An array including a solid support having a plurality of contours along its exterior surface. A first subset of contours is positioned along the exterior surface of the solid support to form a first pattern of features and a second subset of contours is positioned along the exterior surface to form a second pattern of features. The contours of the first subset are juxtaposed with the second subset along the exterior surface, whereby the first and second patterns form an interleaved pattern. The features of the first pattern occur at a first elevation z.sub.1 and the features of the second pattern occur at a second elevation z.sub.2. The features of the first pattern are configured to attach analytes at a different elevation relative to analytes attached to the features of the second pattern.

An array including a solid support having a plurality of contours along its exterior surface. A first subset of contours is positioned along the exterior surface of the solid support to form a first pattern of features and a second subset of contours is positioned along the exterior surface to form a second pattern of features. The contours of the first subset are juxtaposed with the second subset along the exterior surface, whereby the first and second patterns form an interleaved pattern. The features of the first pattern occur at a first elevation z.sub.1 and the features of the second pattern occur at a second elevation z.sub.2. The features of the first pattern are configured to attach analytes at a different elevation relative to analytes attached to the features of the second pattern.

Disclosed herein are methods and compositions for a new way to separate biologics using coated pegboardsplastic plates affixed with an array of 96-pegs which align with the wells of a traditional 96-well plate used in bench top experiments. The ends of the pegs are conjugated to various compounds which are able to bind tags on engineered proteins (ex. Ni-NTA would bind a His-Tag on an engineered protein). These pegs extend into plate wells to specifically bind the tagged protein from the cell lysate which contains hundreds of other proteins. The pegboard, now laden with the protein of interest (POI), may then be dipped into sequential buffers to wash away potential non-specifically bound proteins to purify the POI.

The present invention relates to the field of chemical synthesis. Specifically, the present invention relates to methods, materials, compositions, and devices for multiplexing chemical synthesis. In particular, the present invention provides novel methods, materials, compositions, and devices to synthesize plurality of chemical compounds, including but not limited to nucleic acids, peptides, saccharides, and phospholipids. Specifically, the present invention provides methods, materials, compositions, and devices to first form plurality of isolated wells on a solid substrate and then to carry out plurality of chemical reactions in the isolated wells, in parallel, and on the same substrate.