A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

An ion exchange device main body 3 includes: a tubular body 31 into which an ion exchange resin bag 5 accommodating the ion exchange resin is inserted through an opening and which has a liquid outlet 312 in which a liquid outlet port 314 for discharging an ion exchange target liquid to outside is formed; a lid 32 that is supported by the tubular body 31 and has a gas injection portion 324 in which a gas injection port 325 for injecting a to an inside 311 of the tubular body 31 is formed; a lead-out pipe 42 that is connected to the liquid outlet 312 and guides the ion exchange target liquid to the outside; and a check valve 44 that is provided in the lead-out pipe 42 and prevents the ion exchange target liquid from flowing backward from the outside to the inside.

An ion exchange device main body 3 includes: a tubular body 31 into which an ion exchange resin bag 5 accommodating the ion exchange resin is inserted through an opening and which has a liquid outlet 312 in which a liquid outlet port 314 for discharging an ion exchange target liquid to outside is formed; a lid 32 that is supported by the tubular body 31 and has a gas injection portion 324 in which a gas injection port 325 for injecting a to an inside 311 of the tubular body 31 is formed; a lead-out pipe 42 that is connected to the liquid outlet 312 and guides the ion exchange target liquid to the outside; and a check valve 44 that is provided in the lead-out pipe 42 and prevents the ion exchange target liquid from flowing backward from the outside to the inside.

Embodiments of the invention provide methods of synthesizing .sup.18F-tetrafluoroborate (.sup.18F-TFB) via direct radiofluorination on boron trifluoride (BF.sub.3) to enhance both labeling yield and specific activity. Uses of .sup.18F-TFB are also contemplated.

The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.

The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.

A water treatment system, such as a water softening system, having a water treatment tank with at least one distributor plate mounted inside to support filter media and/or ion exchange resin. The water treatment system is designed to treat hard water with a packed ion-exchange filter media and has a distributor plate design for facilitating the ion-exchange within a water softener resin bed, as well as facilitating the regeneration of the resin bed. The distributor plate presents cavities to the topside for entrapping filter media, and the cavities have narrow slits located at the base for allowing fluid to pass. A method for assembling the water treatment tank and supporting inserted distributor plate is shown. The distributor plate rest on and is supported by a domed-shaped structure that can be placed in the bottom portion of the water treatment vessel.

A water treatment system, such as a water softening system, having a water treatment tank with at least one distributor plate mounted inside to support filter media and/or ion exchange resin. The water treatment system is designed to treat hard water with a packed ion-exchange filter media and has a distributor plate design for facilitating the ion-exchange within a water softener resin bed, as well as facilitating the regeneration of the resin bed. The distributor plate presents cavities to the topside for entrapping filter media, and the cavities have narrow slits located at the base for allowing fluid to pass. A method for assembling the water treatment tank and supporting inserted distributor plate is shown. The distributor plate rest on and is supported by a domed-shaped structure that can be placed in the bottom portion of the water treatment vessel.

A media tank includes a housing having a first housing portion coupled to a second housing portion at an interface between the first and second housing portions, the first and second housing portions collectively defining an interior volume of the housing, and a divider positioned within the interior volume of the housing at the interface between the first and second housing portions to separate the interior volume into a first chamber at least partially defined by the first housing portion and the divider and a second chamber at least partially defined by the second housing portion and the divider. The first housing portion is coupled to the second housing portion via a friction weld at the interface. An annular gap is provided between the divider and the first housing portion. Flash from the friction weld is received within the annular gap.