluid cassette; a source of positive pneumatic pressure; a source of negative pneumatic pressure; and a pneumatic pump including a first head and a second head, wherein the first head is dedicated to supplying positive pneumatic pressure to the positive pneumatic pressure source and the second head is dedicated to supplying negative pneumatic pressure to the negative pneumatic pressure source.

The invention relates to a device for synthesising oligonucleotides, comprising: a reagent container receptacle (1) for holding a reagent container support (17) comprising multiple reagent containers (18); an exchangeable microfluid chip (10) comprising a synthesis chamber, fluid connectors and microfluid valves; a control device (5); fluid connecting means (2); wherein the device can be loaded with the microfluid chip (10) and the reagent container support (17) when in a loading position; a chip receptacle (3). To allow cost-effective and prompt synthesis even of small amounts of oligonucleotides, the invention provides for an actuator device (6) to be provided, with which the reagent container receptacle (1), the microfluid chip (10) and the fluid connecting means (2) can be brought from the loading position to an operating position, in which operating position the reagent container receptacle (1), the chip receptacle (3) and the fluid connecting means (2) are positioned relative to each other such that reagents can be conveyed out of the reagent containers (18) towards the synthesis chamber (14) depending on the valve position of the microfluid valves.

A medical fluid pneumatic manifold system includes a first plate including a plurality of apertures, a second plate attached to the first plate so as to form a plurality of pneumatic flowpaths sealed between the first plate and the second plate, a plurality of tubing connections and a plurality of pneumatic tubes connected to the plurality of tubing connections, the plurality of tubing connections placing the plurality of pneumatic tubes in pneumatic communication with the plurality of pneumatic flowpaths via the plurality of apertures of the first plate, and a pneumatic reservoir in pneumatic communication with the plurality of pneumatic flowpaths, the pneumatic reservoir configured to provide pneumatic pressure to the plurality of pneumatic tubes.

A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

The microfluidic devices and systems disclosed herein reduce sample loss and help decrease sample processing bottlenecks for applications such as next generation sequencing (NGS). The microfluidic devices include a plurality of reaction modules. Each reaction module may comprise one or more reaction circuits. Each reaction circuit may comprise a single reaction flow channel with each reaction circuit connected by a bridge flow channel. Alternatively, each reaction circuit may comprise two or more reaction flow channels connected by two or more bridge flow channels. The combination of any two bridge flow channels and a portion of the two or more reaction flow channels between the any two bridge flow channels defining may define the reaction circuit. The reaction module may be arranged as nodes connected by bridge flow channels or each reaction module may be arranged in a parallel fashion on the microfluidic device.

A medical fluid pneumatic manifold system includes a first plate including a plurality of apertures, a second plate attached to the first plate so as to form a plurality of pneumatic flowpaths sealed between the first plate and the second plate, a plurality of tubing connections and a plurality of pneumatic tubes connected to the plurality of tubing connections, the plurality of tubing connections placing the plurality of pneumatic tubes in pneumatic communication with the plurality of pneumatic flowpaths via the plurality of apertures of the first plate, and a pneumatic reservoir in pneumatic communication with the plurality of pneumatic flowpaths, the pneumatic reservoir configured to provide pneumatic pressure to the plurality of pneumatic tubes.