A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Disclosed is a multi-channel peptide synthesizer, including a gas-bath thermotank, a plurality of reactor tubes, a motor, a rotating rack, a liquid-feeding tube, a feeding device, a vacuum tube and a nitrogen tube. The gas-bath thermotank body provides a desired constant temperature for reaction. The reactor tube provides a place for peptide synthesis and resin washing. The motor and the rotating rack are used to fully mix the reaction and cleaning solutions. Various liquid reagents required are fed to the reactor tube through the liquid-adding tube. Various materials required are prepared in advance in the feeding device and directly fed to the reactor tube. The reaction or washing solution in the reactor tube is pumped to a waste liquid tank through the vacuum tube. Nitrogen is introduced into each reactor tube through the nitrogen tube. This device can be applied in batch-wise peptide synthesis using solid-phase methods.

The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

A fluidic device is described for locally coating an inner surface of a fluidic channel. The fluidic device comprises a first, a second and a third fluidic channel intersecting at a common junction. The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device further comprises a fluid control means configured for creating a fluidic flow path for a coating fluid at the common junction such that, when coating, a coating fluid propagates from the first to the second fluidic channel via the common junction without propagating into the third fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Device for metering powder, in particular in clean-rooms, which includes a vessel containing powder and a sealing head with a septum for the vessel, wherein the sealing head is connectable powder-tight with the vessel and the septum powder-tight with the sealing head and the device further includes a vessel holder, which serves to hold the sealing head of the vessel, and the vessel with its opening points downwards, so that the powder can flow out of the vessel, wherein a gap is provided between the sealing head and a holding bowl of the vessel holder, in which a gas flow between the holding bowl and the sealing head can be created. The invention also relates to a use of the device and a metered addition method.

Device for metering powder, in particular in clean-rooms, which includes a vessel containing powder and a sealing head with a septum for the vessel, wherein the sealing head is connectable powder-tight with the vessel and the septum powder-tight with the sealing head and the device further includes a vessel holder, which serves to hold the sealing head of the vessel, and the vessel with its opening points downwards, so that the powder can flow out of the vessel, wherein a gap is provided between the sealing head and a holding bowl of the vessel holder, in which a gas flow between the holding bowl and the sealing head can be created. The invention also relates to a use of the device and a metered addition method.

A microreactor array platform and method for sealing a reagent in microreactors of an array of microreactors are provided. The microreactor array platform includes an array of microreactors, and a sealing film having a first surface and an opposite second surface, the sealing film configured to movably seal the array of microreactors. The microreactor array platform also includes an injector for delivering a reagent into the array of microreactors via a fluid path between the array and the second surface of the sealing film, and an applicator for directing a sealing liquid against the first surface of the sealing film. The microreactor array platform further includes a system for creating a pressure differential between the reagent in the injector and a space between the array of microreactors and the second surface of the sealing film.