A system for fluorescence activated cell sorting includes at least two excitation lasers and an objective that directs light from the at least two excitation lasers to a common point in an interrogation region of a fluidic channel. The fluidic channel directs a flow of a plurality of fluorescently labeled particles through the interrogation region. At least one modulator temporally multiplexes light from the at least two excitation lasers such that pulses of light from different lasers intersect the common point at different times. The system further includes at least one detector and at least one optical element that directs light emitted from the particles and transmitted through the objective to the at least one detector. The system may further include optics for generating and detecting side and forward scattered light. Methods for operating example systems to collect fluorescent, side scattered and forward scattered light are also described herein.

Provided herein are architectures and compositions of capture templates and macro capture templates, optionally attached to dendrimers, methods of using capture templates and/or macro capture templates, optionally attached to dendrimers to route coding templates, novel combinations including solid supports and capture templates and/or macro capture templates, optionally attached to dendrimers, methods of using novel combinations of solid supports and dendrimers and capture templates and/or macro capture templates to route coding templates, novel compositions which include capture templates and macro capture templates, optionally attached to dendrimers, hybridized to coding templates and novel compositions including solid supports, and capture templates and/or macro capture templates optionally attached to dendrimers hybridized to coding templates.

The present disclosure relates generally to devices and methods useful for generating high-density arrays of target features (e.g., beads) by a permanent magnet and a substrate comprising an array of trapping regions.

Provided herein are methods that comprise providing polymer chains comprising a plurality of reactive groups onto the surface of beads and covalent attachment of functionalized biomolecules, such as primers.

A system for fluorescence activated cell sorting includes at least two excitation lasers and an objective that directs light from the at least two excitation lasers to a common point in an interrogation region of a fluidic channel. The fluidic channel directs a flow of a plurality of fluorescently labeled particles through the interrogation region. At least one modulator temporally multiplexes light from the at least two excitation lasers such that pulses of light from different lasers intersect the common point at different times. The system further includes at least one detector and at least one optical element that directs light emitted from the particles and transmitted through the objective to the at least one detector. The system may further include optics for generating and detecting side and forward scattered light. Methods for operating example systems to collect fluorescent, side scattered and forward scattered light are also described herein.

A system for fluorescence activated cell sorting includes at least two excitation lasers and an objective that directs light from the at least two excitation lasers to a common point in an interrogation region of a fluidic channel. The fluidic channel directs a flow of a plurality of fluorescently labeled particles through the interrogation region. At least one modulator temporally multiplexes light from the at least two excitation lasers such that pulses of light from different lasers intersect the common point at different times. The system further includes at least one detector and at least one optical element that directs light emitted from the particles and transmitted through the objective to the at least one detector. The system may further include optics for generating and detecting side and forward scattered light. Methods for operating example systems to collect fluorescent, side scattered and forward scattered light are also described herein.

Provided herein are methods that comprise providing polymer chains comprising a plurality of reactive groups onto the surface of beads and covalent attachment of functionalized biomolecules, such as primers.